35 results about "Antibody staining" patented technology

The present disclosure provides an automatic section reading method and system for PD-L1 antibody staining sections. Based on medical digitalimage analysis and processing, the PD-L1 immunohistochemical staining section can be automatically read. In detail, the method disclosed by the invention comprises the following steps: preprocessing the PD-L1 staining digital section image, and obtaining a to-be-analyzed image; inputting the to-be-analyzed image into a pre-established prediction model, and obtaining a target area corresponding to a set cell in the to-be-analyzed image; carrying out imageanalysis on the cell image of each set cell in the target area, identifying the total number of the cells in the target area and the number of the cells which are membrane-positive, and obtaining theproportion of the cells which are membrane-positive in the target area. Finally rapid, accurate and automatic section reading on the set cells is achieved.

A method for evaluating a biological material for unassociated virus-size particles having a particular epitope indicative of an adeno-associated virus viral type or an adenovirus viral type uses a fluorescent antibody stain specific for binding with the epitope and a fluid sample with the virus-size particles and fluorescent antibody stain is subjected to flow cytometry with identification of fluorescent emission detection events indicative of passage through a flow cell of a flow cytometer of unassociated labeled particles of virus size including such a virus-size particle and fluorescent antibody stain.

Described herein are tissue adhesive substrates and methods of making such substrates. Tissue adhesive substrates comprise a glass substrate and a silane covalently bound to the glass substrate. In some variations, the silane may be an amino silane, and may comprise an aryl or an alkyl moiety. In other variations, the silane may be coated with carbon. Silanes may be deposited onto the glass substrate by vapor deposition. Tissue adhesive substrates comprising carbon-coated silanized glass substrates may be capable of retaining a tissue section through multiple cycles of antibody staining and stripping without discernible or substantial damage to the tissue section. In addition, carbon-coated tissue adhesive substrates may also provide a fiduciary marker for closed-loop autofocussing mechanisms.

The invention provides a kit for detecting basophil activation and a using method of the kit. The kit comprises a scrubbing solution, an irritant reference substance, fluorescently-labeled basophil recognition antibodies, red blood cell lysis buffer and an antibody staining solution, wherein the fluorescently-labeled basophil recognition antibodies comprise the following basophil recognition antibodies: Anti human CD 11b, Anti human CD 123, Anti human CD 203c and Anti human CD 63; different basophil recognition antibodies have different fluorescent markers. After the kit is used for detecting the basophil activation situation, the known influence factors of the basophil activation and detection can be avoided, the basophil activation situation can be comprehensively and accurately identified and detected, and a better service is provided for the detection of the basophil activation in the aspect of clinical application and scientific research.

The invention discloses an integrated drug screening and staining method based on a micro-fluidic chip. The micro-fluidic chip is such structured that a liquid path control layer is arranged on the upper layer of the micro-fluidic chip, a gas path control layer is arranged on the lower layer, and a blank glass base plate is arranged on the bottom surface of the micro-fluidic chip. The integrated drug screening and staining method based on the micro-fluidic chip sequentially comprises the following steps: (1) implementing chip pre-processing; (2) inoculating and cultivating cells; (3) implementing drug stimulation; and (4) implementing fluorescent staining. Inlets of all liquid path layers are independently controlled via a valve of a gas path layer; and cultivation of different types of cells, various drug stimulation and different antibody staining can be simultaneously implemented. According to the integrated drug screening and staining method provided by the invention, drug screening and fluorescent staining on the micro-fluidic chip can be achieved via micro-fluid and micro-valve technologies in the micro-fluidic chip, so that a completely new technology platform is provided for researches on cell culture, cell in-situ fluorescent staining and drug staining. The method provided by the invention is simple and convenient to operate, low in cell and reagent dosages, high in integration and broad in application scope.

The invention relates to an application of tumor-derived IgG in diagnosis or prognosis of pancreatic cancer. The invention discloses the application of monoclonal antibodies that specifically recognize a human tumor-derived immune globulin G(CIgG) constant heavy-chain region. The application is characterized by utilizing an immunohistochemical or immunofluorescence technique, determining a pancreatic cancer pathological differentiated degree and patient prognosis according to antibody staining pathological scores, and being used for preparing an immunohistochemistry kit for detecting pancreatic cancer differentiated degree and prognosis evaluation. The application provided by the invention has an important application value for improving the accuracy of pancreatic cancer prognosis evaluation in clinical and scientific research, and provides a basis for individualized diagnosis and treatment scheme of tumor.

