41results about How to "Save experiment cost" patented technology

The invention relates to a rock sample detection and data acquisition system. The system can realize elastic parameter test of a rock sample in the state of gas adsorption; in the system, a three-shaft pressure cabin is connected with a gas supply and metering control device for absorbing samples in the cabin and accurately metering absorption quantity, moreover, an ultrasonic transducer is installed on a cap of the (high) pressure cabin and integrated with an ultrasonic wave transmitting / receiving device so that the test of elastic parameters of methane absorption on coal can be realized truly. The system can automatically and continuously test in the processes of gas absorption and gas enrichment, thereby ensuring a consistent test environment, acquiring an absorption quantity and elastic parameter relation curve, improving test accuracy and efficiency and saving test time and cost. The rock sample detection and data acquisition system integrates methane enrichment mechanism simulation and rock physical property test, can be applied to seismic prospecting method research on coalbed methane and shale gas, mine advanced detection, coal mine safety research and the like.

The invention discloses a method for simultaneously compensating a scale factor and a zero offset of an optical fiber gyroscope by a one-time temperature experiment. The method comprises the following steps: (1) adjusting the temperature of an incubator, rotating the gyroscope clockwise and anticlockwise when the temperature reaches respective set temperature values, and acquiring temperature data acquired by a temperature sensor in the gyroscope in real time and data which correspond to the temperature and are output by the gyroscope in the whole process of switching on and switching off the incubator; (2) processing the temperature data and the data output by the gyroscope according to a simultaneous scale factor and zero offset compensation algorithm, and obtaining a temperature compensation coefficient matrix; (3) compensating the output of the gyroscope according to the temperature compensation coefficient matrix. According to the compensation method provided by the invention, the one-time temperature experiment is designed to simultaneously compensate the scale factor and the zero offset of the optical fiber gyroscope; a relation between a digital value output by the gyroscope and an input angle speed is directly expressed, so that the compensation coefficient matrix is obtained; therefore, the inaccuracy, which is caused by a zero offset error and the like, of compensation of the scale factor is avoided; furthermore, the temperature error of the gyroscope can be effectively compensated, and the method can save the experiment cost and is higher in practical significance.

The invention provides a multifunctional water-entering slamming experiment casting device. A pair of No.1 T-shaped brackets are installed on the upper end of a device fixing rack, an angle adjustment rotating shaft is installed between the two No.1 T-shaped brackets, an angle adjusting plate is installed on the side surface of the device fixing rack, an angle fixing groove is formed in the angle adjusting plate, the upper part of a linear motion bracket is fixedly connected with the end part of the angle adjustment rotating shaft, the lower part of the linear motion bracket is installed in the angle fixing groove in an adjustable manner via a screw, the top end of the linear motion bracket is provided with a motor in an inverted manner, an output shaft of the motor is connected with a lead screw, a linear motion pedestal is installed on the lead screw, a pair of No.2 T-shaped brackets are installed on the linear motion pedestal, a motion supporting rod is installed between the two No.2 T-shaped brackets, and the lower end of the motion supporting rod is provided with a structure to be casted. The multifunctional water-entering slamming experiment casting device is simple and convenient in structure, is easy to install and detach, has the advantage of functional diversification, can realize the multi-type water-entering casting function, satisfies precision of multifunctional water-entering experiments, and can save experimental expense and cost.

The invention relates to a composite material structure based fluid-solid coupling value prediction method, and belongs to the technical field of impeller mechanical simulation. The method comprises the steps that a composite material hydrofoil structure model is built, mesh generation is conducted, a three-dimensional basin is built, mesh generation is conducted on the three-dimensional basin, and a computational fluid dynamics model is built; initial steady flow field numerical calculation and unsteady flow-solid coupling numerical calculation are conducted, calculation results are subjectedto postprocessing, and the dynamic change process of the flow field structure and composite material hydrofoil deformation over time is obtained. Accordingly, influences of the fluid viscosity and the three-dimensional shape of the structure are fully considered, and the reliability of the numerical calculation result is enhanced; in addition, high-precision numerical prediction on the compositematerial hydrofoil fluid-solid coupling phenomenon can be achieved.

