34 results about "Photoinhibition" patented technology

The invention relates to the field of biotechnology, and particularly relates to an arachis hypogaca violaxanthin de-epoxidase (AhVDE) gene. A nucleotide sequence is shown by the sequence 1 in a sequence table. An amino acid sequence of the AhVDE gene is shown by the sequence 2 in the sequence table. The invention also discloses an application of the AhVDE gene in a protective photosynthesis mechanism. The AhVDE gene is transfected to tobacco to obtain a transgenic plant; under the stress of excess luminous energy, the transgenic plant has higher NPQ and de-epoxidation state of xanthophyll cycle than a wild plant, thereby indicating that the over-expression of AhVDE changes the relative content of xanthophyll cycle components and leads to the change of the de-epoxidation state under the condition of excess luminous energy, and the heat dissipation capacity of xanthophyll cycle is enhanced. The capacity of the transgenic plant for dissipating the excess luminous energy is enhanced, so that the PSII excitation energy pressure is reduced, the photoinhibition degree of PSII and PSI under the stress of excess luminous energy is relieved, and the photosynthesis mechanism is protected finally.

The invention belongs to the field of microalgae biotechnology, and in particular relates to a method for alleviating the photoinhibition of Haematococcus pluvialis. When the culture of Haematococcus pluvialis cells reaches the plateau stage, when the light is transferred to strong light for light induction, small molecule organic matter glycerol or sodium acetate is added to the culture system, thereby reducing the photoinhibition of algal cells and promoting the production of astaxanthin during the culture process. accumulation. The present invention enhances the ability of Haematococcus pluvialls to resist strong light by adding small molecular organic matter (glycerol or sodium acetate) one or more times during the transition of the cultivation stage, and fully overcomes the strong light resistance of Haematococcus pluvialls. The occurrence of photoinhibition leads to a decrease in cell density, which in turn increases the production efficiency of astaxanthin in Haematococcus pluvialis.

The invention discloses antioxidation-related genes TaSRX-A, TaSRX-B and TaSRX-D of wheat as well as coding proteins and application thereof. The invention also discloses the application of the genes and the coding proteins thereof in regulating the antioxidation function of plants or protecting a photosynthetic system from photoinhibition and photoxidation function. The genes have the advantagesof being capable of clearing away active oxygen in a light stress condition, protecting the photosynthetic system, enhancing the capabilities of resisting photoinhibition and photoxidation of the plants and further increasing the biomass of the plants. The genes and the coding proteins thereof disclosed by the invention have an important theoretical significance and a practical significance for researching light stress resisting mechanisms of common wheat and increasing the output of the common wheat and other important crops and have wide application prospects.

The present invention relates to a new method for avoiding photoinhibition to improve astaxanthin production during microalgae cultivation. The cultivation of microalgae to produce astaxanthin usually adopts a two-stage method. The first stage realizes the mass reproduction of cells and the accumulation of biomass through heterotrophic, autotrophic or polytrophic methods, and the second stage adopts light stress supplemented by nutrient stress and other methods. Accumulation of intracellular astaxanthin is achieved. However, from the first stage to the second stage, the phenomenon of photoinhibition often occurs, which seriously affects the yield of astaxanthin. Therefore, the present invention regulates the cell state by controlling the culture conditions in the transformation process, such as: controlling the composition of the medium, shading, transition to low light, increasing the initial seeding density, gradually diluting, adjusting the outdoor seeding time, etc. Therefore, it fully overcomes the problem of photoinhibition of microalgae under strong light, which can greatly improve the efficiency of microalgae production of astaxanthin, and realize low-cost, high-efficiency and large-scale cultivation of microalgae to produce astaxanthin.

The invention discloses a tobacco carotenoid isomerase gene. The base sequence of the tobacco carotenoid isomerase gene is represented by SEQIDNO:1. In tobacco, a key carotenoid synthesis gene NtCRTISO is cloned, and the gene can be inhibited to effectively improve the carotenoid content of tobacco leaf and improve the photoinhibition resistance of tobacco plants. The invention discloses an important function of the CRTISO gene in tobacco in carotenoid synthesis. The CRTISO gene plays a great role in the regulation of the composition of carotenoid. A method for improving the content of carotenoid, especially beta-carotene, in the tobacco is searched, and a good theoretic guidance is provided for the improvement of the molecules of the plants.

