Injection preparation prepared from beer bones and muskmelon seeds
An injection preparation and a technology of melon seeds, which are applied in the field of medicine, can solve the problems of not fully considering the content of ineffective components in the melon polypeptide, and the preparation method is to be discussed, so as to reduce the number of extractions and increase the yield.
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Embodiment 1
[0075] Take the deer bone, remove the bone marrow, weigh 200kg, add 500kg of water, add exonuclease and pepsin, of which exonuclease is 3.33kg, pepsin is 1.67kg, after storage for 1 hour, use 121°C to extract and keep the extract for 60 minutes In the degreasing tank, let it stand still, skim off the floating oil to obtain the initial deer bone extract, add 1.5 mol / L hydrochloric acid solution to the deer bone initial extract, adjust the pH value to 3.3, heat to 90°C, keep for 30 minutes, and stand at 25°C Set aside for 16 hours, take the supernatant, discard the bottom precipitate, concentrate to 0.3 times the amount of deer bone, let stand at 25°C for 16 hours, take the supernatant, discard the bottom precipitate, filter with a 30 μm pretreatment column, and filter the filtrate with 4.5 Mole / liter sodium hydroxide solution to adjust the pH value to 8.8, concentrate to 0.3 times the amount of deer bone, let it stand at 25°C for 16 hours, take the supernatant, discard the botto...
Embodiment 2
[0079]Take the deer bone, remove the bone marrow, weigh 200kg, add 500kg of water, add exonuclease and pepsin, including 2.5kg of exonuclease and 1.5kg of pepsin, store for 1.5 hours, extract at 125°C for 60 minutes, and extract Put the liquid in the degreasing tank, let it stand still, skim off the floating oil, and get the initial deer bone extract, add 2.5 mol / L hydrochloric acid solution to the deer bone initial extract, adjust the pH value to 3.5, heat to 80 ° C, keep for 34 minutes, 20 ° C Stand still for 14 hours, take the supernatant, discard the bottom precipitate, concentrate to 0.25 times the amount of deer bone, let stand at 30°C for 13 hours, take the supernatant, discard the bottom precipitate, filter with a 30 μm pretreatment column, and use the filtrate Adjust the pH value to 8.9 with 5.5 mol / L sodium hydroxide solution, concentrate to 0.4 times the amount of deer bone, let stand at 28°C for 12.5 hours, take the supernatant, discard the bottom precipitate, add 2...
Embodiment 3
[0083] Take the deer bone, remove the bone marrow, weigh 200kg, add 400kg of water, add 3.53kg of exonuclease and 2.47kg of pepsin, store it for 2 hours, extract it at 130°C, keep it for 60 minutes, and put the extract in a degreasing tank. Set aside, skim off the floating oil to obtain the primary deer bone extract, add 1 mol / L hydrochloric acid solution to the deer bone primary extract, adjust the pH value to 3.0, heat to 100°C, keep for 25 minutes, stand at 20°C for 12 hours, take the supernatant liquid, discard the bottom precipitate, concentrate to 0.2 times the amount of deer bone, let it stand at 20°C for 12 hours, take the supernatant, discard the bottom precipitate, filter with a 30 μm pretreatment column, and use 4 mol / L sodium hydroxide solution for the filtrate Adjust the pH to 8.5, concentrate to 0.2 times the amount of deer bone, let stand at 20°C for 12 hours, take the supernatant, discard the bottom sediment, add 1 mol / L hydrochloric acid solution to the superna...
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