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Selenium-enriched arachidonic acid oil product, selenium-enriched mortierella alpina and preparation method

A technology of arachidonic acid and Mortierella alpine, applied in biochemical equipment and methods, microorganism-based methods, microorganisms, etc., can solve the problems of insufficient resource utilization, weak selenium conversion ability, and a lot of time and labor. , to achieve the effect of eliminating toxic side effects and gastrointestinal irritation, improving selenium conversion ability, and safe absorption and utilization

Active Publication Date: 2019-04-23
CABIO BIOTECH WUHAN CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Mortierella alpina is currently a common bacterial species producing arachidonic acid oil, but Mortierella alpina strains only produce microbial oils, with single products, single nutrition, and insufficient resource utilization; common Mortierella alpina has a relatively low Se conversion capacity Weak, poor tolerance to selenium, selenium-enriched Mortierella alpina needs to be obtained through screening and domestication, but screening and domestication requires a lot of time and manpower

Method used

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  • Selenium-enriched arachidonic acid oil product, selenium-enriched mortierella alpina and preparation method
  • Selenium-enriched arachidonic acid oil product, selenium-enriched mortierella alpina and preparation method
  • Selenium-enriched arachidonic acid oil product, selenium-enriched mortierella alpina and preparation method

Examples

Experimental program
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Effect test

Embodiment 1

[0063] The preparation method of the selenium-enriched Mortierella alpina provided by the present embodiment is as follows:

[0064] Strain: Mortierella alpine Y16 (Mortierella alpine Y16) has a preservation number of CCTCCNO.M2015421.

[0065] 1 Preparation of spore suspension:

[0066] Inoculate the Mortierella alpina strain on a potato dextrose agar (PDA) medium plate, culture it at 28°C for 6 days until the spores form and mature, remove the hyphae and spores on the medium, and prepare spores with sterile water Suspension.

[0067] 2 seed cultures:

[0068] The spore suspension was inoculated into shake flasks for cultivation, the inoculum size was 10 (volume ratio), the cultivation temperature was 28° C., the cultivation time was 48 hours, and the shaking speed of the shaker was 220 rpm.

[0069] The seed medium in the shake flask includes: glucose 20g / l; yeast powder 15g / l; pH 5.5-8.5.

[0070] 3 fermentation:

[0071] Seed shake flask fermentation: when the bacteri...

Embodiment 2-19

[0103] The Mortierella alpina fermentation method and detection method of Examples 2-19 are basically the same as those in Example 1. The main difference of each example lies in the addition of selenium salt. The differences are shown in Table 1.

[0104] Table 1

[0105]

[0106]

[0107] Note: "-" in the table means not added.

[0108] Wherein the bacterial strain that embodiment 16,17 and comparative example 2 adopt is that this bacterial strain was preserved in China Type Culture Collection Center (CCTCC) on September 13, 2013 by Carbio Bioengineering (Wuhan) Co., Ltd., preservation address Yes, China, Wuhan, Wuhan University, deposit number CCTCCNO:M2013419.

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Abstract

The invention discloses a selenium-enriched arachidonic acid oil product, selenium-enriched mortierella alpina and a preparation method. The preparation method comprises the step of fermentation, wherein selenium salt is added into a fermentation culture medium inoculated with mortierella alpine. The preparation method increases the content of selenium in the mortierella alpine through the optimization and improvement of the addition time and addition process of the selenium salt, and prepares the mortierella alpina rich in selenium and arachidonic acid oil.

Description

technical field [0001] The invention relates to the technical field of selenium-enriched fermentation, in particular to a selenium-enriched arachidonic acid oil product, selenium-enriched Mortierella alpina and a preparation method. Background technique [0002] The benefits of selenium to the human body: Selenium (Se) is determined by the World Health Organization and the Chinese Medical Association as the third largest micronutrient and health element that must be supplemented after iodine and zinc in the 21st century. It has anti-oxidation, anti-cancer, detoxification, and Improve immunity and so on. [0003] At present, all kinds of selenium supplement products that are artificially added are mainly divided into inorganic selenium and organic selenium. Inorganic selenium is mainly sodium selenite, which is mostly used in feed abroad, has a lot of toxins, and is on the verge of technology elimination. Compared with inorganic selenium such as sodium selenite, organic sele...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C12P1/00C12R1/645
CPCC12P1/00C12P7/6436C12N1/145C12R2001/645
Inventor 汪志明陆姝欢余超熊淑婷李翔宇
Owner CABIO BIOTECH WUHAN CO LTD
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