Method for extracting galanthamine from lycoris plant

Abstract

The invention discloses a method for extracting galanthamine from a lycoris plant. The method comprises the following steps: (1) extraction: weighing the treated lycoris powder, adding the lycoris powder into water with pH of 4.5 at a feed-liquid ratio of 1:10 and a unit proportion of g / mL; adding the mixed enzyme of cellulase accounting for 1.0-3.0% of the mass of the lycoris powder and pectinase, wherein the mass ratio of the cellulase to pectinase is 1:1; performing enzymolysis for 20-100min at 50 DEG C; performing ultrasonic treatment for 5-25min and centrifuging for 20min at 5,000r / min; and fetching the supernate to obtain galanthamine extract; and (2) purification: solvent extraction; adsorption by cation exchange resin; C18 reverse phase silica gel column chromatography; normal-phase silica gel column chromatography; recrystallization: dissolving the dried product III obtained in the previous step with ethanol, crystallizing to obtain a crude product of galanthamine, filtering and performing vacuum drying at 50-60 DEG C to obtain galanthamine. The method disclosed by the invention has the advantages of easiness in operation, short extraction time, high extraction rate, small dosage of solvent and relatively low cost; and the purifying process is environment-friendly and the purity is high and can reach 99.2%.

Description

technical field [0001] The invention relates to a method for extracting galantamine, in particular to a method for extracting galantamine from plants of the genus Lycoris. Background technique [0002] The chemical synthesis of alkaloids such as galantamine in Lycoris has been reported, but extraction from Lycoris plants is still an important source of such substances. The extraction methods of Lycoris alkaloids in the prior art mainly include solvent Extraction, ultrasonic extraction, microwave extraction, supercritical fluid extraction and other methods, these methods generally have the disadvantages of serious environmental pollution and high production costs. Contents of the invention [0003] The technical problem to be solved by the present invention is to overcome the above-mentioned defects in the prior art and provide a method for extracting galantamine from Lycoris plants with short extraction time, low solvent consumption, high extraction rate and low cost . ...

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Problems solved by technology

[0002] The chemical synthesis of alkaloids such as galantamine in Lycoris has been reported, but extraction from Lycoris plants is still an important source of such substances. The extraction methods of Lycoris alkaloids in the prior art mainly include solvent Extraction method, ultrasonic extraction method, microwave extraction method, supercritical fluid extraction and other methods, these methods generally have the disadvantages of serious environmental pollution and high production cost.

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