The invention discloses a physical simulation experiment system and method of deep-tunnel high-resistance extensible anchor pole support. The physical simulation experiment system comprises a simulation experiment table, a pressure loading apparatus, high-resistance extensible anchor poles, a tunnel model, a surrounding rock displacement sensor, an anchor pole dynamometer, an anchor pole dynamometer, a pole tail sleeve displacement sensor, a digital display screen, a data emission apparatus, a data receiving apparatus, a display and a data processing apparatus. The pressure loading apparatus is arranged at equal intervals between the outer side of the tunnel model inside the simulation experiment table, the multiple high-resistance extensible anchor poles are arranged between the anchorage zone rock and the hole of a tunnel, output of the surrounding rock displacement sensor, the anchor pole dynamometer and the pole tail sleeve displacement sensor is sent to the data processing apparatus for processing and is displayed by the display. The system provided by the invention is reasonable in structural design and simple to operate, enables people to visually observe deformation and support working states of the anchor pole and realizes simulation of the support mechanics characteristics and the support process of the anchor poles.

The embodiment of the invention provides a calculation method and system for numerical simulation of a rockfill concrete pouring process. The calculation method comprises the following steps: according to a finite element / discrete element coupling analysis method FEM / DEM, calculating and analyzing a stacking process and a final stacking form of a three-dimensional model of an initial state of a rockfill block; after calculating and analyzing the packing process and final packing state of the initial state three-dimensional model of rockfill, calculating and analyzing the pouring process and final filling state of self-compacting concrete in the final packing state of rockfill based on the SPH method. The method and the system provided by an embodiment of the present invention can accurately simulate the pouring process of self-compacting concrete, and not only can the flow state of self-compacting concrete in rockfill pores be observed directly, but also the final compaction state ofrockfill concrete can be known, which saves a lot of manpower cost, experiment cost and economic cost, and avoids the difficult problem that the experiment method is difficult to observe on-line.

The invention relates to an experimental device used for testing minimum bending radius of an ocean flexible pipe cable. The experimental device is characterized by comprising two panels which are the same in shape and size and matched bolts, wherein the lower part of each panel is provided with horizontal bolt holes arranged into a horizontal line at intervals, the panels above the horizontal bolt holes are also provided with a plurality of groups of arc-shaped bolt holes, and each group of the arc-shaped bolt holes and the horizontal bolt holes can form configuration lines with different bending radiuses. The experimental device provided by the invention is simple to operate and convenient to manufacture and has the advantages of saving experimental time and reducing cost.

The invention provides a method and a device for measuring the continuous cooling phase transformation points of aluminum alloy. The method comprises the following steps of automatically collecting the data of resistance, temperature and time in real time by utilizing a computer, drawing a voltage-temperature curve or a resistance-temperature curve of an aluminum alloy sample according to the measured data, and then obtaining the cooling phase transformation points according to the drawn curve. The device comprises a heating insulation and cooling system, a dynamic voltage measuring system, a temperature measuring system, a signal amplifying and converting system and a data collecting and processing system which are sequentially connected. The device can be used for obtaining the curve of voltage changing along with the temperature in the continuous cooling process of a supersaturated solid solution, thereby judging the phase transformation points of the alloy. The invention has the advantages of wide cooling test range, high measuring precision, high speed and simple operation, and can carry out heating, insulation, cooling, and the voltage and temperature real-time measurement and collection on aluminum alloy materials.

The invention discloses an ultra-low temperature extraction device and an analysis method of smoke aroma components by using the ultra-low temperature extraction device combining with a full two-dimensional gas-phase time-of-flight mass spectrometer. Compared with a conventional method, the analysis method is low in price, easy to operate, free from high temperature and high pressure treatment in separation and enrichment processes thus guaranteeing the characteristics of original aroma components in smoke, being capable of being used for collecting and enriching aroma components in particulate matters and gaseous matters of the smoke simultaneously, and being capable of being used for detection by utilizing the full two-dimensional gas-phase time-of-flight mass spectrometer simultaneously, so that the device and the method have the advantages of good degree of separation, high sensitivity and accuracy, and high analysis speed.