The invention discloses a transgenic zebrafish model with macrophages aggregated specifically in liver. The zebrafish refers to the Tg (fabp10a: il34) transgenic zebrafish. The transgenic zebrafish model with macrophages aggregated specifically in liver is realized by the following steps: (1) zebrafish culture; (2) whole-mount in situ hybridization and antibody staining of zebrafish embryos; and (3) laser scanning confocal fluorescence microscopy in vivo imaging. The transgenic zebrafish model with macrophages aggregated specifically in liver proves for the first time that heterotopically expressed and overexpressed interleukin 34 in the liver can induce the macrophages to enrich into the liver, and provides solid and direct evidence by using the in vivo imaging technology. In addition, the Tg (fabp10a: il34; mpeg1: GFP) adult zebrafish has the following phenotypes: (1) the number of the macrophages increases only specifically in the liver region; (2) the number of the macrophages remains unchanged in other tissue regions such as tail hematopoietic tissue (CHT); and (3) the number of granulocytes is unchanged.

The invention provides a staining kit of micro megakaryocytes of a bone marrow smear, and a using method of the staining kit. The staining kit comprises a reagent tube filled with a Polymer Helper reagent, a reagent tube filled with a PBS (Phosphate Buffer Solution), a reagent tube filled with an antigen repairing solution, a reagent tube filled with a hydrogen dioxide solution, a reagent tube filled with diaminobenzidine, a reagent tube filled with hematoxylin, a reagent tube filled with acid alcohol of 1%, a reagent tube filled with ammonium hydroxide of 1%, a reagent tube filled with xylene, a reagent tube filled with alcohol, a reagent tube filled with CD41 monoclonal antibodies, a reagent tube filled with CD61 monoclonal antibodies, a reagent tube filled with universal CD41 and CD61 immunohistochemical antibodies, and a packing box for packing the reagent tubes in a separated and concentrated manner. The staining kit disclosed by the invention overcomes the defect that form cell smears are judged according to subjective experiences, and the interference of false positive of single CD antibody staining to results is avoided, so that the detection accuracy is improved.

The invention provides an automatic slice reading method and an automatic slice reading system for PD-L1 antibody staining slices. The automatic slice reading of PD-L1 immunohistochemical staining slices is realized based on medical digital image analysis and processing. Specifically, the method disclosed by the invention comprises the following steps: preprocessing a PD-L1 dyed digital slice image; obtaining an image to be analyzed, inputting the to-be-analyzed image into a pre-established prediction model; obtaining a target area corresponding to set cells in the to-be-analyzed image; and carrying out image analysis on the cell image of each set cell in the target area, identifying the total number of cells in the target area and the number of cells with positive slice formation, obtaining the proportion of the cells with positive slice formation in the target area, and finally realizing rapid, accurate and automatic slice reading of the set cells.

The invention relates to a second antibody staining system and method, in particular to a second antibody staining system and method for a full-automatic immumohistochemical staining instrument. Reagent components required in conventional immumohistochemical experiments are optimized, the staining power of immumohistochemistry is improved, the probability of the generation of a non-specificity staining background is reduced, and the staining quality is greatly improved; moreover, reagents are loaded in reagent bottles, and do not need to be manually touched, the regent bottles can be opened for use whenever needed while the instrument conducts staining, and great convenience and safety are brought; the second antibody staining system and method can be used for automatic staining of the immumohistochemical staining instrument, and the staining quality totally meets the requirements of hospitals for clinicopathologic diagnosis and the requirements of colleges and universities for scientific research.

The present invention provides a method for cell analysis, comprising: preparing a blood sample comprising nucleated cells having surface, cytoplasmic or nuclear antigens (markers); antibody staining the cell markers; fixing and permeabilising the cells; FISH probe hybridising to chromosomes in the cells; performing imaging flow cytometry on the cells; analysing data obtained from performing imaging flow cytometry; and diagnosing, prognosing or monitoring a medical condition based on the data analysis.

High aldehyde dehydrogenase 1A1 (ALDH1A1) activity has emerged as a reliable marker for the identification of both normal and cancer stem cells. Herein, is presented AlDeSense, a turn-on green fluorescent probe for aldehyde dehydrogenase 1A1 (ALDH1A1) and Ctrl-AlDeSense, a matching non-responsive reagent. AlDeSense exhibits a 20-fold fluorescent enhancement when treated with ALDH1A1. Through the application of surface marker antibody staining, tumorsphere assays, and assessment of tumorigenicity, the disclosed results show that cells exhibiting high AlDeSense signal intensity have properties of cancer stem cells. Herein, is also reported the development of a red congener, red-AlDeSense. Importantly, red-AlDeSense represents one of only a few examples of a turn-on sensor in the red region using the d-PeT quenching mechanism.

