Immunochromatography kit for jointly detecting IgM (Immunoglobulin M) and IgG (Immunoglobulin G) of multiple pathogens

Abstract

The invention discloses an immunochromatography kit for jointly detecting IgM (Immunoglobulin M) and IgG (Immunoglobulin G) of multiple pathogens, the kit comprises an upper cover and a base which are clamped with each other, a sample groove is formed in the middle of the base, a plurality of test paper clamping grooves are formed along the radial direction of the sample groove, and the test paper clamping grooves are respectively communicated with the sample groove through a guide groove; a sample adding hole and an observation window are formed in the positions, corresponding to the sample groove and the test paper clamping groove, of the upper cover respectively, a test strip is clamped in each test paper clamping groove, each test strip is used for detecting one TORCH pathogen to be detected, and each test strip comprises a bottom lining. A sample pad, a first marker combination pad, a second marker combination pad, a reaction film and a water absorption pad which are tightly connected are sequentially attached to the upper portion of the bottom lining from front to back, the reaction film is sequentially coated with a first detection line, a second detection line and a quality control line from front to back, the first detection line is coated with a mouse anti-human IgG antibody, and the second detection line is coated with a mouse anti-human IgM antibody. According to the kit, multiple pathogens can be detected at the same time only through one-time sample adding, and co-detection of the IgM antibody and the IgG antibody is achieved.

Description

technical field [0001] The invention relates to the technical field of immunological detection devices, in particular to an immunochromatographic kit for co-detection of IgM and IgG of multiple pathogens. Background technique [0002] TORCH pathogens are a group of pathogens that can cause neonatal malformation or abnormal development. TORCH infection refers to toxoplasma gondii (TOX), rubella virus (RV), cytomegalovirus (CMV), herpes simplex virus type I / II (HSV I / II), and other pathogens (Others), such as parvovirus B19, varicella-zoster virus, Coxsackie virus and other infections. TORCH infection is often occult. Due to the inconspicuous symptoms, most infected people are difficult to detect their own changes, but it has a greater impact on fetuses and newborns. Infection with these pathogens before or during pregnancy can lead to miscarriage, fetal arrest and deformities. [0003] At present, the clinical detection of TORCH infection mainly adopts the ELISA method, whi...

AI Technical Summary

Problems solved by technology

[0003] At present, the clinical detection of TORCH infection mainly adopts the ELISA method, which is cumbersome to operate and takes a long time to detect. The reagent does not require any equipment, and the result can be observed with the naked eye in a few minutes. It has been widely used in clinical testing.

The application of immunochromatography in the detection of TORCH antibodies mostly adopts a single joint detection. Since each test strip is relatively independent, it is necessary to add a sample separately to the sample area of ​​each test strip during detection, so Need to add samples multiple times, the operation is complicated and error-prone

In addition, the antibodies produced by TORCH infection include specific IgM antibody (acute infection) and IgG antibody (previous infection). If the two are tested separately on different test strips, the operation is cumbersome. Due to the competitive relationship between pathogen-specific IgM antibodies and IgG antibodies, false negative results may occur, affecting the accuracy of the results

Images