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Injection preparation prepared from beer bones and muskmelon seeds

An injection preparation and a technology of melon seeds, which are applied in the field of medicine, can solve the problems of not fully considering the content of ineffective components in the melon polypeptide, and the preparation method is to be discussed, so as to reduce the number of extractions and increase the yield.

Inactive Publication Date: 2016-06-22
TIBET YUHENG YANGGUANG MEDICINE TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the above methods can obtain Lugua polypeptide injection, they do not fully consider the content of ineffective ingredients in Lugua polypeptide. Therefore, the above preparation method is open to discussion

Method used

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  • Injection preparation prepared from beer bones and muskmelon seeds
  • Injection preparation prepared from beer bones and muskmelon seeds
  • Injection preparation prepared from beer bones and muskmelon seeds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] Take the deer bone, remove the bone marrow, weigh 200kg, add 500kg of water, add exonuclease and pepsin, of which exonuclease is 3.33kg, pepsin is 1.67kg, after storage for 1 hour, use 121°C to extract and keep the extract for 60 minutes In the degreasing tank, let it stand still, skim off the floating oil to obtain the initial deer bone extract, add 1.5 mol / L hydrochloric acid solution to the deer bone initial extract, adjust the pH value to 3.3, heat to 90°C, keep for 30 minutes, and stand at 25°C Set aside for 16 hours, take the supernatant, discard the bottom precipitate, concentrate to 0.3 times the amount of deer bone, let stand at 25°C for 16 hours, take the supernatant, discard the bottom precipitate, filter with a 30 μm pretreatment column, and filter the filtrate with 4.5 Mole / liter sodium hydroxide solution to adjust the pH value to 8.8, concentrate to 0.3 times the amount of deer bone, let it stand at 25°C for 16 hours, take the supernatant, discard the botto...

Embodiment 2

[0079]Take the deer bone, remove the bone marrow, weigh 200kg, add 500kg of water, add exonuclease and pepsin, including 2.5kg of exonuclease and 1.5kg of pepsin, store for 1.5 hours, extract at 125°C for 60 minutes, and extract Put the liquid in the degreasing tank, let it stand still, skim off the floating oil, and get the initial deer bone extract, add 2.5 mol / L hydrochloric acid solution to the deer bone initial extract, adjust the pH value to 3.5, heat to 80 ° C, keep for 34 minutes, 20 ° C Stand still for 14 hours, take the supernatant, discard the bottom precipitate, concentrate to 0.25 times the amount of deer bone, let stand at 30°C for 13 hours, take the supernatant, discard the bottom precipitate, filter with a 30 μm pretreatment column, and use the filtrate Adjust the pH value to 8.9 with 5.5 mol / L sodium hydroxide solution, concentrate to 0.4 times the amount of deer bone, let stand at 28°C for 12.5 hours, take the supernatant, discard the bottom precipitate, add 2...

Embodiment 3

[0083] Take the deer bone, remove the bone marrow, weigh 200kg, add 400kg of water, add 3.53kg of exonuclease and 2.47kg of pepsin, store it for 2 hours, extract it at 130°C, keep it for 60 minutes, and put the extract in a degreasing tank. Set aside, skim off the floating oil to obtain the primary deer bone extract, add 1 mol / L hydrochloric acid solution to the deer bone primary extract, adjust the pH value to 3.0, heat to 100°C, keep for 25 minutes, stand at 20°C for 12 hours, take the supernatant liquid, discard the bottom precipitate, concentrate to 0.2 times the amount of deer bone, let it stand at 20°C for 12 hours, take the supernatant, discard the bottom precipitate, filter with a 30 μm pretreatment column, and use 4 mol / L sodium hydroxide solution for the filtrate Adjust the pH to 8.5, concentrate to 0.2 times the amount of deer bone, let stand at 20°C for 12 hours, take the supernatant, discard the bottom sediment, add 1 mol / L hydrochloric acid solution to the superna...

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Abstract

The invention belongs to the technical field of medicine and discloses an injection preparation made of deer bone and melon seeds as raw materials; the preparation method of the injection preparation is as follows: deer bone is extracted to obtain a deer bone extract, and melon seeds are extracted to obtain a melon seed extract; wherein The preparation method of deer bone extract is as follows: take deer bone, add compound enzyme, extract with high-temperature water, adjust pH value with acid, heat, stand at 20-30°C, concentrate at 20-30°C, adjust pH value with alkali, concentrate at 20°C Stand at -30°C, adjust the pH value with acid, gradient ultrafiltration, and store at low temperature to obtain the deer bone extract; the preparation method of the sweet melon seed extract is: take the melon seeds and extract with high temperature water, adjust the pH value with acid, and statically at 20-30°C After concentration, centrifugation, ultrafiltration, alkali adjustment of pH value, gradient temperature cryopreservation, concentration after thawing, centrifugation, acid adjustment of pH value after centrifugation, gradient ultrafiltration, low temperature storage, to obtain the melon seed extract; the deer bone extract and The extracts of melon seeds are mixed, ultrafiltered, and prepared into injection preparations.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to an injection preparation made of deer bone and melon seeds as raw materials. Background technique [0002] The pharmaceutical preparations made from deer bones and melon seeds are mainly deer melon polypeptide injection preparations, which can regulate bone metabolism, stimulate osteoblast proliferation, and promote new bone formation, and can also regulate calcium scale metabolism and increase bone calcium deposition , prevent osteoporosis, and has anti-inflammatory, analgesic, anti-rheumatic effects, and has been widely used clinically. Clinical application shows that the injection preparation made of deer bone and melon seeds is an effective drug for the treatment of nonunion of fracture, osteoporosis, rheumatism, rheumatoid arthritis, avascular necrosis of scaphoid bone and soft tissue injury, etc. The application prospect of orthopedics is broad. [0003] Chin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/01A61K9/08A61K9/19A61P19/08A61P19/10A61P29/00A61K35/32A61K36/42
Inventor 王国强程晓高
Owner TIBET YUHENG YANGGUANG MEDICINE TECH CO LTD
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