The invention discloses toothpaste for relaxing, moistening and refreshing. The toothpaste for relaxing, moistening and refreshing comprises the following components in parts by weight of 0-90 of a solvent, 1-90 of a hydrating agent, 0-90 of an abradant, 0.1-30 of a thickening agent, 0-20 of a foaming agent, 0-10 of a sweetening agent, 1-20 of a plant composite extract, 0.1-50 of an efficacy agentand 0.001-0.02 of probiotics. The invention further discloses a preparation method of the toothpaste for relaxing, moistening and refreshing. The toothpaste for relaxing, moistening and refreshing has the beneficial effects that the efficacy agent and an original abradant of toothpaste are in synergetic effect, the toothpaste has favorable effects of cleaning and whitening, and besides, has favorable effects of repairing and brightening. The plant composite extract and the probiotics are added, so that the toothpaste has durable and effective control on oral pathogenic bacteria of streptococcus sobrinus, porphyromonas gingivalis, streptococcus mutans, fusobacterium nucleatum and the like. Besides, the toothpaste has efficacy of resisting inflammation, can effectively assist in treating and preventing oral inflammation, can maintain oral smell to be fresh, can eliminate halitosis, has favorable feeling for relaxing, moistening and refreshing, has favorable repair effect on damage to human gum cells, and has favorable conservation efficacy of moisturizing oral cavity and the like.