The invention discloses a dynamic pressure regulating gas absorption and desorption experiment device based on a high-pressure volumetric method. The dynamic pressure regulating gas absorption and desorption experiment device comprises a dynamic pressure regulating inflation system, a vacuum degassing system, a gas absorption and desorption system, a constant-temperature system, a metering systemand a data processing system, wherein the dynamic pressure regulating inflation system is provided with a high-pressure gas cylinder, a pressure regulating valve, a high-pressure valve, a pressure gauge, a dynamic pressure regulating inflation tank and a three-way connector; the vacuum degassing system is provided with a vacuum pump, a motor, a vacuum diaphragm valve, a vacuum gauge tube, a vacuumgauge, a vacuum tank path, a high-pressure valve and a three-way connector; the gas absorption and desorption system is provided with an absorption and desorption tank, a pressure gauge and a high-pressure valve; the constant-temperature system is provided with a super constant-temperature water bath tank; the metering system is provided with a flow meter, a leveling bottle, a measuring cylinder,a glass piston, a sampling bag and a lifter; the data processing system is provided with a pressure sensor, a data collector, a computer and a printer. The dynamic pressure regulating gas absorptionand desorption experiment device disclosed by the invention has the advantages of convenience in operation, high experiment accuracy, accurate results and ability in achieving gas absorption and desorption experiments.

The invention relates to a method for correcting a predictor formula for conventional tar and nicotine in cigarettes, and belongs to the technical field of tobacco. The method mainly comprises the following steps: by lofting research, the reduction rate of the ventilation rate of a filter tip by the tar and nicotine is worked out, the reduction rate is corrected in the predictor formula for cigarette tar and nicotine in cigarettes, so that the corrected formula can be used for cured tobacco type cigarettes and cigarettes of any tobacco blending formula. After being corrected, the predictor formula can improve the prediction precision of the tar and nicotine in cigarettes greatly; and when used to design products, the corrected formula can save design cost and test cost of the products, thereby greatly improving the scientificity of cigarette product development.

The invention discloses a diameter adjustable liquid bridge generator and belongs to the technical field of crystalline material preparation. The diameter adjustable liquid bridge generator comprises a support frame, wherein an upper liquid bridge support column mounting hole is formed in the upper part of the support frame and a lower liquid bridge support column mounting hole is formed in the lower part of the support frame; an upper liquid bridge support column is vertically arranged inside the upper liquid bridge support column mounting hole, a lower liquid bridge support column is vertically arranged inside the lower liquid bridge support column mounting hole, the upper liquid bridge support column corresponds to the lower liquid bridge support column, and a fastening bolt of the upper liquid bridge support column is arranged on the support frame located on the side of the upper liquid bridge support column mounting hole; an upper liquid bridge support column cap is fixedly arranged on the upper end of an upper liquid bridge support column king rod, and an upper liquid bridge support column upper case is arranged at lower end of the upper liquid bridge support column king rod through a screw; a lower liquid bridge support column lower bar is fixedly connected with the lower end of a lower liquid bridge support column king rod through a lower liquid bridge support column snap ring; and the upper end of the lower liquid bridge support column lower bar is connected with the lower liquid bridge support column lower case through the screw.

The invention relates to an on-line monitoring method of a tightly sealed labyrinth gap and a leakage flow, which can carry out online real-time monitoring on the labyrinth tightly sealed gap and thelabyrinth leakage flow, and can adjust engine parameters in real time according to the monitored data, so as to improve the safety and reliability of the operation of an aviation engine and save aviation fuel consumption. The safety and reliability of the on-line monitoring method of the tightly sealed labyrinth gap and the leakage flow are mainly reflected in being able to solve the problems suchas the rotation-static friction caused by changes in gap during engine operation, the changes of the axial force caused by pressure changes, the invasion of gas in the radial direction, the oil leakage, and overheating of components due to reduced cool gas flow, which greatly improves the operation efficiency of the engine.

