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Novel preparation method of coriolus versicolor polysaccharopeptide

A new technology of versicolor glycopeptide, which is applied in the field of preparation of new versicolor glycopeptide, can solve the problems of loss of active ingredients, long preparation time, loss of versicolor fungus powder, etc., and achieve improved yield and utilization rate and low water content , easy-to-operate effects

Pending Publication Date: 2021-07-23
JIANGSU SHENHUA PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

In this method, versicolor versicolor fungus powder will be lost in the process of processing, and the preparation time is long, and the active ingredients may be lost.

Method used

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  • Novel preparation method of coriolus versicolor polysaccharopeptide
  • Novel preparation method of coriolus versicolor polysaccharopeptide
  • Novel preparation method of coriolus versicolor polysaccharopeptide

Examples

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Embodiment 1

[0036] Example 1: Take 30L of the same batch of Versicolor versicolor fermented liquid, put it in a 50L fermenter, heat to 100°C, boil and extract for 2 hours, cool to room temperature, release the fermented liquid, centrifuge at 4000r / min for 5min, and separate the supernatant (fermentation filtrate) and wet mycelia precipitate (wet mycelium), extract the precipitate (wet mycelium) with 8 times the weight of water once, the extraction temperature is 100°C, the extraction time is 3h, the extract is centrifuged at 4000r / min for 5min, Collect the supernatant (mycelia extraction) and mycelium residue, add 5 times the weight of the mycelium residue to boil water for extraction for the second time, the extraction temperature is 100°C, and the extraction time is 2h; the extract is centrifuged at 4000r / min for 5min, and the mycelium The residue is discarded directly, the supernatant is collected, mixed with the supernatant (hyphae extraction) and the supernatant (fermentation filtrate...

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Abstract

The invention discloses a novel preparation method of coriolus versicolor polysaccharopeptide, which comprises the following steps of: directly heating and boiling coriolus versicolor fermentation broth in a fermentation tank, and directly extracting hyphae in the fermentation broth by using filtrate in the fermentation broth, so that the problem of loss of effective components in the hyphae possibly generated after the hyphae are separated and dried in the original process is avoided, a mixture of intracellular polysaccharide and extracellular polysaccharide of the coriolus versicolor can be obtained at the same time, and the yield and the utilization rate are improved. In addition, the method is simple in technological process and easy to operate.

Description

technical field [0001] The invention belongs to the technical field of biological extraction, and relates to a preparation method of a novel versicolor glycopeptide. Background technique [0002] Yunzhi glycopeptide is a high molecular glycopeptide polymer extracted from the mycelia of Polyporaceae plant Yunzhi. It has the function of replenishing essence, invigorating the spleen and nourishing the heart, and it also has a certain protective effect on cellular immune function and blood picture. For patients with esophageal cancer, gastric cancer and primary lung cancer who suffer from deficiency of Qi and Yin, heart and spleen deficiency caused by radiotherapy and chemotherapy. [0003] At present, the large-scale industrial production and registration process of Yunzhi glycopeptide are prepared separately. The intracellular sugar of Yunzhi is extracted by adding water to the Yunzhi fungus powder, the extracellular sugar of Yunzhi is prepared by the filtrate separated from...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/30C07K1/14C12P21/00C12R1/645
CPCC07K1/30C07K1/145C12P21/00
Inventor 曹宝臣袁峰王鹏陈文娟冯杰王燕
Owner JIANGSU SHENHUA PHARMA
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