The invention belongs to the technical field of medicine, and the invention discloses a deer melon polypeptide injection preparation; the preparation method of the injection preparation is as follows: the preparation method of deer bone extract is: take deer bone, add compound enzyme, extract with high temperature water, and adjust pH with acid value, heating, standing at 20-30°C, standing at 20-30°C after concentration, adjusting the pH value with alkali, standing at 20-30°C after concentration, adjusting the pH value with acid, gradient ultrafiltration, and storing at low temperature to obtain deer bone extract The preparation method of the melon seed extract is as follows: take the melon seeds and extract them with high-temperature water, adjust the pH value with acid, let it stand at 20-30°C, concentrate, centrifuge, ultrafilter, adjust the pH value with alkali, freeze and store at gradient temperature, and concentrate after thawing , centrifuged, pH value adjusted with acid after centrifugation, gradient ultrafiltration, low temperature preservation, to obtain the extract of melon seeds; after mixing the extract of deer bone and the extract of melon seeds, ultrafiltration to prepare an injection preparation.