43results about How to "Reduce false negative results" patented technology

The invention discloses a primer and probe composition for detecting polymorphism of human CYP2C9 and VKORC1 genes, a kit and application. The primer and probe composition comprises three pairs of specific primers for amplifying CYP2C9*2, CYP2C9*3 and VKORC1 sites, and three specific fluorescent probes. The primers and the probes, disclosed by the invention, have high sensitivity, strong specificity and strong anti-interference capability; a manner combining an asymmetric PCR (Polymerase Chain Reaction) amplification and fluorescent probe melting curve analysis technologies is used for detecting the gene polymorphism; different gene types can be effectively distinguished according to the quantity and Tm value of a melting peak; a result is convenient, clear and objective to judge and read.Single-tube sampling can be used for simultaneously detecting 6 mutation types of 3 gene sites; the primer and probe composition is simple and convenient to operate and the detection efficiency is improved; a large batch of samples can be detected and clinical operation is facilitated.

The invention discloses a kit for detecting helicobacter pylori drug resistance gene polymorphism by a multiplex fluorescence PCR melting curve method. The helicobacter pylori drug resistance gene comprises the following three genes: a 23S rRNA gene, a 16S rRNA gene and a gyr A gene, the kit comprises a nucleic acid extraction reagent and a nucleic acid amplification reagent, the nucleic acid extraction reagent comprises superparamagnetic silicon oxide nano magnetic beads, a lysis solution, a washing solution and an eluent; the nucleic acid amplification reagent comprises a primer pair and a probe which respectively correspond to the helicobacter pylori drug resistance gene 23S rRNA gene, the helicobacter pylori 16S rRNA gene, the gyr A gene and an internal standard gene human housekeepinggene beta-globin. According to the invention, three drug-resistant sites and one internal standard gene can be simultaneously detected in a single tube, so that the detection flux of a sample is improved; meanwhile, interference between multiple pairs of primer probes in a detection system is improved, and the sensitivity and specificity of reagent detection are effectively improved.

The invention discloses a kit for EGFR gene mutation detection and application, and belongs to the fields of biotechnology and medicine. Compared with the prior art, the kit has the advantages that aprimer, a probe and the kit of an amplification system are high in detection sensitivity, and can detect specimens with the mutation content being smaller than 0.1 percent; the detection accuracy is high, a double control detection system and a locked nucleic acid technology are adopted, and the reliability of detection results is guaranteed; only 8 reactions are needed, including EGFR quality control reaction liquid, L858R detection reaction liquid, 19del detection reaction liquid, G719X detection reaction liquid, L861Q detection reaction liquid, 3Ins20 detection reaction liquid, T790M detection reaction liquid and S768I detection reaction liquid, to be used for detecting 29 common mutant types of EGFR gene in serum or plasma and tissue samples of non-small cell lung cancer patients.

The invention discloses a detection method for functional prophage as well as location and sequence thereof in bacteria. The detection method in the invention includes steps of: predicting an openingreading frame in the genome sequencing data of to-be-detected bacteria to obtain the proteins encoded by the opening reading frame, and comparing the protein sequence with the sequences in the proteinlibrary of the phage, wherein the proteins which are matched with the phage proteins are the functional proteins; searching a positive repeating sequence on the encoding genes of the functional proteins as well as upstream and downstream thereof, wherein a sequence between two positive repeating sequences is the candidate sequence of a candidate prophage; connecting the head and the tail of the candidate sequence, wherein the candidate prophage containing the sequencing read length crossing the head-to-tail connection of the candidate sequence is the functional phage, whereas the candidate prophage without the sequencing read length crossing the head-to-tail connection of the candidate sequence is not the functional phage. The method is simple in operation and has great application prospect.

The invention discloses an immunochromatography kit for jointly detecting IgM (Immunoglobulin M) and IgG (Immunoglobulin G) of multiple pathogens, the kit comprises an upper cover and a base which are clamped with each other, a sample groove is formed in the middle of the base, a plurality of test paper clamping grooves are formed along the radial direction of the sample groove, and the test paper clamping grooves are respectively communicated with the sample groove through a guide groove; a sample adding hole and an observation window are formed in the positions, corresponding to the sample groove and the test paper clamping groove, of the upper cover respectively, a test strip is clamped in each test paper clamping groove, each test strip is used for detecting one TORCH pathogen to be detected, and each test strip comprises a bottom lining. A sample pad, a first marker combination pad, a second marker combination pad, a reaction film and a water absorption pad which are tightly connected are sequentially attached to the upper portion of the bottom lining from front to back, the reaction film is sequentially coated with a first detection line, a second detection line and a quality control line from front to back, the first detection line is coated with a mouse anti-human IgG antibody, and the second detection line is coated with a mouse anti-human IgM antibody. According to the kit, multiple pathogens can be detected at the same time only through one-time sample adding, and co-detection of the IgM antibody and the IgG antibody is achieved.

