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Spatial transcriptome data processing method and device and computer readable storage medium

A technology of spatial transcription and processing method, applied in the field of data processing, can solve problems such as low reliability, and achieve the effect of improving reliability

Pending Publication Date: 2022-04-01
天津诺禾致源生物信息科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Embodiments of the present invention provide a method and device for processing spatial transcriptome data, and a computer-readable storage medium, so as to at least solve the problem of relatively low reliability in the analysis of samples by spatial transcriptome technology combined with a single morphological information in the related art. low technical issues

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  • Spatial transcriptome data processing method and device and computer readable storage medium
  • Spatial transcriptome data processing method and device and computer readable storage medium
  • Spatial transcriptome data processing method and device and computer readable storage medium

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Embodiment 1

[0030] According to an embodiment of the present invention, a method embodiment of a method for processing spatial transcriptome data is provided. It should be noted that the steps shown in the flowchart of the accompanying drawings may be implemented in a computer system such as a set of computer-executable instructions. and, although a logical order is shown in the flowcharts, in some cases the steps shown or described may be performed in an order different from that herein.

[0031] figure 1 is a flowchart of a method for processing spatial transcriptome data according to an embodiment of the present invention, such as figure 1 As shown, the processing method of the spatial transcriptome data includes the following steps:

[0032] In step S102, the H&E staining image of the target sample slice and the analysis result of spatial transcriptome sequencing are obtained by using the spatial transcriptome technology.

[0033] In step S104, the target sample section is stained w...

Embodiment 2

[0055] According to another aspect of the embodiments of the present invention, an apparatus for processing spatial transcriptome data is also provided, Figure 4 is a schematic diagram of a processing device for spatial transcriptome data according to an embodiment of the present invention, such as Figure 4 As shown, the apparatus for processing spatial transcriptome data may include: a first acquiring unit 41 , a staining unit 43 , a superimposing unit 45 , an extracting unit 47 and a second acquiring unit 49 . The processing apparatus for the spatial transcriptome data will be described below.

[0056] The first obtaining unit 41 is configured to obtain the H&E staining image of the target sample slice and the analysis result of spatial transcriptome sequencing by using the spatial transcriptome technology.

[0057] The staining unit 43 is configured to stain the target sample section with a fluorescently labeled antibody of the target protein by a fluorescent staining me...

Embodiment 3

[0070] According to another aspect of the embodiments of the present invention, a computer-readable storage medium is also provided, where the computer-readable storage medium includes a stored computer program, wherein when the computer program is executed by the processor, a device where the computer-readable storage medium is located is controlled The processing method for spatial transcriptome data of any one of the above is performed.

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Abstract

The invention discloses a spatial transcriptome data processing method and device and a computer readable storage medium. The method comprises the following steps: acquiring a Hamp of a target sample slice through a space transcriptome technology; e, dyeing an image and a space transcriptome sequencing analysis result; performing fluorescence-labeled antibody staining of the target protein on the target sample section through a fluorescence staining method to obtain a fluorescence staining image of the target protein; the method comprises the following steps: performing Hamp; the E dyeing image and the fluorescent dyeing image are superposed to obtain a superposed image; extracting site information corresponding to a fluorescence positive signal in the superposed image; and analyzing and processing the transcriptome data corresponding to the site information according to the spatial transcriptome sequencing analysis result to obtain a spatial transcriptome data analysis result of the target sample slice at the site information. According to the method and the device, the technical problem that the reliability is relatively low as only a space transcriptome technology combined with single morphological information is adopted to analyze the sample in the related technology is solved.

Description

technical field [0001] The present invention relates to the technical field of data processing, and in particular, to a method and device for processing spatial transcriptome data, and a computer-readable storage medium. Background technique [0002] In organisms, cell location information is closely related to the exercise of function, so "spatial transcriptome technology" that preserves cell location information in tissue and simultaneously detects transcriptome information in tissue slices in microenvironment, development, cell migration, cell fate determination It has been widely used in research in related fields. At present, the 10X Visium Spatial Transcriptome Solution realizes unbiased detection of gene expression in tissue sections through the combination of histomorphology (H&E staining or immunofluorescence) and tissue in situ gene activity information. However, in the practical application requirements in the field of life sciences, histomorphological informatio...

Claims

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Application Information

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IPC IPC(8): G16B40/00G06T7/90
Inventor 刘国静张敏王艳华张莹侯子健赵云飞
Owner 天津诺禾致源生物信息科技有限公司
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