The invention provides an underground space fire hazard simulation experiment device, and relates to the technical field of fire fighting safety. The device comprises an experiment tunnel, a data acquisition system, a fire source and an automatic oil supplementing device, wherein the experiment tunnel comprises a rectangular tunnel, a movable baffle plate and an arc-shaped cover; an integral framework of the rectangular tunnel is an angle steel welding component; fireproof glass is arranged on the wall surface of the tunnel; the arc-shaped cover is a steel plate component; the movable baffle plate with uniformly distributed smoke discharging openings is arranged at the upper part of the rectangular tunnel; the movable baffle plate and the rectangular tunnel are fixedly connected through screw holes; the bottom of the rectangular tunnel is provided with a combustion basin; the bottom of the combustion basin is connected with the automatic oil supplementing device through a pipeline; thesmoke discharging openings are separately arranged; the four smoke discharging units form one unit and are connected with a smoke collecting cover through a smoke discharge pipeline; a centrifugal fan is arranged at an outlet of the smoke discharging pipeline; a jet flow fan is arranged at the upper part of the rectangular tunnel. The underground space fire hazard simulation experiment device ismainly used for underground space fire hazard simulation experiments.

The invention discloses a kit for rapidly detecting the SNP site of a Y chromosome and a method, and the method is applied to the rapid identification of Y chromosome haplotype pedigrees of male individuals in forensic science. The rapid detection kit for the identification of the characteristic sites of the Y chromosome haplotype pedigrees of East Asian males includes primers corresponding to theSNP characteristic sites used for amplification detection of the Y chromosomes and probes of two mutation type sequence characteristics representing the SNP sites. The using method includes adding asample into plates having 96 or 384 pores, and performing an amplification reaction in a PCR instrument after a PCR reaction system is well mixed; putting the plates into the quantitative PCR instrument having an SDS scanning function to read a fluorescence signal after the amplification is finished; and automatically analyzing the Y-SNP haplotype of the sample by the instrument according to the fluorescence signal, and outputting a result in a spreadsheet. Through the kit, the detection can be completed by one step amplification, purification and template preparation are not needed, and the detection process of the Y-SNP can be simplified.

The invention relates to a solid-phase extraction device. The solid-phase extraction device comprises a sampling pump, a pressure meter, a flow meter and a vertically arranged solid-phase extraction unit which are connected with one another in series, wherein the solid-phase extraction unit comprises a lower external adapter, an upper external adapter, a connector and two filtration tanks; the top of the lower external adapter is fixedly connected with the bottom of a first filtration tank; the top of the first filtration tank is detachably connected with the bottom of the connector; the top of the connector is detachably connected with the bottom of a second filtration tank; the top of the second filtration tank is fixedly connected with the bottom of the upper external adapter; the bottom of the lower external adapter and the top of the upper external adapter penetrate through a flow guide pipe; the first filtration tank, the second filtration tank and the connector are internally connected with one another; annular limiting slots are formed in the periphery of the inner wall of the connector; a non-return pressing sheet, a solid-phase extraction film and a support screen plate are arranged in sequence from top to bottom of each limiting slot. The solid-phase extraction device is easy to operate and convenient to transport, and is capable of preprocessing water samples on sampling sites.

An anchor rod static and dynamic tension shear comprehensive experiment device comprises a base, a static tension execution mechanism, a static shear execution mechanism, a dynamic tension shear execution mechanism and a shear box mechanism. The static stretching executing mechanism, the static shearing executing mechanism and the dynamic stretching and shearing executing mechanism are arranged on the base, the shearing box mechanism is arranged on the static stretching executing mechanism, and the static stretching executing mechanism, the static shearing executing mechanism, the dynamic stretching and shearing executing mechanism and the shearing box mechanism are distributed on the same straight line. According to the anchor rod static and dynamic tension-shear comprehensive experiment device, an anchor rod static tensile experiment, an anchor rod static shear experiment, an anchor rod dynamic tensile experiment, an anchor rod dynamic shear experiment, an anchor rod static shear experiment in a tensile stress state and an anchor rod dynamic shear experiment in a tensile stress state can be completed in the same equipment, and multiple purposes of one machine are achieved; the test of the comprehensive performance index of the anchor rod can be met only through one device, and the device purchase cost and the experiment cost are effectively saved.

