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38results about How to "Stable integration" patented technology

Combined adeno-associated virus and adenovirus cocktail gene delivery system for high efficiency gene expression without eliciting immune response in immuno-competent subjects

The present invention provides an efficient gene delivery system using Adeno-Associated Viral (AAV) vector in gene therapy. Furthermore, the invention provides a combined AAV and Adenovirus (Adv) cocktail gene delivery system which is even more efficient in in vivo gene delivery and expression without eliciting any significant immune responses in an immunocompetent subject. In particular, the invention provides a therapeutic agent and methods for preventing, treating, managing, or ameliorating various diseases and disorders including, but not limited to, bone diseases, by delivering Bone Morphogenetic Protein 2 (BMP-2) for new bone formation via gene therapy using said system. The invention provides a nucleic acid molecule comprising an AVV vector and a promoter operably linked to a sequence encoding BMP-2; and a nucleic acid molecule comprising an Adv vector and a promoter operably linked to a sequence encoding BMP-2, as well as vectors and host cells comprising said nucleic acid molecules, respectively.
Owner:THE UNIVERSITY OF HONG KONG

Combined compression and shearing process for the production of a crushed polyolefin foam and product obtained thereby

The present invention provides a method for producing a polyolefin-based resin crosslinked foamed sheet which is excellent in heat resistance and flexibility, has a small diameter of cells, can be developed in various utilities, and is excellent in vacuum moldability. The method for producing a polyolefin-based resin crosslinked foamed sheet according to the present invention comprises the steps of supplying a foamed sheet with closed cells, comprising a polyolefin-based resin, to a gap between one pair of rolls which have different circumferential speeds, and are rotated so that rotation directions on facing surfaces are the same direction, and applying a compression force and a shear stress to the foamed sheet with closed cells simultaneously to rupture a part of closed cells of the foamed sheet with closed cells to communicate closed cells into open cells.
Owner:SEKISUI CHEM CO LTD

Compound type piggyBac recombinant vector as well as preparation method and application of compound type piggyBac recombinant vector

InactiveCN104673815AIntegration and stabilityNo retranspositionVector-based foreign material introductionAnimal husbandryHeat shockFrame sequence
The invention discloses a compound type piggyBac recombinant vector as well as a preparation method and an application of the compound type piggyBac recombinant vector. According to the recombinant vector, a target gene expression frame, selection marker gene expression frames I and II, piggyBac transposase expression frame regulated by a heat shock promoter and truncated type piggyBac transposon arms L2 and R2 are inserted between wild type piggyBac transposon arms R1 and L1, integration of target gene, selection marker gene, piggyBac transposase expression frame sequence in silkworm genome can be conveniently mediated, complete deletion of all of transposon sequences and selection marker gene sequences in progeny transgenosis silkworm genome can be realized through establishing and screening a transgenosis silkworm line with high target gene expression and then inducing piggyBac to express in the transgenosis silkworm by heat shock treatment, replacement of other exogenous gene and the target gene can be conveniently realized through box type exchange reaction mediated by phiC31 integrase by surplus target gene expression frame anchored by attP locus, and then fixed point integration of other exogenous genes in the genome locus is further realized.
Owner:SOUTHWEST UNIVERSITY

Dental Ridge Augmentation Matrix with Integrated Dental Implant Surgical Drill Guide System

The subject matter of the instant invention relates to a dental implant surgical apparatus and system comprising a customized dental ridge augmentation matrix and one or, optionally, a plurality of dental implant surgical drill guides, and methods of use thereof.
Owner:THE UNITED STATES OF AMERICA AS REPRESENTED BY THE SECRETARY OF THE NAVY

Methods and compositions for inhibiting angiogenesis

The present invention provides a method of inhibiting angiogenesis within a tissue by providing exogenous PEDF to cells associated with the tissue. The presence of exogenous PEDF inhibits angiogenesis within the tissue, in part by interfering with the ability of vascular endothelia to expand within the tissue. The invention also provides a method for determining the severity of a tumor be assaying for the presence of PEDF within the tumor. To facilitate the inventive methods, the present invention provides pharmaceutical compositions including sources of PEDF.
Owner:NORTHWESTERN UNIV

