The invention discloses a kidney tonifying and hair blacking composition and application thereof. The kidney tonifying and hair blacking composition is prepared from 30-80 parts of radix rehmanniae preparata, 30-80 parts of rhizoma dioscoreae, 30-80 parts of semen cuscutae, 30-80 parts of walnut kernels, 20-40 parts of rhizoma polygonati, 20-40 parts of flatstem milkvetch seeds, 10-20 parts of cortex moutan, 10-20 parts of rhizoma alismatis, 10-20 parts of rhizoma gastrodiae, 10-30 parts of fructus corni, 5-15 parts of radix angelica sinensis, 5-15 parts of flos carthami, 5-15 parts of sargentgloryvine stems, 30-80 parts of radix polygoni multiflori preparata, 30-80 parts of black sesame seeds, 30-80 parts of black soya beans and 10-30 parts of chicken's gizzard-membrane. The application of the kidney tonifying and hair blacking composition to preparation of hair blacking drugs, hair blacking healthcare foods or hair blacking foods is also provided. Due to the fact that the prescription contains radix rehmanniae preparata, rhizoma dioscoreae, semen cuscutae, walnut kernels, rhizoma polygonati, flatstem milkvetch seeds, radix polygoni multiflori preparata, black sesame seeds, black soya beans and other kidney tonifying traditional Chinese medicines as well as liver tonifying traditional Chinese medicines like fructus corni and radix angelica sinensis, the purposes of tonifying kidney and liver and replenishing essence and blood can be realized, and the effect of blacking hair and beard is realized fundamentally.