A method of detecting the presence of bacteria in milk is provided, wherein milk is contacted with a preparation which may comprise an effective amount of at least one type of antibodies, said antibodies being capable of specifically binding to bacteria to be detected; staining said antibodies, before or after said contacting, with a staining preparation, said staining preparation being selected so that the antibodies stained therewith exhibit a color change or a change in color concentration when in contact with said bacteria; and determining the concentration of stained antibodies bound to bacteria in said milk from the presence and / or the relative intensity of the color change caused by contacting said milk with said stained antibodies.

The invention relates to a secondary antibody staining method, in particular to a secondary antibody staining method for a fully-automatic immunohistochemical staining apparatus. The secondary antibody staining method comprises the following steps of S1, adding a blocking fluid into a tissue slice, and deactivating endogenous peroxidase in cells of the tissue slice; S2, adding a corresponding primary antibody into the tissue slice, and reacting and combining with antigen protein in the cells of the tissue slice; S3, adding a reaction reinforcing solution into the tissue slice; S4, adding a polymer secondary antibody into the tissue slice, and specifically combining with the primary antibody; S5, adding a mixed solution of DAB (diaminobenzidine) concentration solution and DAB buffer solution into the tissue slice, and precipitating and staining DAB; S6, adding a hematoxylin redyeing solution into the tissue slice, combining with chromatin, and displaying blue color. The secondary antibody staining method has the advantages that the required reagent components in the conventional immunohistochemistry experiment are optimized, the staining intensity of immunohistochemistry is improved, the possibility of production of non-specific staining background is reduced, and the staining quality is greatly improved.

The present invention provides a method for cell analysis, comprising: preparing a blood sample comprising nucleated cells having surface, cytoplasmic or nuclear antigens (markers); antibody stainingthe cell markers; fixing and permeabilising the cells; FISH probe hybridising to chromosomes in the cells; performing imaging flow cytometry on the cells; analysing data obtained from performing imaging flow cytometry; and diagnosing, prognosing or monitoring a medical condition based on the data analysis.

An immunofluorescent staining method for synapse sites in a nervous system is provided. The method includes steps of (1) selecting, (2) quick-freezing, (3) immobilizing and (4) antibody staining. The method is advantaged by a high freezing efficiency, a low cost, capability of effectively maintaining integrity of cell structures, good immobilizing effects, convenient staining operation and a high staining rate.

The invention relates to using CUT&RUN to validate CRISPR-Cas targeting. To improve the efficiency of CRISPR-based gene editing and delivery for in vivo applications, a method which may comprise: (a) expressing a catalytically inactive Cas protein (dCas) in target cells, (b) optional hypotonic lysis of the cells of step (a) to release nuclei, (c) immobilizing cells of step (a) or nuclei of step (b) with magnetic beads, (d) incubating the product of step (c) with an anti-CRISPR-dCas antibody, (e) incubating the product of step (d) with ProteinA-MNase (pAG-MNase), (f) adding of a Ca2+ ions-containing buffer to start MNase digestion and release of pAG-MNase-antibody-chromatin complexes, (g) adding a chelator-containing buffer to stop the reaction of step (f), (h) pelletizing the obtained oligonucleosome and obtaining pAG-MNasebound digested chromatin fragments from the supernatant, (i) extracting of DNA and RNA from the chromatin fragments of step (h), and (j) sequencing of DNA and RNA.

The invention provides a kit for detecting human regulatory T cell subtypes and a detection method thereof, belonging to the technical field of cell subtype detection. The kit provided by the invention includes the following components: blood dilution solution, mononuclear cell separation solution, cell culture solution, lymphocyte activation solution, dead cell removal dye, FcR blocking agent, fluorescently labeled anti-human cell surface marker molecule Antibodies, fluorescently labeled antibodies against human intracellular molecules, PBS buffer, lipopolysaccharide, washing buffer, cell staining buffer, cell fixative, and permembrane wash. During the detection, the mononuclear cells in the whole blood are first isolated, and then the human peripheral blood mononuclear cells are stimulated, and finally the regulatory T cell subtypes can be detected by fluorescent antibody staining. By using the kit and detection method of the present invention, 2-6 subtypes of regulatory T cells can be easily and quickly distinguished.