Human blood coagulation factor IX mutant pichia pastoris expression vector and construction method and application thereof

The invention discloses a human blood coagulation factor IX mutant pichia pastoris expression vector and a construction method and application thereof. The procedures of the construction method of the human blood coagulation factor IX mutant pichia pastoris expression vector includes that according to a hFIX gene sequence, the reverse transcription-polymerase chain reaction (RT-PCR) technology is used for cloning hFIX overall length complementary deoxyribonucleic acid (cDNA) from human hepatic cells, signal peptide mixed with yeast Alpha -factor and yeast expression plasmids pPIC9K-hFIX are constructed to express pichia pastoris, and then based on that the yeast expression plasmids pPICZ Alpha A-hFIX are constructed, four saccharomycetes hFIX high-activity mutants are constructed. Cruor activity of the achieved hFIX yeast expression vector is apparently higher than that of standard hFIX. One of the mutants is through 50-litre pilot scale test of fermentation, protein purification product secretory expression quantity is 702 milligrams / litre, so that a good foundation is laid for producing efficient hFIX products used for treating hemophilia B in a low cost and industrialized mode.
Owner:WUHAN UNIV OF SCI & TECH

Transgene method for peanut

The invention discloses a process for transgene of peanuts, of which the steps include that initially repetitive somatic embryogenesis culture of the peanuts is established, then exogenous DNA or genes are constructed in a plant expression frame or an expression vector and the concentration of the exogenous DNA is extracted, purified and identified, thirdly the repetitive somatic embryogenesis culture is transformed via micro-projectile bombardment and independent resistant cell lines are screened, fourthly, plant regeneration and passage happens, and fifthly transgenic plant and transgenic molecules in the progenies is analyzed. The invention employs the repetitive somatic embryogenesis culture after which is started to be cultured for 1 to 10 months and is induced by mature peanut seeds which are stored for 0 to 6 years as a transgenic acceptor. The transgenic efficiency is high and the transformation flux is high, and the screen efficiency after the bombardment is high without escaping and chimeras. Genotype dependence does not exist in the whole process, the regeneration of the plant is easy and highly effective, transgenic descendant seeds can be abundantly obtained, and the exogenous gene is capable of stably conforming and expressing inheritance.
Owner:INST OF SUBTROPICAL AGRI CHINESE ACAD OF SCI +1

Eyedrop bottle sorting and casing system and method

The invention relates to an eyedrop bottle sorting and casing system device through a parallel four-axis robot provided with a visual system. The device comprises a working platform. A robot module, avisual system module and a workpiece platform module are arranged on the working platform. The robot module comprises the four-axis parallel robot and a control cabinet. The workpiece platform modulecomprises a photographing zone platform, a conveying belt, a charging zone and a discharging zone. The visual system module comprises a 2D camera, a light source, a computer and a visual software system. Specific to different gestures of eyedrop bottles in a detection zone, grabbing of different types is conducted, and a robot gas claw grabs the eyedrops and then sorts and cases the eyedrops. Inthe advanced manufacturing industry, the machine visual precision can be improved, the working efficiency can be improved, and the production cost is reduced.
Owner:GUANGDONG UNIV OF TECH

Genetic engineering of drought tolerance via a plastid genome

This invention provides a method of conferring osmoprotection to plants. Plant plastid genomes, particularly the chloroplast genome, is transformed to express an osmoprotectant. The transgenic plants and their progeny display drought resistance. More importantly, such transgenic plants display no negative pleiotropic effects such as sterility or stunted growth.
Owner:UNIV OF CENT FLORIDA +1

Recombinant CHO cell strain capable of stably expressing GPC3 and application thereof

The invention discloses a recombinant CHO cell strain capable of stably expressing GPC3 and an application thereof. The preservation number of the CHO cell strain is CGMCC No.18881, the GPC3 is encoded by a cDNA sequence shown in the formula of SEQ ID No. 1, an antigen produced by the recombinant CHO cell strain is used for preparing a GPC3 monoclonal antibody, and the recombinant CHO cell strainis used for preparing a GPC3 antibody. The folate reductase defect type CHO cells are lack of endogenous dihydrofolate reductase, when an expression vector containing a dihydrofolate reductase gene and a target gene is co-transfected, the dihydrofolate reductase gene and the target gene are generally integrated at the same site of a host chromosome, and then in the presence of a competitive inhibitor-methotrexate of dihydrofolate reductase, the increasing of the copy number of the dhfr gene is driven to achieve the purpose of increasing the expression quantity of the target gene, so that a stable cell line for stably and efficiently expressing GPC3 is constructed, and the technical blank is filled.
Owner:南京拂晓生物科技有限公司