The invention relates to a method of testing the elasticity of a PTT / PET (polytrimethylene terephthalate / polyethylene glycol terephthalate) self-crimping filament by using hank knotting thereof. The method is characterized by comprising the following steps: step 1, making self-crimping filament hank knotting; step2, performing dry heat treatment to the hank knotting; step3, carrying out hank knotting tensile testing; step 4, calculating the elastic elongation ratio. The method has the following beneficial effects: (1) the elastic property of the self-crimping filament can be accurately predicted in fabric at a fiber stage, thus the experimental cost and time are greatly reduced in contrast with a current method of testing the elasticity after trial-producing the fabric; (2) the operation is simple, and the test method is conveniently applied to industrial production; (3) the representativeness of the elastic elongation ratio of the hank knotting is greatly improved in contrast to that of a monofilament, thus the experimental times are reduced, and the experimental precision is improved.

The invention discloses a xenopus laevis oocyte expression carrier with a yellow or red fluorescence protein label and application. pGH19 plasmid is modified through a molecular biology means, and isspecifically developed to be a carrier pGH19-EYFP or pGH19-mRFP which is suitable for expression of the xenopus laevis oocyte and contains the enhanced yellow or monomer red fluorescence protein label. The carrier can normally express the target gene, and can detect the distribution situation and the expression mode of the target protein with the yellow or red fluorescence label through a fluorescence microscope or a confocal microscope, and thus a more visual and simpler detection method can be provided for the protein situation of xenopus laevis oocyte expression. The spatiotemporal dynamicsof the protein expression can be observed in real time. The expression situation of the target protein can be detected only by using an universal antibody as for the following extraction, purification, identification and functional analysis of the target protein, the experimental process is greatly simplified, and the experimental time is saved and economic cost is reduced.

The invention discloses an experimental device for simulating the impact of the upstream dam break on a downstream dam. The experimental device comprises a water tank, an upstream arch dam model baffle and a downstream arch dam model baffle. The upstream arch dam model baffle and the downstream arch dam model baffle are arranged in the water tank. At least two levels of storage capacities are set in the water tank on the upstream position of the upstream arch dam model baffle. The adjacent two levels of storage capacities are partitioned by partitions which can adjust the capacity of the storage capacities. A water injection device for injecting water into the water tank is arranged above the water tank. A monitoring instrument for obtaining the flood routing characteristic of the upstream arch dam model baffle and the characteristics of the impact and destroy of the flood on the downstream position is arranged on the downstream arch dam mold baffle. The experimental device is simple in structure, the experimental scheme is convenient to adjust and optimize, the operability and practicability are high, and the experimental device can be widely applied to dam break and cascade dam break model experiments.

The invention provides an SNP molecular marker related to wheat stem rot resistance and an application of the SNP molecular marker. Whole genome association analysis is carried out on a natural population composed of 358 Chinese wheat germplasm resources, and five SNP loci closely associated with stem rot resistance are found on a hexaploid wheat 5D chromosome long arm for the first time. KASP primers capable of being used for large-scale material screening are further developed for the SNP loci, and the close association degree of the developed molecular marker and disease resistance is verified in F2 and F2: 3 populations constructed by using an anti-infection material as a parent. The five SNP molecular markers provide an effective means for screening of wheat anti-FCR germplasm resources.

The invention relates to an electric furnace applicable to a thermal state experiment of blast-furnace cooling staves with different sizes. The technical scheme is as follows: the electric furnace applicable to the thermal state experiment of the blast-furnace cooling staves with different sizes consists of a furnace bottom (1), a furnace wall (2), a group of heating pipes (3), a cooling stave (4), an embedded brick (5) and a bearing frame (6), wherein the furnace wall (2) is a combined furnace wall with adjustable lengths and widths; the group of heating pipes (3) are installed in through holes of the furnace wall (2); the cooling stave (4) is installed on the bearing frame (6) by bolts (7) and forms a hearth together with the furnace wall (2). The electric furnace is applicable to cooling staves with different sizes, and has the characteristics of accurately controlling experimental temperature, being convenient to operate, safe and environmentally-friendly, and the like by adoption of electrical heating.

