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Method for extracting ganglioside from animal tissue

A ganglioside, animal tissue technology, applied in chemical instruments and methods, sugar derivatives, sugar derivatives and other directions, can solve the problems of high cholesterol content, high production cost, poor product purity, etc., and achieves simple production process, Yield and purity improvement, the effect of saving extraction time

Inactive Publication Date: 2003-03-05
上海宝智生物制品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The extraction process and extraction agent used in the methods described in the above documents and patents do not take into account that ganglioside is a highly polar acidic compound, which has such a basic feature as a strong negative charge. Therefore, the extraction The yield is low, the purity of the obtained product is poor, and the content of cholesterol, neutral lipids and other impurities is high, it must be refined repeatedly to meet the requirements of food and medicine, the production cost is high, and it cannot meet the needs of people. Demands in daily life, medicine and healthcare

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Grind 100 grams of pig brain tissue with a meat grinder, homogenate, soak three times in acetone in a cold place, use 3 times the amount for the first time, 2.5 times the amount for the second time, and 1.5 times the amount for the third time, each time 24 hours, filter with muslin or filter press;

[0035] Add 200ml of water and 1200ml of tetrahydrofuran to the above-mentioned processed pig brain tissue, stir well, centrifuge, and repeat the extraction once with 300ml of tetrahydrofuran for the residue, combine the secondary extracts, add 450ml of petroleum ether, perform multi-component distribution, and obtain the lower layer after layering. The polar solvent phase, the upper non-polar solvent phase was repeatedly extracted with 100ml of water, the lower polar solvent phase was combined, filtered, concentrated under reduced pressure at 45°C, and freeze-dried to obtain the finished product, which was grayish white, soluble in water, and soluble in chloroform— -Methano...

Embodiment 2

[0037] Grind and homogenate 500 grams of porcine brain tissue, dry it with hot air at 60°C, and grind it into a fine powder (moisture is less than 8%). Take the above-mentioned brain dry powder, soak it in acetone for 5 hours, repeat 5 times, filter, and put it on the filter cake Add 80% ethanol aqueous solution in the ratio of 1:5, stir fully for 1.5 hours, then add n-hexane according to 1:4, stir fully for 1.5 hours, after static layering, separate the ethanol solution in the lower layer, and use the upper non-polar phase Repeat extraction with 500ml of water, combine the alcohol and water extracts, concentrate under reduced pressure at 40°C, and freeze-dry to obtain the finished product, which is white or off-white with a purity of 79.0%.

Embodiment 3

[0039] 1 kg of bovine placenta, minced, homogenized, treated with acetone by the method of Example 1, added 2L of distilled water, 12L of tetrahydrofuran, fully stirred for 1.5 hours, centrifuged, and the residue was repeatedly extracted once with 3L of tetrahydrofuran. Combine the extracts, add 4.5L of n-hexane, carry out multiple partitioning for 1.5 hours, obtain the lower polar solvent phase after layering, and extract the upper non-polar solvent phase with 1L of distilled water once, combine the water phase twice, filter, and reduce pressure at 45°C Concentrated, freeze-dried. The finished product is white or off-white, with a purity of 80.5%.

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Abstract

The present invention discloses a method for extracting ganglioside from animal tissue. Said method adopts one kind of several kinds of polar solvents matched with polarity of ganglioside to greatly raise extraction efficiency of ganglioside, and adopts inversed phase extraction method to remove impurity from the extract to raise product grade and reduce production cost. The purity of the ganglioside prepared by using said invented method can be up to 81%, and its cholesterol and neutral lipide contents are low. Said invention is simple in production process, suitable for scale production, its product purity is high, and can meet the food and medicinal requirements.

Description

technical field [0001] The invention relates to a method for extracting glycolipid substances mainly composed of gangliosides from animal brain tissue and other tissues. Background technique [0002] Ganglioside is a membrane glycolipid containing N-acetylneuraminic acid (also known as sialic acid), which has the function of enhancing the development of human brain function and enhancing memory. Ganglioside is also a message in the nervous system Regulator, it has obvious curative effect and health effect in the aspects of treatment and biological protection (Annals of NewYork Academy of Science Volume 845 June 19.1998). For this reason, many scientific and technical personnel have carried out extensive research to the preparation method of ganglioside, as document: (Falch.J.etat.J.Bioll.Chem.1957226 (497)) and Chinese patent 951115693 namely reported respectively from A method for extracting glycolipid substances mainly composed of gangliosides from animal brain tissue. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H1/08C07H15/04C07H15/10
Inventor 冯万祥吴同新
Owner 上海宝智生物制品有限公司
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