Continuous rotation electrolysis method and device for lead solution

The invention discloses a continuous rotation electrolysis method and device for a lead solution and belongs to the field of electrolytic refining of lead. According to the continuous rotation electrolysis method and device for the lead solution, continuous electrolysis is performed on the lead solution; the matched device comprises an electrolysis tank and electrode plates; each electrode plate is round; the positive electrode is fixed, and the negative electrode rotates; and the two sides of the negative electrode plate are each provided with a scraper, and the scrapers are matched with a chute. The solution containing lead enters the electrolysis tank, lead ions are reduced into metal lead on the negative electrode, precipitated lead is scraped down by the scrapers and falls into the chute when rotating to the scrapers along with the negative electrode plate, then other subsequent links that the precipitated lead is collected to a conveying belt along the chute are performed, and gas is released on the positive electrode, and is exhausted from an exhaust port in the top of the electrolysis tank. According to the continuous rotation electrolysis method and device for the lead solution, continuous feeding and continuous discharging are performed in the electrolysis process of the lead solution, maintenance is convenient, the negative electrode plate rotates and also uniformly stirs electrolyte, the lead concentration gradient of the electrolyte is reduced, and the electrolysis efficiency is improved. The device is stable in single-machine treatment capacity, multi-machine parallel connection can also be performed according to the production requirement so as to improve the productivity, and stabilization can also be kept.
Owner:XIANGTAN UNIV

Kitchen device with suction foot

InactiveUS20160345781A1Stable integrationPrevents loose movementKitchen equipmentSuction cupsEngineeringActuator
The invention relates to a kitchen device (100) comprising: a grip (120) for manually holding the device, the grip defining a grip area (112), a first suction foot (130) attached to the device (100) and arranged to secure the device (100) to a surface (150) by 100 means of suction against the surface (150), an actuator (110) connected to the first suction foot (130) and extending in the grip area (112), the actuator (110) being arranged to transfer an actuating force (111) acting on the actuator (110) into a releasing force (131) acting on the first suction foot (130) to release the first suction foot (130) from the surface (150). This device can be released from the surface with an improved ease of use, because holding the device and releasing the device from the surface effectively have become a single, intuitive manual action by the user.
Owner:KONINKLJIJKE PHILIPS NV

Micron-nanometer structural bionic scaffold and preparation method

The invention provides a micron-nanometer structural bionic scaffold. Through the application of self-assembled collagen nano-fibers and bioactive glass nano-particles, strengthening can be performedon a scaffold respectively from the pores and the skeleton of a micron-sized silk fibroin porous scaffold, so that the micron-nanometer structural bionic scaffold strengthened on both inside and outside can be built. Through the discovery of experiments, the obtained bionic scaffold can have excellent mechanical properties, the collagen nano-fibers, the bioactive glass and silk fibroin can be stably integrated, and the osteogenesis of stem cells can be better promoted through the bionic structure of the bionic scaffold.
Owner:THE FIRST AFFILIATED HOSPITAL OF SOOCHOW UNIV

Wild type and mutant hFIX (human coagulation factor IX) Pichia pastoris expression vectors, and construction method and application thereof

