The invention provides a dyeing and finishing method of a real silk / cotton intertexture, and belongs to a dyeing and finishing method of a fabric. The method comprises the following steps of: a, performing sewing and singeing treatment on a gray fabric; b, packaging and de-sizing; c, performing package and oxygen bleaching treatment, and drying; d, packaging and dyeing; e, packaging and softening; and f, performing preshrinking treatment to finish dyeing and finishing, wherein the warp and weft shrinkage rates of the fabric are less than 3 percent after the preshrinking treatment. Because biological de-sizing, scouring and degumming processes are adopted in the dyeing and finishing method, the method has the advantages of high efficiency and mild reaction conditions; particularly, because protease for degumming is adopted, powerful damage to real silk fibers is low, and the degumming effect is good; and by adopting a jig dyeing process, the quality problems of contamination, scratch, pleat and the like easily produced in a long-vehicle pad dyeing process can be effectively avoided.