Defensin cathelicidin-PP of polypedates puerensis as well as gene and application thereof
A defense peptide, tree frog technology, applied in the field of biomedicine, to inhibit the growth of bacteria and fungi
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[0020] The present invention will be described in detail below in conjunction with the accompanying drawings.
[0021] 1. Preparation and amino acid sequence determination of tree frog defense peptide cathelicidin-PP:
[0022] 1. Collect tree frog skin secretions:
[0023] Live tree frogs were cleaned with water and placed in a cylindrical sample bottle with a diameter of 10 cm and a height of 10 cm. After ether stimulation for 2 minutes, the frog's back foam was rinsed with 10 ml of 0.1M pH 6.0 phosphate buffered saline (PBS). The secreted liquid was centrifuged at 12000 rpm for 15 minutes at 4°C, and the supernatant was stored at -80°C. 2. Separation and purification of tree frog defense peptide cathelicidin-PP:
[0024] Using the supernatant collected above as a raw material, the defense peptide cathelicidin-PP was purified according to the following procedures, and each component of the separation and purification was tested for antibacterial activity. The specific meth...
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