The invention discloses a construction method and application of expression vectors of a wild type hFIX (human coagulation factor IX) and three hFIX mutants in Pichia pastoris. The construction method comprises the following steps: according to a gene sequence provided by Genebank, cloning a hFIX full-length cDNA (complementary deoxyribonucleic acid) sequence from healthy human liver through a RT-PCR (reverse transcription-polymerase chain reaction) technology, constructing a yeast expression plasmid pPIC9K-hFIX which is used for Pichia pastoris expression and is fused with a yeast alpha-factor signal peptide and a full-length hFIX sequence, transferring the plasmid into Pichia pastoris SMD1168, and screening out a high-level expressing yeast strain; and then, constructing a yeast expression plasmid pPICAZ-hFIX, designing and constructing three yeast hFIX high-activity mutants on this basis, electrically transferring into Pichia pastoris SMD1168, and screening out high-level expressing yeast mutant strains. The coagulation activity of each of the obtained various hFIX yeast expression vectors is higher than that of a standard hFIX. The 50L pilot fermentation research on the wild type hFIX (pPIC9K-hFIX) shows that the maximum protein purification product secretion expression quantity can be up to 558 mg / L and the purity and coagulation activity are high.
Owner:WUHAN UNIV OF SCI & TECH

Compressor pulley assembly and method for manufacturing same

Disclosed therein are a pulley assembly for a compressor and a manufacturing method of the same, which can prevent possible damages during processing and is fit for high-temperature environment because a pulley is made of magnesium alloy. The pulley assembly includes: a cylindrical hollow pulley made of magnesium alloy; an inner ring formed integrally with the pulley by insert injection molding, the inner ring being fixed to the inner circumferential surface of the pulley and made of a different material from the pulley; and a bearing forcedly pressed and fixed to the inner circumferential surface of the inner ring.
Owner:HANON SYST

Orotate transporter encoding maker genes

A recombinant marker gene encoding an orotate transporter polypeptide comprising an amino acid sequence at least 60% identical to SEQ ID NO: 2, a polynucleotide construct comprising at least one copy of the recombinant marker gene, a cell comprising at least one exogenous copy of the marker gene, and a method of selecting or identifying a cell comprising at least one copy of the recombinant marker gene, and / or selecting or identifying a cell which has been cured of the recombinant marker gene.
Owner:NOVOZYMES AS

Construction and application of novel angiotensin converting enzyme inhibitor screening model

The invention relates to construction and application of a novel angiotensin converting enzyme inhibitor screening model, and belongs to the technical field of biotechnology and food development. Specifically, the C structural domain protein of an angiotensin converting enzyme is successfully expressed by PCR, plasmid construction, protein extraction and the like, a stable liposome simulated membrane is obtained by combining repeated freezing and thawing with liquid nitrogen and extrusion with a liposome extruder, and finally the angiotensin converting enzyme bound with the simulated membraneis obtained; the enzyme activity of the system is measured by high-performance liquid chromatography, and it is verified that the model has practical significance. The constructed screening model canbe specifically used for exploring the functional rule of angiotensin converting enzyme inhibitors in membrane surface environments and is expected to provide a new idea for screening the angiotensinconverting enzyme inhibitors; meanwhile, the problem of large difference between in-vivo activity and in-vitro activity in screening of the angiotensin-converting enzyme inhibitors can be better solved.
Owner:JILIN UNIV

Construction and application of universal carrier for performing heterologous protein expression on pichia pastoris and hansenula polymorpha

The invention relates to universal carriers constructed based on methylotrophic hansenula polymorpha and pichia pastoris, in partituclar to a construction method of a universal carrier for performing heterologous protein expression on pichia pastoris and hansenula polymorpha. The construction method comprises the following steps that (1) a plasmid pHanMOX-GFP is digested through restriction enzymes Bgl II and BamH I, so that a pHan carrier is obtained; (2) a carrier plasmid pPinkAOX1-HPV16L1 is digested through restriction enzymes Bgl II and BamH I, a fragment PAOX1-HPV16L1-CYC1TT is recovered and then connected with the pHan carrier obtained in the step (1) through a T4 ligase at 16 DEG C overnight, an LB flat plate containing 0.1 mg / mL kanamycin is converted and coated, and plasmid enzyme digestion is extracted for identification, so that a restructured hansenula polymorpha plasmid pHanAOX1-HPV16L1 is obtained. The universal carrier is used for guiding expression of human papillomavirus type 16 major capsid protein L1 in pichia pastoris and hansenula polymorpha.
Owner:INST OF MEDICAL BIOLOGY CHINESE ACAD OF MEDICAL SCI

Method and device for continuous electrolysis of lead solution for hydraulic unloading of lead

