The invention provides low-
nicotine paper-making process reconstituted tobacco and a preparation method and application thereof. The preparation method of the low-
nicotine paper-making process reconstituted tobacco comprises the following steps of: mixing tobacco fibers, wood pulp fibers and filler, then adding water, and performing heating extraction; centrifuging the leaching solution to obtaina tobacco extracting solution; pulping the
solid matter according to a
papermaking process, and forming to obtain a substrate; concentrating the tobacco extracting solution under reduced pressure to obtain water paste; adding
ammonia water into the water paste, performing uniform stirring, mixing with an
organic solvent, and performing extracting; separating the solution after extraction to obtainan organic phase and a water phase; mixing the water phase with an
ethanol solution, performing uniform stirring, and performing concentrating under reduced pressure to obtain a
coating solution; andcoating a substrate with the
coating solution, and performing
drying to obtain the low-
nicotine paper-making process reconstituted tobacco. The low-nicotine paper-making method reconstituted tobaccoprepared through adoption of the method is low in nicotine content, can replace part of tobacco raw materials to be added into traditional or novel cigarettes, can effectively reduce
tar, nicotine andother harmful substances in
smoke, and reduces harm to the
human body.