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Fermentation medium beneficial to synthesis of saccharomyces cerevisiae carotenoid

A technology of fermentation medium and Saccharomyces cerevisiae, which is applied in the field of fermentation medium, can solve the problems such as rare carotenoids, achieve the effect of increasing accumulation, improving success rate, and preventing instability

Active Publication Date: 2021-03-26
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on improving carotenoid production by optimizing medium components

Method used

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  • Fermentation medium beneficial to synthesis of saccharomyces cerevisiae carotenoid
  • Fermentation medium beneficial to synthesis of saccharomyces cerevisiae carotenoid
  • Fermentation medium beneficial to synthesis of saccharomyces cerevisiae carotenoid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Effect of Different Batches of Yeast Extract on Carotenoid Synthesis

[0028] The Saccharomyces cerevisiae fermentation medium of the present embodiment is: YPD medium: yeast extract 10g / L (OXOID company or Angel Yeast Co., Ltd.), peptone 20g / L, glucose 20g / L, solvent is water, its preparation method It is to mix the ingredients evenly according to their content, and sterilize for later use; or YPM medium: yeast extract 10g / L (Angel Yeast Co., Ltd.), peptone 20g / L, glucose 20g / L, salt (potassium dihydrogen phosphate 10g / L, magnesium sulfate heptahydrate 5g / L, potassium sulfate 3.5g / L, sodium phosphate 2.5g / L), TMS solution 1ml / L, solvent is water, and its preparation method is that each composition is mixed according to its content, Sterilized for later use.

[0029] The TMS solution formula is: magnesium chloride hexahydrate 250mg / L, calcium chloride dihydrate 104.5mg / L, copper sulfate pentahydrate 0.4mg / L, sodium iodide 0.08mg / L, manganese chloride tetrahyd...

Embodiment 2

[0033] Example 2 Comparison of Transcriptomes Reveals Potential Mechanisms

[0034] In order to find out what substances in the medium promote the accumulation of carotenoids, we performed transcriptome sequencing on Saccharomyces cerevisiae strains containing Angel Yeast's yeast extract culture conditions and common YPD2 culture conditions, and performed comparative transcriptome analysis. from figure 2 It can be seen that a total of 464 genes were significantly changed (more than 2 times), of which 203 genes were significantly up-regulated and 261 genes were significantly down-regulated. KEGG and GO enrichment analysis found that the glyoxylate pathway, glycolysis pathway, steroid synthesis pathway, fatty acid degradation pathway and pentose phosphate pathway were significantly up-regulated, especially most genes in the ergosterol synthesis pathway were significantly up-regulated. Among them, the HES1 gene was up-regulated by 11 times, the ADY2 gene was down-regulated by 8...

Embodiment 3

[0035] The influence of embodiment 3 copper ions on carotenoid synthesis

[0036] The YPD2 liquid medium of this embodiment is: yeast extract 10g / L, peptone 20g / L, glucose 20g / L, solvent is water, pH is 5.0~7.0, and its preparation method is to mix each component according to its content evenly, adjust pH value, sterilized for later use. Among them, the yeast extract comes from OXOID company, LOT: 2315359-02. YPD2 solid medium is agar added to YPD2 liquid medium.

[0037] Inspired by comparing transcriptomes, the promoting effect of Angel Yeast's yeast extract on carotenoid accumulation may be related to zinc ions and copper ions. So we try to add a variety of nutrients in the medium to investigate its effect on carotenoid synthesis.

[0038] Saccharomyces cerevisiae BL03, Cit1-tHMG1, and ΔAld6 strains were streaked in fresh YPD2 solid medium (the yeast extract was from OXOID company). After 48 hours, a single colony was picked and inoculated in 5ml YPD2 liquid medium, over...

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Abstract

The invention discloses a fermentation medium beneficial to synthesis of saccharomyces cerevisiae carotenoid. The culture medium comprises a saccharomyces cerevisiae basic culture medium and copper ions. The fermentation medium provided by the invention significantly improves the accumulation of recombinant saccharomyces cerevisiae carotenoid (by 10 times). According to the invention, transcriptomics reveals that the addition of zinc ions and copper ions in the culture medium can significantly promote the synthesis of the carotenoid, and the zinc ions, copper ions and the carotenoid have a synergistic enhancement effect.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a fermentation medium beneficial to the synthesis of Saccharomyces cerevisiae carotenoids. Background technique [0002] Carotenoids are a large class of colored substances that occur naturally in plants, algae, fungi, and bacteria. Such substances have strong antioxidant properties and have protective effects on human health, and can be used in food, nutrition, cosmetics, etc. Currently, chemical synthesis and natural extraction are the main ways to prepare carotenoids. However, due to the complexity of the structure, the chemical synthesis process is relatively difficult; and the unstable supply of raw materials, organic solvent pollution, low yield, etc. lead to high cost of natural extraction and cannot be produced on a large scale. Therefore, the use of microorganisms to synthesize specific carotenoids has attracted more and more attention. [0003] With the rapid...

Claims

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Application Information

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IPC IPC(8): C12N1/18C12N1/38C12N15/31C12P23/00C12R1/865
CPCC12N1/18C12N1/38C07K14/395C12P23/00
Inventor 朱红惠苏卜利邓名荣冯广达
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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