62 results about "Carotene synthesis" patented technology

The invention discloses a recombinant bacterium for producing beta-carotene as well as a construction method and application of the recombinant bacterium. The construction method comprises the following steps: respectively constructing superstrong gene expression modules of protein phytoene synthetase / lycopene cyclase coded by beta-carotene synthetic genes optimized by codons, phytoene dehydrogenase, protein geranyl diphosphate synthase coded by MAV (micro aerial vehicle) pathway genes and 3-hydroxy-3-methylglutaryl coenzyme A reductase; assembling the gene expression modules in vitro, so as to obtain four plasmids of the gene expression modules, integrating the plasmids into a host bacteria genome which does not produce beta-carotene and screening orange single colonies, so as to obtain the recombinant bacterium for producing beta-carotene. The recombinant bacterium disclosed by the invention is adopted for producing beta-carotene by fermental cultivation, and the yield of beta-carotene can reach 26.03mg / g DCW. Therefore, the recombinant bacterium has high performance of producing beta-carotene.

The invention discloses a tobacco carotenoid isomerase gene. The base sequence of the tobacco carotenoid isomerase gene is represented by SEQIDNO:1. In tobacco, a key carotenoid synthesis gene NtCRTISO is cloned, and the gene can be inhibited to effectively improve the carotenoid content of tobacco leaf and improve the photoinhibition resistance of tobacco plants. The invention discloses an important function of the CRTISO gene in tobacco in carotenoid synthesis. The CRTISO gene plays a great role in the regulation of the composition of carotenoid. A method for improving the content of carotenoid, especially beta-carotene, in the tobacco is searched, and a good theoretic guidance is provided for the improvement of the molecules of the plants.

A method used for synthesizing intermediates for use in the synthesis of carotenoid synthetic intermediates, carotenoid analogs, and / or carotenoid derivatives. The analog, derivative, or intermediate may be administered such that a subject's risk of experiencing diseases associated with reactive oxygen species, reactive nitrogen species, radicals and / or non-radicals may be thereby reduced. The analog or analog combination may be administered to a subject for the inhibition and / or amelioration of any disease that involves production of reactive oxygen species, reactive nitrogen species, radicals and / or non-radicals. In some embodiments, the invention may include methods for synthesizing chemical compounds including an analog or derivative of a carotenoid. The carotenoid analog or derivative may be synthetic. In some embodiments, a carotenoid analog or derivative may include at least one substituent. The substituent may enhance the solubility of the carotenoid analog or derivative such that the carotenoid analog or derivative at least partially dissolves in water.

Described herein is a novel three domain gene from Schizochytrium, denoted carotene synthase, that encodes a protein with three different enzymatic activities: phytoene dehydrogenase (PD), phytoene synthase (PS), and lycopene cyclase (LC). Also described is the isolated gene encoding the carotene synthase, homologues thereof, the enzyme encoded by such gene, biologically active portions and homologues thereof, recombinant nucleic acid molecules, microorganisms and plants that have been genetically modified to increase or decrease the action of such gene, and methods of producing carotenoids and derivatives thereof or methods of producing microorganisms and lipid products lacking pigmentation using the knowledge of the carotene synthase described herein.

The invention relates to recombinant bacillus subtilis capable of synthesizing carotenoid 4,4'-diaponeurosporene with 30 carbon atoms (C30), and belongs to the biotechnology genetic engineering field. A bacillus subtilis expression plasmid pMK3-crtmn (which is capable of expressing C30 carotenoid synthases Crtm and Crtn in bacillus subtilis) containing C30 carotenoid synthase genes crtm and crtn is successfully constructed, and is successfully electro-transformed into bacillus subtilis WB800. The WB800 strain after transformation turns from the original color of white to yellow. A pigment component extracted from the recombinant WB800 is identified as 4,4'-diaponeurosporene by high performance liquid chromatography (HPLC). 4,4'-diaponeurosporene can stimulate maturation of dendritic cells, and generated cytokines (IL-6, IL-10, IL-12p70 and TNF[alpha]) have the amount increased, and moreover and have stronger ability to stimulate proliferation of T lymphocytes. With oral administration of the bacillus subtilis capable of synthesizing 4,4'-diaponeurosporene, the dextran sulphate sodium (DSS)-induced mice colitis symptoms can be significantly reduced. The bacillus subtilis capable of producing 4,4'-diaponeurosporene is expected to be developed as probiotics for prevention of colitis.