The invention provides a method for quantitatively determining viability of barley seeds, belongs to the technical field of evaluation of the viability of the barley seeds, and can evaluate germination potential of the barley seeds rapidly and stably and provide reference for seed purchase and production. According to the method, TTC (triphenyl tetrazolium chloride) is used for dyeing dissected barley seeds, the dyed barley seeds are crushed with a freeze-drying and ball-milling crushing combined technique, a 10% trichloroacetic acid methanol solution is adopted as extract liquor to perform extraction on the crushed barley seeds, TTF (1,3,5-triphenylformazan) is obtained, and content of TTF is analyzed quantitatively according to a TTF standard curve with absorption photometry to predict the viability of the barley seeds. With the adoption of the freeze-drying and ball-milling crushing combined technique, loss of a sample in an operation process is reduced, and an experimental result is more precise and more reliable. By separating and dissecting the barley seeds, interference of useless substances in follow-up experiments is reduced, reaction volume is reduced, and experiment costand workload are saved.

The invention belongs to the technical field of soil microbial community diversity analysis and discloses optimal conditions and reagent dosage in a PCR-DGGE (Polymerase Chain Reaction Denaturing Gradient Gel Electrophoresis) method used for analyzing microbial community diversity of soil polluted by petroleum. The analysis method comprises the following steps of: 1, extracting enough DNA from 0.4g of soil samples; 2, carrying out PCR (Polymerase Chain Reaction) on 1 microliter of purified DNA; 3, performing a DGGE (denaturing gradient gel electrophoresis) experiment on 3-5 microliters of PCR products; 4, adding 9 microliter of TEMED (tetramethylethylenediamine) and 40 microliter of 10% APS (ammonium sulfate) into 18ml of modified acrylamide glue solution for preparing gel; 5, filling the gel, covering the top with a preservative film, standing still overnight to finally obtain excellent modified gel; 6, performing electrophoresis for 5 hours at the voltage of 120V and the electrophoresis temperature of 60 DEG C to obtain a good DGGE map; and 7, performing silver staining, wherein a stop solution is not used necessarily. The method is mainly used in the molecular ecology researches in the bioremediation process of soil polluted by petroleum, and also can provide references for molecular ecology researches of other environmental samples.

The invention discloses a primary isolated culture and induced differentiation method for precursor fat cells in adult yak muscles. The method comprises the steps of disinfection treatment and segmentation, digestion, screening and filtration, centrifugation, purification and culture. According to the cell culture for the precursor fat cells in the adult yak muscles, provided by the invention, manpower and material resources can be saved, and the limitation of high pollution rate caused by using newborn calf fat tissues and primary cells in a traditional culture method is broken through, so that the source approach of samples is wider, and a large number of cells can be obtained at the same time; and the culture method is a convenient and efficient precursor fat cell culture method, provides a molecular basis for subsequent in-depth research on gene and protein expression, signal channels and the like in proliferation and differentiation process of intramuscular precursor fat cells, provides a new thought and a new method for research on fat deposition, fat metabolism, fat mobilization and the like of different parts, and has an important significance in improving the meat quality of yaks.

The invention discloses a volume and weight measuring device and method for vegetables and fruits in irregular shapes. The device comprises a base, a water storage tank arranged on the base, a weighing sensor arranged at the upper end of the water storage tank and a sample box arranged at the upper end of the weighing sensor, and a sample box water inlet pipe is arranged at the top end of the sample box; a sample box downcomer on the side surface of the lower end of the sample box is provided with a sample box downcomer manual valve; the top of the water storage tank is provided with a first funnel-shaped water receiving port which is opposite to the outlet of the sample box downcomer; a water pump is arranged at the bottom of the water storage tank; the outlet of a water feeding pipe connected with the water pump is opposite to a second funnel-shaped water receiving opening in the top end of the water inlet pipe of the sample box, a funnel-shaped water inlet is formed in the top end of the water storage tank, a liquid level meter is arranged on the side face of the water storage tank, an electric control box with a rechargeable battery is arranged on the side face of the water storage tank, and a sample box liquid level sensor is arranged in the sample box. The volume and weight measuring device and method are suitable for field edges, are convenient to move, and can accurately measure the volume and the weight of vegetables and fruits with irregular shapes.

