Tobacco carotenoid isomerase gene and is application
A carotene, isomerase technology, applied in application, genetic engineering, plant genetic improvement and other directions
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[0017] A tobacco carotenoid isomerase gene, the base sequence of which is shown in SEQ ID NO:1.
[0018] The application of the tobacco carotenoid isomerase gene, the tobacco carotenoid isomerase gene increases the tobacco carotenoid content.
[0019] A method for increasing carotenoid content in tobacco comprises the steps of interfering and silencing tobacco carotenoid isomerase gene through transient expression technology to obtain transformed tobacco plants with increased carotenoid content.
[0020] In the method for increasing the content of tobacco carotenoids, the specific nucleic acid fragment of the tobacco carotenoid gene is used as a guide sequence, and the specific nucleic acid fragment is inserted into the plant expression vector in both positive and negative directions to construct The virus-induced gene silencing carrier and RNAi carrier of the tobacco carotenoid gene.
[0021] 1. Homologous cloning and expression pattern analysis of NtCRTISO gene
[0022] Ac...
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