The invention discloses an artificial culture method of Zn-enriched cordyceps militaris mycelia and a culture medium thereof; according to weight percentage, the formulation components of the culture medium comprise: 1.0 to 3.0 percent of glucose, 0.5 to 1.0 percent of peptone, 0.1 to 1.0 percent of yeast extract, 1.0 to 2.0 percent of agar powder and the rest of water, and the pH value of the culture medium is 5.0 to 7.5. The artificial culture method comprises the steps of test tube slant surface strain culture (activation), test tube liquid strain culture, triangular flask liquid seed culture, seeding tank culture, fermenter culture, plate-frame filtration, crashing, drying in vacuum, crashing and leading the mycelia into powder. The operation is simple, the culture period is short, the yield of mycelia is high, no pollution and waste are produced, and the method has the function of supplementing zinc, thus being capable of carrying out industrial production.