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Peanut Violaxanthin Decycloxygenase Gene, Its Encoded Protein and Its Application

A cyclooxygenase and violaxanthin technology, applied in the biological field, can solve problems such as VDE gene cloning that has not been seen, and achieve the goal of improving the level of deepoxidation, enhancing the ability to dissipate excess excitation energy, and reducing the degree of photoinhibition Effect

Inactive Publication Date: 2016-03-23
BIOTECH RES CENT SHANDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no report on VDE gene cloning and its function research in peanut

Method used

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  • Peanut Violaxanthin Decycloxygenase Gene, Its Encoded Protein and Its Application
  • Peanut Violaxanthin Decycloxygenase Gene, Its Encoded Protein and Its Application
  • Peanut Violaxanthin Decycloxygenase Gene, Its Encoded Protein and Its Application

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Embodiment 1

[0030] 1. Amplify peanut violaxanthin decycloxygenase ( AhVDE )

[0031] RNA was extracted from peanut leaves with RNA kit (purchased from Tiangen Biochemical Technology Co., Ltd.) and reverse transcribed. According to the highly conserved regions of known homologous genes, degenerate primers (5'-CCTGAYGARACKGAATGTC-3', 5'-TACCAGTCATCYTGRTAGTG-3') were designed for polymerase chain reaction (PCR), and the PCR products were analyzed by agarose gel The amplified fragment detected by electrophoresis was consistent with the estimated fragment size (489bp) (see figure 1 A).

[0032] The obtained PCR product was connected with pMD18-T cloning vector, and transformed into Escherichia coli. The positive clones were quickly screened by colony PCR, the plasmids of the positive clones were extracted, and the plasmids were cut using the enzyme cutting sites inside the vector to obtain bands with the same size as the PCR products. Send the identified bacterial liquid to Shanghai Bioen...

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Abstract

The invention relates to the field of biotechnology, in particular to a peanut violaxanthin decycloxygenase gene, the nucleotide sequence of which is shown in sequence 1 in the sequence list. The amino acid sequence of peanut violaxanthin decycloxygenase gene, the amino acid sequence is shown in sequence 2 in the sequence list. Application of peanut violaxanthin decycloxygenase in protecting photosynthetic apparatus. The peanut violaxanthin decycloxygenase gene was transfected into tobacco to obtain transgenic plants. Under excess light energy stress, the transgenic plants had higher NPQ and deepoxidation status of lutein cycle than wild-type plants, indicating that Under the condition of excess light energy, AhVDE The overexpression of lutein changes the relative content of lutein cycle components, which leads to the change of deepoxidation state and improves the heat dissipation capacity of lutein cycle. The enhanced ability of transgenic plants to dissipate excess light energy reduces the pressure of PSII excitation energy, thereby reducing the degree of photoinhibition of PSII and PSI under excess light energy stress, and finally protecting the photosynthetic apparatus.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to peanut violaxanthin decycloxygenase gene ( AhVDE ) and amino acid sequence and its application in photosystem protection. Background technique [0002] Peanut is an important oil crop and economic crop, which plays an important role in my country's agricultural production and even the entire national economy. High temperature and strong light often cause photoinhibition, which affects the accumulation of photosynthetic products in peanuts, which in turn affects the development of pods and reduces yield. With the continuous deterioration of the environment, how to improve the ability of crops to resist light damage has become one of the main goals. The current rapid development of genetic engineering technology provides a new way for plant genetic improvement, genetic transformation using genes that play an important role in the heat dissipation process is an important means to obta...

Claims

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Application Information

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IPC IPC(8): C12N15/53C12N9/02C12N15/11A01H5/00
Inventor 杨莎万书波李新国郭峰孟静静张佳蕾黄超杨连群
Owner BIOTECH RES CENT SHANDONG ACADEMY OF AGRI SCI
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