Zinc-rich yeast refined extract and preparation method thereof
A zinc-enriched yeast and yeast extract technology, applied in pharmaceutical formulations, cosmetic preparations, cosmetic preparations, etc., can solve problems such as adverse skin irritation, achieve safe absorption and utilization, and eliminate the effects of irritation
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[0019] According to a typical implementation of the present invention, when the dry matter mass percentage is 5%, the absorbance of the yeast extract at a wavelength of 410nm is lower than 0.15, which ensures that the color of the yeast extract is close to colorless , Odorless, suitable for coloring and seasoning of cosmetics, has the advantages of strong applicability and wide application fields.
[0020] The raw material of the present invention is a yeast extract product prepared from Saccharomyces cerevisiae or other species of yeast, which can be purchased from the market or obtained by extracting and separating Saccharomyces cerevisiae or other species of yeast.
[0021] The present invention also provides a method for preparing the above-mentioned yeast extract. According to a typical embodiment of the present invention, the preparation method includes the following steps: 1) preparing the yeast extract, and adjusting the zinc content to 5000-20000mg / kg Zinc-rich yeast ...
Embodiment 1
[0028] 1) Prepare yeast extract. Zinc-enriched yeast (zinc content 5000mg / kg) 150kg, add 850kg of purified water, prepare a suspension with a mass percent content of 15%, heat to 55°C, keep warm for 8h, adjust the pH to 6.0, add 1500g of papain, and react for 20 After 1 hour, it was heated to 95° C., incubated for 1 hour, and the supernatant was collected by centrifugation (5000 g centrifugal force, 10 minutes).
[0029] 2) Membrane separation. The above-mentioned yeast extract is formulated into an aqueous solution with a mass percentage of dry matter of 2%, and then the ultrafiltration membrane with a molecular weight cut-off of 20-500KD is used for separation, and a ceramic membrane with a molecular weight cut-off of 500KD is used for membrane separation. Serum.
[0030] 3) High temperature treatment. The supernatant was concentrated in vacuo until the mass percentage of dry matter was 10.2%, and was incubated at 100° C. for 1 hour.
[0031] 4) Decolorization. The supe...
Embodiment 2
[0034] 1) Prepare yeast extract. Add 50kg of zinc-rich yeast (zinc content 20000mg / kg), add 950kg of purified water, prepare a suspension with a mass percentage of 5%, heat to 65°C, keep it warm for 2h, adjust the pH to 5.0, add 500g of papain, and react for 5 After 1 hour, heat to 100° C., keep warm for 2 hours, and collect the supernatant by centrifugation (5000 g centrifugal force, 10 minutes).
[0035] 2) Membrane separation. The yeast extract and purified water are prepared to prepare an aqueous solution with a mass percentage of dry matter of 20%, and a ceramic membrane with a molecular weight cut-off of 100KD is used for membrane separation, and the permeated supernatant is collected to obtain the supernatant. 3) High temperature treatment. The supernatant was concentrated in vacuo until the mass percentage of dry matter was 8.2%, and treated at 120° C. for 0.5 h.
[0036] 4) Decolorization. The supernatant was adjusted to pH 5.0 with hydrochloric acid, and the temp...
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