38results about How to "Stable integration" patented technology

There is provided a method of manufacturing a shielded connector which includes: a terminal-attached wire; a housing containing the terminal of the terminal-attached wire; a shielded shell integrated to the housing; a knitted conductor mounted to the wire; and a shield ring interposing the knitted conductor between the shield ring and the shielded shell so that the knitted conductor is electrically connected to the shielded shell. The method includes: inserting, into the shielded shell, a single core having a stepped portion formed in a opposed drawing direction; bringing a contact portion formed at the shielded shell into contact with the core; covering the shield ring on the shielded shell via the knitted conductor; and calking the shield ring, the knitted conductor, and the shielded shell from outer side of the shield ring to form, on the shielded shell, a calk-deformed portion contained into the stepped portion of the core.

The subject matter of the instant invention relates to a dental implant surgical apparatus and system comprising a customized dental ridge augmentation matrix and one or, optionally, a plurality of dental implant surgical drill guides, and methods of use thereof.

The present invention provides a method of inhibiting angiogenesis within a tissue by providing exogenous PEDF to cells associated with the tissue. The presence of exogenous PEDF inhibits angiogenesis within the tissue, in part by interfering with the ability of vascular endothelia to expand within the tissue. The invention also provides a method for determining the severity of a tumor be assaying for the presence of PEDF within the tumor. To facilitate the inventive methods, the present invention provides pharmaceutical compositions including sources of PEDF.

The invention discloses a human blood coagulation factor IX mutant pichia pastoris expression vector and a construction method and application thereof. The procedures of the construction method of the human blood coagulation factor IX mutant pichia pastoris expression vector includes that according to a hFIX gene sequence, the reverse transcription-polymerase chain reaction (RT-PCR) technology is used for cloning hFIX overall length complementary deoxyribonucleic acid (cDNA) from human hepatic cells, signal peptide mixed with yeast Alpha -factor and yeast expression plasmids pPIC9K-hFIX are constructed to express pichia pastoris, and then based on that the yeast expression plasmids pPICZ Alpha A-hFIX are constructed, four saccharomycetes hFIX high-activity mutants are constructed. Cruor activity of the achieved hFIX yeast expression vector is apparently higher than that of standard hFIX. One of the mutants is through 50-litre pilot scale test of fermentation, protein purification product secretory expression quantity is 702 milligrams / litre, so that a good foundation is laid for producing efficient hFIX products used for treating hemophilia B in a low cost and industrialized mode.

The invention discloses a process for transgene of peanuts, of which the steps include that initially repetitive somatic embryogenesis culture of the peanuts is established, then exogenous DNA or genes are constructed in a plant expression frame or an expression vector and the concentration of the exogenous DNA is extracted, purified and identified, thirdly the repetitive somatic embryogenesis culture is transformed via micro-projectile bombardment and independent resistant cell lines are screened, fourthly, plant regeneration and passage happens, and fifthly transgenic plant and transgenic molecules in the progenies is analyzed. The invention employs the repetitive somatic embryogenesis culture after which is started to be cultured for 1 to 10 months and is induced by mature peanut seeds which are stored for 0 to 6 years as a transgenic acceptor. The transgenic efficiency is high and the transformation flux is high, and the screen efficiency after the bombardment is high without escaping and chimeras. Genotype dependence does not exist in the whole process, the regeneration of the plant is easy and highly effective, transgenic descendant seeds can be abundantly obtained, and the exogenous gene is capable of stably conforming and expressing inheritance.

The invention discloses a recombinant CHO cell strain capable of stably expressing GPC3 and an application thereof. The preservation number of the CHO cell strain is CGMCC No.18881, the GPC3 is encoded by a cDNA sequence shown in the formula of SEQ ID No. 1, an antigen produced by the recombinant CHO cell strain is used for preparing a GPC3 monoclonal antibody, and the recombinant CHO cell strainis used for preparing a GPC3 antibody. The folate reductase defect type CHO cells are lack of endogenous dihydrofolate reductase, when an expression vector containing a dihydrofolate reductase gene and a target gene is co-transfected, the dihydrofolate reductase gene and the target gene are generally integrated at the same site of a host chromosome, and then in the presence of a competitive inhibitor-methotrexate of dihydrofolate reductase, the increasing of the copy number of the dhfr gene is driven to achieve the purpose of increasing the expression quantity of the target gene, so that a stable cell line for stably and efficiently expressing GPC3 is constructed, and the technical blank is filled.

