A Lugua polypeptide injection
A technology for injection preparations and injections, which is applied in the field of medicine, can solve the problems that the preparation method is in question, and the content of ineffective components in the deer and melon polypeptide is not fully considered, and achieves the effects of reducing the number of extractions and improving the yield.
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Embodiment 1
[0076] Take the deer bone, remove the bone marrow, weigh 200kg, add 500kg of water, add 1kg of papain, 3kg of trypsin, store for 1 hour, extract at 121°C for 60 minutes, put the extract in a degreasing tank, let it stand, and skim off Float the fat to get the initial deer bone extract, add 1.5 mol / L hydrochloric acid solution to the deer bone initial extract, adjust the pH to 3.3, heat to 90°C, keep for 30 minutes, stand at 25°C for 16 hours, take the supernatant, discard The bottom precipitate was concentrated to 0.3 times the amount of deer bone, and stood at 25°C for 16 hours. The supernatant was taken, the bottom precipitate was discarded, and filtered with a 30 μm pretreatment column. The filtrate was adjusted to pH with 4.5 mol / L sodium hydroxide solution. 8.8, concentrated to 0.3 times the amount of deer bone, let stand at 25°C for 16 hours, take the supernatant, discard the bottom sediment, add 1.5 mol / L hydrochloric acid solution to the supernatant to adjust the pH val...
Embodiment 2
[0080] Take the deer bone, remove the bone marrow, weigh 200kg, add 500kg of water, add 0.60kg of papain, 2.40kg of trypsin, store for 1.5 hours, extract at 125°C, keep for 60 minutes, extract the liquid in a degreasing tank, and let it stand , Skim off the floating oil to get the initial deer bone extract, add 2.5 mol / L hydrochloric acid solution to the deer bone initial extract, adjust the pH to 3.5, heat to 80°C, keep for 34 minutes, stand at 20°C for 14 hours, take the supernatant , discard the precipitate at the bottom, concentrate to 0.25 times the amount of deer bone, let stand at 30°C for 13 hours, take the supernatant, discard the precipitate at the bottom, filter with a 30 μm pretreatment column, and adjust the filtrate with 5.5 mol / L sodium hydroxide solution When the pH value reaches 8.9, concentrate to 0.4 times the deer bone feeding amount, let stand at 28°C for 12.5 hours, take the supernatant, discard the bottom sediment, add 2.5 mol / L hydrochloric acid solution...
Embodiment 3
[0084] Take the deer bone, remove the bone marrow, weigh 200kg, add 400kg of water, add 1.42kg of papain, 3.58kg of trypsin, store for 2 hours, extract at 130°C, keep for 60 minutes, extract the liquid in a degreasing tank, and let it stand , Skim off the floating fat to get the initial deer bone extract, add 1 mol / L hydrochloric acid solution to the deer bone initial extract, adjust the pH to 3.0, heat to 100°C, keep for 25 minutes, stand at 20°C for 12 hours, take the supernatant , discard the bottom precipitate, concentrate to 0.2 times the amount of deer bone, let stand at 20°C for 12 hours, take the supernatant, discard the bottom precipitate, filter with a 30 μm pretreatment column, and adjust the filtrate with 5 mol / L sodium hydroxide solution When the pH value reaches 8.5, concentrate to 0.2 times the amount of deer bone, let it stand at 20°C for 12 hours, take the supernatant, discard the bottom sediment, add 1 mol / L hydrochloric acid solution to the supernatant to adj...
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