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Application of OsGP3 gene in improving photoinhibition resistance of rice

An anti-photoinhibition, genetic technology, applied in the fields of application, genetic engineering, plant genetic improvement, etc., can solve the problem that the target gene cannot be further translated

Inactive Publication Date: 2016-03-16
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In this way, double-stranded RNA is formed in the cell, and the degradation mechanism is induced to degrade the target RNA, resulting in the inability of the target gene to be further translated, thereby inhibiting the expression of the gene

Method used

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  • Application of OsGP3 gene in improving photoinhibition resistance of rice
  • Application of OsGP3 gene in improving photoinhibition resistance of rice
  • Application of OsGP3 gene in improving photoinhibition resistance of rice

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Experimental program
Comparison scheme
Effect test

Embodiment

[0012] 1. Acquisition of amplification template

[0013] Gene fragments were amplified from cDNA samples of Nipponbare materials for the construction of RNAi vectors. The experimental steps are as follows:

[0014] 1.1 Extraction of total RNA from rice tissue

[0015] ① Take Nipponbare leaf samples at the heading stage, put them into liquid nitrogen quick-freezing quickly, or store them at -80°C. Grind the tissue into powder in liquid nitrogen, add 0.1 g of the sample into a pre-cooled 1.5 mL centrifuge tube, and add 1 mL of TRIzol (Invitrogen), and mix quickly.

[0016] ②Let stand at room temperature for 5-10 minutes, add 200 μL phenol:chloroform:isoamyl alcohol (volume ratio 25:24:1) to each tube of 1 mL TRIzol, shake vigorously for 30 seconds, and let stand at room temperature (25°C) for 5 minutes.

[0017] ③Centrifuge at 12000rpm at 4°C for 10 minutes, the sample is separated into three layers, and the upper aqueous phase is RNA (about 60% of TRIzol). Carefully aspirat...

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Abstract

The invention discloses application of an OsGP3 gene is improving photoinhibition resistance of rice, and belongs to the technical field of plant genetic engineering. According to the invention, a vector constructed through RNAi interference technology is imported into the rice, so that the expression of the OsGP3 gene in the rice is inhibited, and subsequently the functional expression of the OsGP3 gene in a rice plant is researched. A result obtained by comparing with a wild type plant shows that the wild type plant is significantly higher than a transformed plant in NPQ value, showing that the OsGP3 gene plays an important role in photoprotection of the rice; and the OsGP3 gene can be integrated into rice genome through transgenic means, so that the capacity of the rice in consuming excess light energy is enhanced and the effects on protecting the rice and improving crop yield are achieved.

Description

technical field [0001] The invention belongs to the technical field of plant genetic engineering, and in particular relates to the application of OsGP3 gene in improving the ability of rice to resist light inhibition. Background technique [0002] Light is necessary for plant photosynthesis, and the light energy absorbed by chloroplast pigments in the plant photosynthetic mechanism is converted into stable chemical energy through photochemical processes. However, in most cases, the energy received by plants exceeds the energy they can convert. If the excess light energy is not dissipated in time, the photosynthetic function will be reduced, resulting in photoinhibition of photosynthesis, and even photooxidation and photodestruction. When environmental stress factors such as high temperature, low temperature, water deficiency, and nutrient deficiency exist simultaneously, the sensitivity of plants to photoinhibition conditions increases, and photoinhibition occurs under mediu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/53
Inventor 王功伟付湘逵
Owner HUAZHONG AGRI UNIV
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