The invention provides a kit for detecting basophil activation and a using method of the kit. The kit comprises a scrubbing solution, an irritant reference substance, fluorescently-labeled basophil recognition antibodies, red blood cell lysis buffer and an antibody staining solution, wherein the fluorescently-labeled basophil recognition antibodies comprise the following basophil recognition antibodies: Anti human CD 11b, Anti human CD 123, Anti human CD 203c and Anti human CD 63; different basophil recognition antibodies have different fluorescent markers. After the kit is used for detecting the basophil activation situation, the known influence factors of the basophil activation and detection can be avoided, the basophil activation situation can be comprehensively and accurately identified and detected, and a better service is provided for the detection of the basophil activation in the aspect of clinical application and scientific research.

The present invention relates to a composition for clearing biotissue and a clarity method for biotissue using the same. The composition for clearing biotissue and the clarity method for biotissue using the same of the present invention do not need a high price electrophoresis apparatus and expensive solutions, and can not only be applied to various biotissues including brain, liver, lung, kidney, intestine, heart, muscle, and blood vessel, without damaging any of them but also prevent bubble formation, color change, and dark sediment; improve the clarity of biotissues; and help antibody-staining of the cleared tissues. Therefore, it is useful to identify the reason of various diseases through structural imaging of biotissue and to establish a treatment method.

The invention discloses a sample pretreatment method based on flow combination ICP-MS (Inductively Coupled Plasma Mass Spectrometry) single cell protein detection, and belongs to the technical field of sample pretreatment of flow cytometry. The sample pretreatment method comprises the following steps: (1) collecting and transporting a whole blood sample; (2) performing PBMC (Peripheral Blood Mononuclear Cell) cell separation; (3) performing cell active dyeing; (4) performing cell stimulation; (5) performing cell immobilization; (6) performing surface antibody dyeing; (7) performing intracellular phosphorylated protein dyeing; (8) performing single cell labeling, and the like. With the combination of a metal element labeled antibody with cell surface antigen, labeled cells are mixed with beads as internal reference for standardization, dead cells and living cells are distinguished in the pretreatment process, then the situation that the testing result analysis and description are affected by too many dead cells is avoided, single cells are distinguished from cell dimmers, cell trimers or even cell multimers, and flow combination ICP-MS single cell protein detection requirements can be very well met.

The invention discloses an immunofluorescence kit for detecting circulating prostate epithelial cells in blood and a use method of the immunofluorescence kit, and belongs to the technical field of molecular biology. The immunofluorescence kit for detecting circulating prostate epithelial cells in blood comprises a stationary liquid, a confining liquid, a cell membrane permeation liquid, an antibody staining liquid, a fluorescent dye, a DAPI staining liquid and a mounting liquid. Wherein the antibody staining solution is a mixed solution of a mouse anti-CK15 antibody and a rat anti-CD45 antibody. The invention also discloses a use method of the immunofluorescence kit for detecting circulating prostate epithelial cells in blood. According to the immunofluorescence kit, the combination of the mouse anti-CK15 antibody and the rat anti-CD45 antibody is adopted, prostate epithelial cells dissociating into blood can be screened out with high specificity, and the detection rate of benign prostatic hyperplasia and prostatic cancer is high.

The invention provides a liquid phase staining identification method used for cells and needing extremely small cell quantity. The method comprises the following steps: (1) adding a cell immobilizingsolution into cells in a non-dry state for cell immobilization; (2) after cleaning the cells, adding a cell staining solution for cell staining, wherein the cell staining solution comprises a fluorescent antibody staining solution; (3) dyeing the cells by utilizing bright-field light dyeing liquid; and (4) after cleaning the cells, suspending the cells, and observing the cells under fluorescence and bright-field light via a microscope so as to simultaneously obtaining various fluorescence and bright-field light images. According to the invention, on the premise that cells are not dried or sealed, staining and imaging via dual light sources of fluorescence antibody and bright field optical cell morphology can be carried out, and high-quality fluorescence markers and bright-field optical morphological images of the cells can be obtained; and the method disclosed by the invention is simple and feasible in process, low in cost and beneficial for cell classification and subsequent molecular-level analysis, and more comprehensive cell marker information can be obtained.

The invention discloses an atohla expression diagram method in a zebra fish lateral line hair cell regeneration process. The method utilizes an atohla antisense nucleotide probe, by using a whole-mount in situ hybridization method, the atohla expression diagram is observed in the process that the fish lateral line hair cells die and regenerate under the induction of cis-platinum, the spatial relationship between expression cell groups of the atohla and nerve-hillock center mature hair cells is observed by using the method of co-dyeing of hybridization in situ and antibody staining, a Notch signal inhibitor is added through an outer source to further observe the change of regenerated hair cell quantity under the circumstance that atohla expression is increased, and in vivo experimental evidence is provided to determine that cell groups marked by the atohla are precursor cells in the zebra fish lateral line hair cell regeneration process.

