Staining kit of micro megakaryocytes of bone marrow smear, and using method of staining kit

A bone marrow smear and megakaryocyte technology, applied in the biological field, can solve the problems of confusion of primitive megakaryocytes and lymphocytes, low accuracy of test results, and low accuracy, so as to improve clinical diagnosis and differential diagnosis, and improve positive detection efficiency, and the effect of improving accuracy

Inactive Publication Date: 2015-08-26
北京海思特医学检验实验室有限公司
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Problems solved by technology

Clinically, Wright-Giemsa staining method is commonly used to stain and identify micromegakaryocytes, but this staining method is judged by subjective experience through morphological cell smears, and it is easy to confuse micromegakaryocytes with primitive megakaryoc...

Method used

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Embodiment

[0026] Bone marrow smear micromegakaryocyte staining kit and its application method jointly labeled with CD41 and CD61 antibodies

[0027] 1. The kit contains:

[0028] Reagent tubes containing Polymer Helper reagent, reagent tubes containing PBS phosphate buffer solution, reagent tubes containing antigen retrieval solution, reagent tubes containing hydrogen peroxide solution, reagent tubes containing diaminobenzidine staining solution , reagent tubes containing hematoxylin staining solution, reagent tubes containing 1% acid alcohol, reagent tubes containing 1% ammonia water, reagent tubes containing xylene, reagent tubes containing alcohol, CD41 monoclonal antibody Reagent tubes, reagent tubes containing CD61 monoclonal antibody, reagent tubes containing general-purpose CD41 and CD61 immunohistochemical antibodies; packaging boxes for separating and collectively packaging these reagent tubes.

[0029] The above-mentioned Polymer Helper reagent, antigen retrieval solution, di...

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Abstract

The invention provides a staining kit of micro megakaryocytes of a bone marrow smear, and a using method of the staining kit. The staining kit comprises a reagent tube filled with a Polymer Helper reagent, a reagent tube filled with a PBS (Phosphate Buffer Solution), a reagent tube filled with an antigen repairing solution, a reagent tube filled with a hydrogen dioxide solution, a reagent tube filled with diaminobenzidine, a reagent tube filled with hematoxylin, a reagent tube filled with acid alcohol of 1%, a reagent tube filled with ammonium hydroxide of 1%, a reagent tube filled with xylene, a reagent tube filled with alcohol, a reagent tube filled with CD41 monoclonal antibodies, a reagent tube filled with CD61 monoclonal antibodies, a reagent tube filled with universal CD41 and CD61 immunohistochemical antibodies, and a packing box for packing the reagent tubes in a separated and concentrated manner. The staining kit disclosed by the invention overcomes the defect that form cell smears are judged according to subjective experiences, and the interference of false positive of single CD antibody staining to results is avoided, so that the detection accuracy is improved.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to bone marrow smear staining technology, in particular to a bone marrow smear micromegakaryocyte staining kit and its application method which are jointly marked with CD41 and CD61 antibodies. Background technique [0002] Micromegakaryocytes are usually pathological megakaryocytes. The diameter of micromegakaryocytes is less than 15 μm. The cells are comparable in size to lymphocytes, with less cytoplasmic content, and are often accompanied by platelet production. Microscopic aggregated cells are seen in many blood diseases and are one of the characteristics of bone marrow abnormal hematopoiesis. Clinically, Wright-Giemsa staining method is commonly used to stain and identify micromegakaryocytes, but this staining method is judged by subjective experience through morphological cell smears, and it is easy to confuse micromegakaryocytes with primitive megakaryocytes and lymphocytes, so cl...

Claims

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Application Information

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IPC IPC(8): G01N33/532
CPCG01N33/532
Inventor 刘建勇刘佳张鑫王绪华梁超陈忠方国伟黄士昂
Owner 北京海思特医学检验实验室有限公司
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