Staining kit of micro megakaryocytes of bone marrow smear, and using method of staining kit

Abstract

The invention provides a staining kit of micro megakaryocytes of a bone marrow smear, and a using method of the staining kit. The staining kit comprises a reagent tube filled with a Polymer Helper reagent, a reagent tube filled with a PBS (Phosphate Buffer Solution), a reagent tube filled with an antigen repairing solution, a reagent tube filled with a hydrogen dioxide solution, a reagent tube filled with diaminobenzidine, a reagent tube filled with hematoxylin, a reagent tube filled with acid alcohol of 1%, a reagent tube filled with ammonium hydroxide of 1%, a reagent tube filled with xylene, a reagent tube filled with alcohol, a reagent tube filled with CD41 monoclonal antibodies, a reagent tube filled with CD61 monoclonal antibodies, a reagent tube filled with universal CD41 and CD61 immunohistochemical antibodies, and a packing box for packing the reagent tubes in a separated and concentrated manner. The staining kit disclosed by the invention overcomes the defect that form cell smears are judged according to subjective experiences, and the interference of false positive of single CD antibody staining to results is avoided, so that the detection accuracy is improved.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to bone marrow smear staining technology, in particular to a bone marrow smear micromegakaryocyte staining kit and its application method which are jointly marked with CD41 and CD61 antibodies. Background technique [0002] Micromegakaryocytes are usually pathological megakaryocytes. The diameter of micromegakaryocytes is less than 15 μm. The cells are comparable in size to lymphocytes, with less cytoplasmic content, and are often accompanied by platelet production. Microscopic aggregated cells are seen in many blood diseases and are one of the characteristics of bone marrow abnormal hematopoiesis. Clinically, Wright-Giemsa staining method is commonly used to stain and identify micromegakaryocytes, but this staining method is judged by subjective experience through morphological cell smears, and it is easy to confuse micromegakaryocytes with primitive megakaryocytes and lymphocytes, so cl...

AI Technical Summary

Problems solved by technology

Clinically, Wright-Giemsa staining method is commonly used to stain and identify micromegakaryocytes, but this staining method is judged by subjective experience through morphological cell smears, and it is easy to confuse micromegakaryocytes with primitive megakaryocytes and lymphocytes, so clinical The detection accuracy is low

At present, there is also a CD41 monoclonal antibody bone marrow cytology detection technology, but this technology has the disadvantage of false positives, which makes the accuracy of the detection results low.