The invention relates to a transformer oil quality comprehensive evaluation method based on multi-frequency ultrasound, and belongs to the field of transformer oil aging degree detection. The method comprises the following steps: S1, carrying out multi-frequency ultrasonic testing on transformer oil samples with different operation years, and independently detecting electrical insulation parameters of each oil sample by adopting a traditional parameter detection method; s2, calculating and scoring the electrical insulation parameters by utilizing an improved analytic hierarchy process and an expert weight matrix, and then giving the state grade of each oil sample according to the score; s3, using multi-frequency ultrasonic test data as input, taking the state grade of each oil sample as output, and using an SVM for establishing a transformer oil quality comprehensive evaluation model; and S4, obtaining an acoustic frequency spectrum of the transformer oil sample to be detected, and judging the quality of the transformer oil to be detected by utilizing the transformer oil quality comprehensive judgment model. According to the invention, the experiment time and the experiment cost are greatly saved, and the method has important significance for ensuring the safe and reliable operation of the oil-immersed power transformer.

The invention relates to the field of vehicle transmission systems, and particularly discloses a two-gear mechanical automatic transmission for an electric vehicle. The two-gear mechanical automatic transmission comprises a shell, an input shaft and an output shaft, wherein the shell internally comprises a double-row two-stage planetary gear set, a first brake and a second brake, the double-row two-stage planetary gear set includes a front-row planetary gear set and a rear-row planetary gear set, the rear-row planetary gear set and the front-row planetary gear set are both connected to a planetary carrier, the rear-row planetary gear set and the front-row planetary gear set are meshed, and the rear-row planetary gear set is meshed with a rear-row sun gear; the first brake is used for braking the planet carrier, and the input shaft drives the rear-row sun gear to rotate; and the second brake is used for braking the front-row planetary gear set, and the front-row planetary gear set drives the output shaft to rotate. According to the scheme, the first brake, the second brake and the double-row two-stage planetary gear set are matched, and the problem that in the proper art, during climbing or low-speed starting of the electric vehicle, the power is insufficient, the transmission is poor in gear shifting performance, and the transmission is poor in scalability are solved.

The invention relates to a method of lettuce isolated-culture high-frequency regeneration. The method includes the steps that cotyledons and hypocotyls of aseptic lettuce seedlings serve as explants to be subjected to isolated culture and are induced to bud on a budding culture medium, and then, buds are transplanted to a rooting culture medium to form a mass of seedlings. Main components of the budding culture medium include 0.2 mg / L of an MS basic medium, 0.5 mg / L of NAA, 30 g / L of 6-BA and 8 g / L of agar; main components of the rooting culture medium include 0.1 mg / L of an MS basic medium, 30 mg / L of IAA, 30 g / L of sucrose and 8 g / L of agar. According to the method of lettuce isolated-culture high-frequency regeneration, the specific hormone-proportioned media are adopted, the culture period is shortened by 7-10 days compared with current regeneration systems, the differentiation frequency of the explants can achieve 100%, it can be ensured that the seedling regeneration rate reaches95% or above, and experiment and labor costs are saved.

The invention discloses a synthesis method of an alpha-ketoamide compound. The preparation method comprises the following steps of: taking an isocyanate compound as shown in a formula I and a benzoylformic acid compound as shown in a formula II as raw materials, and carrying out heating reaction to obtain the alpha-ketoamide compound as shown in a formula III, wherein the reaction formula is described in the specification, and in the formula, R1 and R2 are independently selected from alkyl, substituted alkyl, aryl, substituted aryl or aromatic heteroradical. The synthesis method has the advantages that the synthesis steps are simple, the operation is safe, the compatibility of the synthesis method with functional groups is good,, the alpha-ketoamide compound with very high additional value is obtained, the yield can reach 92%, the purity is 99%, the experiment cost or the production cost is greatly reduced, industrial synthesis is easy, and another new synthesis thought is provided for constructing more alpha-ketoamide drugs.