The invention belongs to the technical field of cell staining and kits, and particularly relates to an HDN staining solution and a kit thereof for specific color development of horseradish peroxidaseby using the HDN staining solution. The kit comprises the HDN staining solution, wherein the HDN staining solution comprises hydrogen peroxide, a buffer solution, ammonium nickel sulfate, 3, 3'-diaminobenzidine and ammonium chloride. An immunohistochemistry method of the kit comprises the following steps of: (1) slicing and fixing a detected living tissue; 2) detecting a to-be-discovered antigen or antibody by using a primary antibody; 3) carrying out staining, specifically, immersing the obtained tissue slice into the HDN staining solution, wherein a positive detection object of the tissue slice is dark purplish blue; 4) cleaning the tissue slice; 5) and sealing the slice, and observing the tissue slice. By adopting the HDN staining solution for staining, the obtained sensitivity of the kit is more than 10 times higher than the sensitivity obtained by using the conventional method, the substance which cannot be stained by using the conventional method can be displayed, the false negative result can be reduced, the missed diagnosis can be avoided, and the false positive result can be avoided since the non-positive substance is not stained.

The invention relates to the technical field of veterinary drug preparation, in particular to an immunohistochemical antigen repair buffer solution and a use method thereof. Each liter of the antigen repair buffer solution contains 1.35 to 1.62 g of Tris-Base, 0.45 to 0.55 g of trans-2-methyl-2-butenedioic acid, 0.8 to 1.2 g of mannosylerythritol, 0.16 to 0.20 g of trypsin, 0.20 to 0.25 g of an aloe extract, 0.12 to 0.16 g of a water-retaining agent, 0.08 to 0.12 g of sodium diacetate, 0.4 to 0.6 g of a surfactant and the balance of distilled water. According to the antigen repair buffer solution provided by the invention, the antigenicity lost in the processes of flaking and the like can be recovered to the greatest extent, so that a good foundation is provided for subsequent antibody staining; the phenomenon that the tissue slice is dried due to excessive volatilization can be effectively prevented, so that the damaged antigen can be smoothly repaired; and besides, cross-linking between proteins caused by aldehyde fixing reagents can be effectively removed, and antigen epitopes in samples such as paraffin sections and the like are fully exposed, so that the immunostaining effect is greatly improved.

The invention, which relates to the technical field of poultry hazard detection, discloses a rapid detection method for swine fever, thereby solving the problem that a small amount of fat and proteinimpurities exist in a sample in the detection process, so that more errors are easily generated in detection. The rapid detection method comprises the following steps: step 1, carrying out crushing and grinding; step 2, carrying out centrifugal filtration; step 3, carrying out precipitation and impurity removal; step 4, carrying out inoculation culture; step 5, carrying out fluorescent antibody staining; and step 6, carrying out observing and comparing. In the actual application process, the rapid detection method for the swine fever can be used for effectively eliminating the interference offat and protein impurities in the detection process and has the advantages of being high in detection speed, high in accuracy and small in error.

The invention discloses a spatial transcriptome data processing method and device and a computer readable storage medium. The method comprises the following steps: acquiring a Hamp of a target sample slice through a space transcriptome technology; e, dyeing an image and a space transcriptome sequencing analysis result; performing fluorescence-labeled antibody staining of the target protein on the target sample section through a fluorescence staining method to obtain a fluorescence staining image of the target protein; the method comprises the following steps: performing Hamp; the E dyeing image and the fluorescent dyeing image are superposed to obtain a superposed image; extracting site information corresponding to a fluorescence positive signal in the superposed image; and analyzing and processing the transcriptome data corresponding to the site information according to the spatial transcriptome sequencing analysis result to obtain a spatial transcriptome data analysis result of the target sample slice at the site information. According to the method and the device, the technical problem that the reliability is relatively low as only a space transcriptome technology combined with single morphological information is adopted to analyze the sample in the related technology is solved.

The invention discloses a Th detecting method for a non-heparin anticoagulant blood sample. In the method, a non-heparin anticoagulant sample is processed by using a lymphocyte separation solution toobtain peripheral blood mononuclear cells (PBMC), the effect of the anticoagulant on cell activation and cytokine secretion is removed, and the serum is added at the same time in the stimulation / blocking stage to provide cells with sufficient nutrients. After antibody staining and fixed membrane rupture, the method can be successfully used for the detection of Th cytokines, thereby expanding the range of Th detection sample type, enables researchers or testers to make full use of samples for Th detection, avoids waste and repeated collection of samples caused by the collection of non-heparin anticoagulant samples, and greatly improves efficiency.