The invention discloses a genetically engineered bacterium capable of increasing the yield of beta-carotene and application thereof, and the genetically engineered bacterium contains a beta-carotene synthetic pathway related gene, a phosphoketolase pathway related gene and a lipid droplet synthetic pathway related gene. A beta-carotene synthetic pathway is covered; a phosphoketolase pathway is increased, the carbon loss is reduced, and more precursor substance acetyl coenzyme A for synthesizing beta-carotene is generated from a carbon source; the supply of a reducing power substance NADPH and an energy substance ATP is increased; and a lipid droplet synthesis pathway is also increased, and the storage of beta-carotene can be increased by increasing the content and volume of intracellular lipid droplets. The highest yield of the beta-carotene of the genetically engineered bacterium reaches 7.8g / L, and the yield of the beta-carotene is remarkably improved.

The invention discloses a carotenoid synthetic route Tagetes erecta violaxanthin de-epoxidase (TeVDE1) gene. The TeVDE1 is characterized in that the nucleotide sequence of the TeVDE1 gene is shown as SEQ ID NO: 1 in a sequence table, the amino acid sequence of the TeVDE1 gene is shown as SEQ ID NO: 2 in the sequence table. The TeVDE1 gene is obtained from tagetes erecta petal tissue in a clone mode. Real-time quantification analysis and a xanthophyll content determination result show that the expression quantity of the TeVDE1 gene in Tagetes erecta flower buds is high, and the expression level of the TeVDE1 is related to the xanthophyll content. The SEQ ID NO: 1 gene has an effect on xanthophyll synthetic accumulation, and the TeVDE1 obtained from the pigment tagetes erecta in a clone mode can be used for increasing the pigment contents of xanthophylls and other carotenoid.

The invention discloses a cucumber carotenoid phytoene synthetase gene. All cucumber carotenoids are synthesized by isoprenoid compounds or terpenoids. The length of the nucleotide sequence SEQ ID of the cucumber carotenoid phytoene synthetase gene is 1479bp, and the cucumber carotenoid phytoene synthetase gene has a specific nucleotide sequence. The gene-coded polypeptide of claim 1 comprises 422 amino acids, has the molecular weight of 47393.507Da, the isoelectric point of 6.545, the charge of 0.777 when the pH value is 7.0, and has a specific amino acid sequence. The recombinant plasmid pT-PSY of the gene of claim 1 has the length of 4515bp, comprises ampicillin selection markers, and has a specific nucleotide sequence. The invention is used for cloning cucumber carotenoid phytoene synthetase genes.

The invention relates to application of a sll0659 gene to synthesis of synechocystis carotenoids. The nucleotide sequence of the sll0659 gene is shown in SEQ ID No.1. The invention provides application of the sll0659 gene in synechocystis PCC6803, which has an important effect on synthesis of synechocystis PCC6803 beta-carotene. By knocking out the sll0659 gene in synechocystis PCC6803, the lutein content of the synechocystis PCC6803 is increased by 22.3%, zeaxanthin content is increased by 31.3%, the echinenone content is reduced by 34.3%, and the percentage content of beta-carotene is reduced by 1.3%. Meanwhile, HPLC detection results show when synechocystis PCC6803 involved in the invention is compared with wild synechocystis PCC6803, an obvious peak exists in the carotenoid components in the sll0659 gene knockout mutant, and the obvious peak is determined to be alpha-carotene by contrasting the obvious peak with a standard product and detecting.