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Recombinant CHO cell strain capable of stably expressing GPC3 and application thereof

A technology of stable expression and cell line, applied in the field of recombinant CHO cell line, can solve the problems of high recurrence rate and lost treatment opportunity, and achieve the effect of easy large-scale culture

Active Publication Date: 2020-04-21
南京拂晓生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most patients have lost the best opportunity for treatment when they see a doctor, and only 10-20% of them can be surgically resected early. Even with surgical resection, the recurrence rate is still high

Method used

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  • Recombinant CHO cell strain capable of stably expressing GPC3 and application thereof
  • Recombinant CHO cell strain capable of stably expressing GPC3 and application thereof
  • Recombinant CHO cell strain capable of stably expressing GPC3 and application thereof

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Embodiment Construction

[0029] In order to make the technical means, innovative features, goals and effects achieved by the present invention easy to understand, the present invention will be further described below.

[0030] The examples described here are specific specific implementations of the present invention, and are used to illustrate the concept of the present invention. They are all explanatory and exemplary, and should not be construed as limiting the implementation of the present invention and the scope of the present invention. In addition to the embodiments described here, those skilled in the art can also adopt other obvious technical solutions based on the claims of the application and the contents disclosed in the description, and these technical solutions include adopting any obvious changes made to the embodiments described here. Replacement and modified technical solutions.

[0031] The specific steps of a recombinant CHO cell line stably expressing GPC3 are as follows:

[0032] ...

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Abstract

The invention discloses a recombinant CHO cell strain capable of stably expressing GPC3 and an application thereof. The preservation number of the CHO cell strain is CGMCC No.18881, the GPC3 is encoded by a cDNA sequence shown in the formula of SEQ ID No. 1, an antigen produced by the recombinant CHO cell strain is used for preparing a GPC3 monoclonal antibody, and the recombinant CHO cell strainis used for preparing a GPC3 antibody. The folate reductase defect type CHO cells are lack of endogenous dihydrofolate reductase, when an expression vector containing a dihydrofolate reductase gene and a target gene is co-transfected, the dihydrofolate reductase gene and the target gene are generally integrated at the same site of a host chromosome, and then in the presence of a competitive inhibitor-methotrexate of dihydrofolate reductase, the increasing of the copy number of the dhfr gene is driven to achieve the purpose of increasing the expression quantity of the target gene, so that a stable cell line for stably and efficiently expressing GPC3 is constructed, and the technical blank is filled.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a recombinant CHO cell strain stably expressing GPC3 and an application thereof. Background technique [0002] Hepatocellular carcinoma (HCC) is the fifth most common cancer with a high incidence in the world. About 110,000 people die from liver cancer every year in my country, accounting for 45% of the world's liver cancer deaths. At present, surgical resection of the tumor is still the best and fastest method for the treatment of HCC. However, most patients have lost the best opportunity for treatment when they go to the doctor, and only 10-20% of them can be surgically resected early. Even if they are surgically resected, the recurrence rate is still high. Therefore, early detection, early diagnosis, and early treatment of HCC are the key to improving the survival rate of patients, as well as the key to prognosis and treatment. [0003] Glypican 3 (glypican-3, GPC3), its gene is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/10C12N15/12C12N15/85C07K16/30C12R1/91
CPCC07K14/4748C07K16/303C12N15/85C12N2800/22
Inventor 李伟赵叔杰
Owner 南京拂晓生物科技有限公司
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