The invention discloses a method and a device for continuous rotating electrolysis of lead solution, belonging to the field of electrolytic refining of lead. The invention continuously electrolyzes the lead solution, and the supporting device includes an electrolytic cell and an electrode plate, the electrolysis plate is circular, the anode is fixed, the cathode rotates, scrapers are arranged on both sides of the cathode plate, and the scraper is matched with the chute. The lead-containing solution enters the electrolytic cell, and the lead ions are reduced to metallic lead at the cathode. The precipitated lead is scraped off by the scraper when the cathode plate rotates to the scraper, falls into the chute, and then collects along the chute to the conveyor belt and other subsequent links. The gas is released and exits from the exhaust port at the top of the electrolyzer. The present invention continuously feeds and discharges materials during the electrolysis of lead solution, and is easy to maintain. The rotation of the cathode plate also stirs and homogenizes the electrolyte, which reduces the lead concentration gradient of the electrolyte and improves the electrolysis efficiency. The device of the present invention is a single machine The processing capacity is stable, and the production capacity can also be increased by parallel connection of multiple machines according to the production demand, which can also remain stable.

This invention provides a method of conferring osmoprotection to plants. Plant plastid genomes, particularly the chloroplast genome, is transformed to express an osmoprotectant. The transgenic plants and their progeny display drought resistance. More importantly, such transgenic plants display no negative pleiotropic effects such as sterility or stunted growth.

The present invention provides a new semi-stable packaging cell line and a method to produce lentiviral vectors (LV), using the semi-stable packaging cell line. New methods and packaging cell lines of the invention are generated using a baculo-AAV hybrid system for stable expression of structural and regulatory lentiviral proteins, such system comprising a baculoviral backbone containing an integration cassette flanked by AAV ITRs, in combination with a plasmid encoding rep protein. This system allows to obtain a stable integration of the structural and regulatory HIV-1 proteins gag / pol and rev. The system allows to obtain a cell line including the structural and regulatory HIV proteins gag / pol and rev, to be used for a semi-stable LV production.

The invention discloses a method for lead solution continuous electrolysis of hydraulic power lead discharging and a device for implementing the method and belongs to the field of electrolytic refining of lead. Continuous electrolysis is performed on a lead solution, specifically, the lead solution is continuously conveyed into an electrolytic bath, lead obtained through electrolysis is continuously conveyed out of the electrolytic bath, electrolyte in the electrolytic bath circularly flows through a pump, and lead is separated out at a negative electrode, flushed through the flowing electrolyte to drop to the bottom of the electrolytic bath and then conveyed into a lead collecting tank through a spiral part. The device comprises the electrolytic bath and electrolytic electrode plates, wherein the electrolytic electrode plates are rectangular; both the negative electrode plate and the positive electrode plate are fixed; baffles are disposed on the two sides of the negative electrode plate; the electrolytic bath is externally connected with the circulating pump which makes the electrolyte in the electrolytic bath to circularly flow; and the bottom of the electrolytic bath is conical and is connected with the lead collecting tank through the spiral part. According to the method and the device, continuous feeding and continuous discharging are achieved in the lead solution electrolysis process, maintenance is convenient, the electrolyte circularly flows to achieve complete homogenization, the lead concentration gradient of the electrolyte is reduced, and the electrolytic efficiency is improved.

The present invention relates to a novel method for high throughput detection of wide range of genotoxins in eukaryotic cells wherein the eukaryotic cell based tool combines the ability to detect a broad spectrum of genotoxic signaling events and a simple and reproducible assay technique. The present invention further comprises expression cassettes, vectors, and eukaryotic cell lines for the same.

The invention relates to a kitchen device (100) comprising: —a grip (120) for manually holding the device, the grip defining a grip area (112), —a first suction foot (130) attached to the device (100) and arranged to secure the device (100) to a surface (150) by means of suction against the surface (150), —an actuator (110) connected to the first suction foot (130) and extending in the grip area (112), the actuator (110) being arranged to transfer an actuating force (111) acting on the actuator (110) into a releasing force (131) acting on the first suction foot (130) to release the first suction foot (130) from the surface (150). This device can be released from the surface with an improved ease of use, because holding the device and releasing the device from the surface effectively have become a single, intuitive manual action by the user.

The invention relates to a library for the expression of all functional TCR types comprising 45 TCR constructs each encoding one of the 45 different TCR α chains and 47 TCR constructs each encoding one of the 47 different TCR β chains, wherein each of the 45 TCR constructs encoding one of 45 different TCR α chain comprises the following building blocks one of the variable AV segments AVseg1 to AVseg45, and a constant AC segment, and wherein each of the 47 TCR constructs encoding one of 47 different TCR β chains comprises one of the variable BV segments BVseg1 to BVseg47, and a constant BC segment.

The present invention provides a battery production method and a battery production device capable of decreasing the occurrence of defects, the method being characterized by: a first step of compressing predetermined regions from a plurality of collector foils (14) superposed onto each other and exposed at an end of an electrode (12), thereby forming a compressed region (16) wherein the interval between adjacent collector foils (14) has been reduced; and a second step of joining by ultrasound welding the regions of the plurality of collector foils (14) inside the compressed region (16), thereby forming a joined region (18) wherein the plurality of collector foils (14) have been turned into a single body.