The invention discloses a kit for BRAFV600E gene mutation. The kit comprises a PCR (Polymerase Chain Reaction) buffer solution, primer probe mixed liquor, an enzyme system 1, positive control and negative control. The primer probe mixed liquor comprises a mutation site gene and internal control primer probe, a quality control gene and internal control detection primer probe, a mutation and quality control probe 5' terminal marking FAM fluorescein, a 3' terminal marking MGB fluorescein, an internal control 5' terminal marking HEX fluorescein, and a 3' terminal marking BHQ1 fluorescein. Quality control gene selects a relatively conservative section of a human BRAF V600E gene, internal control selects a human beta-aCtin conservative gene, and a double control system is jointly used for monitoring a plasma sample DNA quality and PCR reaction process. Compared with the prior art, the kit utilizing the primer, the probes and an amplification system provided by the invention is high in detection sensitivity, and can be used for detecting a sample with the mutation content being 0.1 percent; the detection accuracy is high, a double-control detection system is adopted, and the reliability of a detection result is ensured.

The invention relates to a real-time fluorescent quantitative PCR (polymerase chain reaction) kit for one-step quantitative detection of KRECs gene and its application. The kit comprises a real-time fluorescent quantitative PCR reaction system based on real-time fluorescent PCR technique. The fluorescent PCR reaction system comprises forward and reverse primers specific to KRECs and beta-actin genes, and a specific fluorescent probe. The kit allows quick screening of neonatal immune system B-cell level, has high sensitivity and stability and provides excellent reproducibility, and this method is applicable to the quantitative detection of KRECs and to the functional screening of the neonatal immune system and is worthy of practical clinical application.

The invention discloses a colloidal gold chromatography test strip for rapidly detecting thyroid papillary carcinoma lymph node metastasis and a preparation method of the colloidal gold chromatography test strip. A sample pad, a combination pad, a nitrocellulose membrane and an absorption pad are sequentially overlapped at one end of a bottom plate from left to right; the binding pad is a polyester film sprayed with a Cyfra21-1/Braf V600E capture antibody-colloidal gold compound, the nitrocellulose film is coated with a detection line T and a quality control line C, an antibody in the detection line T is a Cyfra21-1/Braf V600E monoclonal antibody, and the quality control line C is goat anti-mouse IgG. The occurrence rate of false negative is reduced by improving the tissue utilization degree, the detection speed is increased through a chromatography test strip method, finally the purposes of reducing the missed diagnosis rate, relieving the disease burden of a patient and improving the working efficiency of thyroid surgery are achieved, and important clinical significance is achieved.

The invention provides a novel coronavirus detection kit and a detection method thereof, and belongs to the technical field of molecular biological detection. A series of primer probe groups for detecting the novel coronavirus are redesigned, an existing detection method is improved, and detection targets are further increased, so that the detection sensitivity of the novel coronavirus is improved, false negative results can be remarkably reduced especially for detection of low-copy-number samples, the sensitivity and the accuracy of detection are effectively improved, and high-efficiency, high-specificity and low-cost detection is realized. The kit can be used for qualitatively detecting the novel coronavirus genes in pneumonia suspected cases and suspected aggregated case patients infected by the novel coronavirus in vitro and samples such as nasopharyngeal swabs and sputum of other patients needing novel coronavirus infection diagnosis or differential diagnosis.

The present invention provides the PCR or RT-PCR method with unique prime and its application, and the unique primer is oligomeric nucleotide chain designed based on the template DNA chain order characteristic and has 3' end with 6-12 bases in complementary order with the template and 5' end flexibly designed. The method is superior to classical PCR method, which has one pair of primers and maybe negative effect on proliferation efficiency and the complementing between 3' end bases in forward and inverse primers. The present invention is suitable for use in the PCR or RT-PCR test of human and pathological microbe gene, in multiple PCR or RT-PCR to reduce the mutual effect between primers and as the inner reference reaction of other PCR or RT-PCR, and is favorable to obtaining quantitative or semi-quantitative results.

The invention provides a chromosome long segment insertion detection method and a long segment insertion detection method based on a MassARRAY platform, and relates to the technical field of biology.The detection method includes the steps: simultaneously identifying whether samples to be detected are wild and/or mutant by a first detection unit; identifying whether the samples to be detected aremutant or not by a second detection unit; reading and discriminating identification results of the first detection unit and identification results of the second detection unit; judging whether chromosome long segments are inserted into the samples to be detected or not. By the aid of the additional arranged second detection unit, chromosome long segment insertion can be effectively detected according to the identification results of the first detection unit and the second detection unit, false negative results are effectively decreased, and false positive results can be effectively decreased.Besides, the chromosome long segment insertion detection method has the advantage that types of the samples to be detected can be expanded.