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Method for detection of bacteria in milk

A technology for bacteria and to-be-detected, applied in biochemical equipment and methods, measuring devices, determination/inspection of microorganisms, etc., and can solve problems such as low price

Inactive Publication Date: 2014-10-29
谢策·谢泽马
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Excessively high bacterial concentrations in shipped milk are an indication of mastitis, which results in milk producers receiving lower prices per unit of milk shipped

Method used

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Examples

Experimental program
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Effect test

Embodiment Construction

[0019] Mastitis results in an increased bacterial load per unit weight or unit volume of milk. Bacteria can be counted using fluorescence techniques using the principle of flow cytometry.

[0020] Fluorescence in this context refers to combining (and thereby reacting) bacteria with appropriately stained antibodies and passing the combination through a flow cell where under the influence of fluorescence individual cells combined with antibodies emit pulses of light. The number of light pulses is detected, which correlates with the bacterial cell number.

[0021] There are multiple groups of bacteria that cause mastitis. The present invention is based on the fact that specific antibodies bind only to specific types of bacteria. From this data, different bacteria can be identified by using different antibodies.

[0022] A single type of antibody can be used to detect and measure the concentration of essentially a single type of bacteria. However, since generally more than one...

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PUM

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Abstract

A method of detecting the presence of bacteria in milk is provided, wherein milk is contacted with a preparation which may comprise an effective amount of at least one type of antibodies, said antibodies being capable of specifically binding to bacteria to be detected; staining said antibodies, before or after said contacting, with a staining preparation, said staining preparation being selected so that the antibodies stained therewith exhibit a color change or a change in color concentration when in contact with said bacteria; and determining the concentration of stained antibodies bound to bacteria in said milk from the presence and / or the relative intensity of the color change caused by contacting said milk with said stained antibodies.

Description

Background technique [0001] Some bacteria cause diseases (especially mastitis) in dairy cows which can adversely affect the quality of the milk. For example, mastitis is an infectious disease in the udder or teat of an individual dairy cow, and mastitis bacteria are often present in the milk produced by such cows. Many different types of bacteria may be involved in causing mastitis, ie there is not just one type or strain of mastitis bacteria but many. The types of bacteria involved in mastitis generally vary from case to case. [0002] Because dairy products are generally (especially in industrial-scale dairy operations) produced by blending milk from multiple cows, when some cows become infected, the dairy product will be Contains a certain amount of undesired microorganisms (for example, mastitis-causing bacteria). However, there is no a priori predictability of the type and concentration of bacterial cells contained in infected milk that is ultimately mixed into the fin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569
CPCG01N33/56911C12Q2304/00C12Q1/06
Inventor 谢策·谢泽马
Owner 谢策·谢泽马
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