The invention discloses a method for lead solution continuous electrolysis of hydraulic power lead discharging and a device for implementing the method and belongs to the field of electrolytic refining of lead. Continuous electrolysis is performed on a lead solution, specifically, the lead solution is continuously conveyed into an electrolytic bath, lead obtained through electrolysis is continuously conveyed out of the electrolytic bath, electrolyte in the electrolytic bath circularly flows through a pump, and lead is separated out at a negative electrode, flushed through the flowing electrolyte to drop to the bottom of the electrolytic bath and then conveyed into a lead collecting tank through a spiral part. The device comprises the electrolytic bath and electrolytic electrode plates, wherein the electrolytic electrode plates are rectangular; both the negative electrode plate and the positive electrode plate are fixed; baffles are disposed on the two sides of the negative electrode plate; the electrolytic bath is externally connected with the circulating pump which makes the electrolyte in the electrolytic bath to circularly flow; and the bottom of the electrolytic bath is conical and is connected with the lead collecting tank through the spiral part. According to the method and the device, continuous feeding and continuous discharging are achieved in the lead solution electrolysis process, maintenance is convenient, the electrolyte circularly flows to achieve complete homogenization, the lead concentration gradient of the electrolyte is reduced, and the electrolytic efficiency is improved.
Owner:XIANGTAN UNIV

Vitro method for high throughput screening of genotoxic agents in eukaryotic cells

The present invention relates to a novel method for high throughput detection of wide range of genotoxins in eukaryotic cells wherein the eukaryotic cell based tool combines the ability to detect a broad spectrum of genotoxic signaling events and a simple and reproducible assay technique. The present invention further comprises expression cassettes, vectors, and eukaryotic cell lines for the same.
Owner:ANTHEM BIOSCI PVT

Vitro method for high throughput screening of genotoxic agents in eukaryotic cells

The present invention relates to a novel method for high throughput detection of wide range of genotoxins in eukaryotic cells wherein the eukaryotic cell based tool combines the ability to detect a broad spectrum of genotoxic signaling events and a simple and reproducible assay technique. The present invention further comprises expression cassettes, vectors, and eukaryotic cell lines for the same.
Owner:ANTHEM BIOSCI PVT

T cell receptor library

The invention relates to a library for the expression of all functional TCR types comprising 45 TCR constructs each encoding one of the 45 different TCR α chains and 47 TCR constructs each encoding one of the 47 different TCR β chains, wherein each of the 45 TCR constructs encoding one of 45 different TCR α chain comprises the following building blocks one of the variable AV segments AVseg1 to AVseg45, and a constant AC segment, and wherein each of the 47 TCR constructs encoding one of 47 different TCR β chains comprises one of the variable BV segments BVseg1 to BVseg47, and a constant BC segment.
Owner:MEDIGENE IMMUNOTHERAPIES GMBH

Method for specificity overexpression of miRNA of slow virus mediated mammal cells

The invention relates to a method for specificity overexpression of miRNA of slow virus mediated mammal cells. The method aims at solving the problem that an existing miRNA expression system cannot achieve specificity expression of single miRNA of an miRNA complementary pair. By means of an shRNA system, an miRNA mature sequence is adopted as an upstream sense sequence, an antisense sequence complementing the miRNA mature sequence at the downstream is mutated according to T to C and A to G rules, the mutated RNA second-level neck ring structure is cloned to a carrier, then virus packaging is performed, and a cell line of the RNA second-level neck ring structure obtained after stable conversion mutation is built. One specific miRNA in the complementary miRNA pair can be overexpressed, and the method is applied to the field of miRNA overexpression.
Owner:HARBIN INST OF TECH

Battery production method and battery production device

The present invention provides a battery production method and a battery production device capable of decreasing the occurrence of defects, the method being characterized by: a first step of compressing predetermined regions from a plurality of collector foils (14) superposed onto each other and exposed at an end of an electrode (12), thereby forming a compressed region (16) wherein the interval between adjacent collector foils (14) has been reduced; and a second step of joining by ultrasound welding the regions of the plurality of collector foils (14) inside the compressed region (16), thereby forming a joined region (18) wherein the plurality of collector foils (14) have been turned into a single body.
Owner:JET
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