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Celery seed extract, preparation and preparation method thereof

A technology of celery seed extract and celery, which is applied in the direction of drug combination, plant raw materials, medical preparations of non-active ingredients, etc., can solve the problems of complicated extraction steps, difficult industrial production, and no refining method, so as to improve bioavailability The effect of high temperature, mild conditions and reasonable purification steps

Active Publication Date: 2017-03-08
赛隆药业集团股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing extraction methods of celery seed extract have many shortcomings such as complicated extraction steps, low crude drug rate, basically no refining method, poor repeatability, etc., and are difficult to industrialized production

Method used

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  • Celery seed extract, preparation and preparation method thereof
  • Celery seed extract, preparation and preparation method thereof
  • Celery seed extract, preparation and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A kind of celery seed extract is prepared by following steps:

[0031] a. Take dried celery seeds, crush them, add 2 times the volume of ethanol aqueous solution with a volume fraction of 95%, and reflux for extraction for 1.5 hours; collect the extract, concentrate under reduced pressure until it is free of alcohol, and obtain a thick extract;

[0032] b. the thick extract obtained in step a is suspended in water of 1 times the volume of the raw material, then extracted twice with a mixed solution of sherwood oil and ethyl acetate with a volume ratio of 3:1, and the amount of the extraction reagent added each time is the raw material 0.2 times the volume, after the extraction is completed, the organic phases are combined, dried, and concentrated under reduced pressure to obtain the celery seed extract;

Embodiment 2

[0034] A kind of celery seed extract is prepared by following steps:

[0035] a. Get dried celery seeds, pulverize, add 95% ethanol aqueous solution to reflux and extract 2 times, each time add ethanol aqueous solution to be 2.5 times of volume, reflux time is 0.5h, collect and combine extracts, concentrate under reduced pressure to No alcohol, get thick extract;

[0036] b. suspend the thick extract obtained in step a in water of 0.5 times the volume of the raw material, then extract 4 times with ethyl acetate or dichloromethane, and add the amount of extraction reagent each time to 0.4 times the volume of the raw material. After the extraction, combine the organic phase, dried, and concentrated under reduced pressure to obtain a crude extract;

[0037] c. Dissolve the crude extract in ethyl acetate at a concentration of 0.2 g / ml, and put it on a silica gel chromatography column;

[0038] d. Gradient elution: the elution flow rate is 7ml / min, first elute 10 column volumes w...

Embodiment 3

[0040] A preparation method for preparing celery seed extract, the preparation method steps are as follows:

[0041]a. Extraction: take the dried celery seeds, crush them, add 4 times the volume of ethanol aqueous solution with a volume fraction of 95% to reflux for extraction for 1 hour, and at the same time carry out ultrasonic assistance; collect the extract, concentrate it under reduced pressure until it is free of alcohol, and obtain a thick extract;

[0042] b. Extraction: suspend the thick extract obtained in step a in water of 0.9 times the volume of the raw material, then extract 3 times with a mixed solution of ethyl acetate and n-hexane with a mass ratio of 1:2, adding the amount of extraction reagent each time 0.3 times the volume of the raw material, after the extraction is completed, the organic phases are combined, dried, and concentrated under reduced pressure to obtain the celery seed extract;

[0043] c. Dissolve the crude extract in ethyl acetate at a concen...

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Abstract

The present invention relates to a celery seed extract, a preparation and a preparation method thereof. The preparation method comprises: taking dried celery seeds, and carrying out reflux extraction 1-2 times with 2-4 times the volume of an ethanol aqueous solution with a volume fraction of 95%; collecting the extraction liquids, and carrying out pressure reducing concentration to obtain a thick extract; suspending the thick extract in water having the volume 0.5-1 time the volume of the raw material, extracting 2-4 times with an extraction reagent, and combining the organic phases after completing the extracting to obtain a crude extract; weighing silica gel having the mass of 35-55 times the mass of the crude extract, activating for 1 h at a temperature of 130 DEG C, and loading into a chromatography column; taking the crude extract, and injecting in a concentration of 0.2-0.5 g / ml; and carrying out gradient elution with petroleum ether-dichloromethane-ethyl acetate, collecting the flow, and carrying out pressure reducing concentration to obtain the celery seed extract. According to the present invention, the preparation method has characteristics of simple preparation process, less extraction number, and high yield; and the obtained celery seed extract has the good serum uric acid lowering effect.

Description

technical field [0001] The invention belongs to the field of pharmaceutical preparations, in particular to a celery seed extract and its preparation and preparation method. Background technique [0002] Celery seed is the seed of Apium graveolens L., an umbrella plant. Celery seed has a history of 100 years in Australia for treating joint pain. It is regarded as a traditional folk remedy. Celery seed extract has the functions of lowering uric acid, lowering blood pressure, Blood lipids and other effects, the effect of lowering uric acid is the most significant. Most domestic researchers carry out relevant research and reports on the root, stem, leaf extract and celery seed essential oil of celery, but less related to the relevant research of celery seed extract; Chinese patent CN100569241C discloses a kind of celery Seed ethyl acetate extract, preparation method is as follows: Celery dry seed 10kg, with 15~20 times of amount 75~95% (V / V) ethanol percolation extraction or wi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/23A61K9/48A61K47/38A61P19/06A61P13/12A61K131/00
CPCA61K9/4816A61K9/4866A61K36/23A61K2236/55A61K2236/39A61K2236/35A61K2236/333
Inventor 李剑峰
Owner 赛隆药业集团股份有限公司
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