The bean metalloprotease PvFtsH2 gene and its encoded protein and application relate to the field of genetic engineering, in particular to a plant metalloprotease PvFtsH2 gene and its encoded protein and application. The invention provides a bean FtsH metalloprotease PvFtsH2 gene, its encoded protein and application. The nucleotide sequence of the PvFtsH2 gene is shown in SEQ ID NO: 1 in the sequence listing. The amino acid sequence of the encoded protein is shown in SEQ ID NO:2. The present invention confirms that the PvFtsH2 gene plays a role in the light-inhibition response process through plant physiology and molecular biology methods, and the mutation of the gene leads to F under light-inhibition conditions. V / F M The value decreased, the chlorophyll content decreased, and the D1 protein accumulated. The invention has important application value in increasing the yield of kidney beans by improving the utilization rate of light energy.

The invention belongs to the field of gene engineering, and discloses a soybean photosynthesis related gene GmDeg2 and an application thereof. The soybean photosynthesis related gene GmDeg2 has a nucleotide sequence as shown in SEQ ID NO. 1; protein coded by the gene has an amino acid sequence shown in SEQ ID NO.2. By using any vector which can guiding expression of exogenous genes in a plant, the GmDeg2 gene of the invention can be introduced into a plant cell so as to obtain a transgenic plant with improved photoinhibition resistance. The gene of the invention has important significance on soybean photoinhibition resistance, especially on cultivation of soybean varieties with high photosynthetic efficiency.

The invention provides a high-sensitivity coronagraph stray light detection device, which relates to the field of observation and detection of astronomical targets. The optical filter is arranged between the coronagraph to be tested and the single-photon counting detector, and the single-photon counting detector is arranged on the focal plane of the coronagraph to be tested; The simulated sunlight enters the coronagraph to be tested, the band-pass filter performs wavelength selection for the band to be tested, and the single photon counting imaging detector detects the stray light brightness and solar surface brightness of the coronagraph to be tested. The invention can realize the detection of the coronagraph stray light suppression ability by separately measuring the solar surface radiation and the coronagraph stray light radiation, and overcomes the inability to use solar radiation directly to coronagraph stray light suppression in low altitude areas due to the influence of atmospheric scattering. Difficulties in the detection of photoinhibition capacity.

The invention discloses a preparation method of a grating with continuously changing diffraction efficiency: the first beam and the second beam of light with two spectral bands that do not overlap with each other are used, the first beam of light is interfered to form interference light, and the second beam of light Form light with continuous light intensity changes in space; irradiate the interference light and the light with continuous light intensity changes in space on the photosensitive material at the same time, and obtain a grating with continuous change in diffraction efficiency; The material is cured, and the light with continuous light intensity changes in space inhibits the curing of the photosensitive material; or the photosensitive material is a material whose refractive index changes due to light. The preparation method provided by the present invention is directly obtained by exposing the photosensitive material, mainly aiming at the disadvantage of poor uniformity of the outgoing beam of the existing optical system based on the waveguide grating coupler, and can obtain the outgoing beam with uniform light intensity distribution, thereby Effectively improve the imaging capability and information transmission capability of the optical system.

The invention provides a non-toxic biological anti-fouling resistance reducing composite coating, which sequentially comprises a priming paint coating, a luminous powder coating and a protective coating. The protective coating is positioned on a surface layer, the priming paint coating is positioned on a bottom layer, the luminous powder coating is positioned between the protective coating and the priming paint coating, and the surface of the non-toxic biological anti-fouling resistance reducing composite coating is provided with a micron-sized pit structure. The invention further provides a preparation method for the composite coating and an anti-fouling resistance reducing treatment method for the surface of a base material. According to the non-toxic biological anti-fouling resistance reducing composite coating prepared by the preparation method, as luminous powder is capable of illuminating environments, attachment of marine organisms is reduced by means of the photoinhibition effect. On one hand, the luminous powder is non-toxic and radiation-free, and negative effects on the environments are decreased. On the other hand, as the surface of the composite coating is provided with the micron-sized pit structure, the flow field of a near wall can be changed, resistance reduction is realized and coupled with an anti-fouling method, and anti-fouling and resistance reducing functions are realized.