36results about How to "Reduce lab consumables" patented technology

The invention discloses an erosion ditch monitoring method based on close-range photogrammetry. The erosion ditch monitoring method comprises the steps that a photographing target is arranged around an erosion ditch, and a total station or an RTK-GPS is adopted for measuring a target center point, and position information is obtained; a non-measuring common digital camera is adopted to rotate around the erosion ditch clockwise (anticlockwise) and manual photographing is carried out; the photo is guided into Agisoft Photoscan software, and the sparse point cloud, dense point cloud and grids are established through camera calibration, photo splicing, target detection operation and the like, and the coordinates of the target point are guided to obtain a digital elevation model and a digital positive projection image of the erosion ditch; and the digital elevation model of the high-precision erosion ditch is guided into ArcGIS 10.2 software, and form parameters and erosion amount information of the erosion ditch are extracted. The method has the advantages that the dependence and the requirement degree of the instrument are low, the cost is low, the experimental consumable is small, the high-quality digital elevation model data can be quickly obtained, and the information of the erosion ditch can be conveniently and accurately extracted.

The invention discloses a pair of RT-PCR ( reverse transcription-polymerase chain reaction) primers and a kit for detecting high-pathopoiesia pig blue ear disease and classic pig blue ear disease. The upstream primer nucleotide sequence of the primers is GGCGACAATGTCCCTAAC, the downstream primer nucleotide sequence of the primers is GATGGCTTGAGCTGAGTAT, and the kit contains the primers. The primers and the kit for detecting high-pathopoiesia porcine reproductive andrespiratory syndrome and classic porcine reproductive andrespiratory syndrome have the advantages of high sensibility, strong specificity, rapidness and convenience, low detecting cost and the like.

A preparing method of a human DCCIK immunocompetent cell is disclosed. The method includes steps of: (1) separating a mononuclear cell from human peripheral blood; (2) inoculating the mononuclear cell to a culture medium suitable for lymphocyte culturing, adding IFN[gamma], IL-2, an anti-CD3 monoclonal antibody, GM-CSF and IL-4, and culturing for 3-5 days; (3) adding TNF[alpha], and continuously culturing for 1-2 days; and (4) adding IL-2, and continuously culturing for 7-10 days to obtain the DCCIK cell. A composition of various cytokines and stimulating factors is added directly into the mononuclear cell so that the DC cell and lymphocyte induce at the same time and mutually stimulate maturity, thus simplifying operation steps, reducing experiment consumable items and shortening cell culturing time. In addition, normalized experiment process technology can be easily mastered, thus facilitating clinic popularization and application.

The invention discloses an open multifunctional test platform related to gas reaction. A gas source library comprises a plurality of gas sources. A gas distribution system comprises a plurality of mixing tanks and gas purging tanks which are connected with the gas sources through a plurality of mass flow meters, and the mixing tanks are connected with inlet ends of a reaction system through a plurality of flow distributors. The reaction system comprises a plurality of reactor assemblies connected with the flow distributors, wherein the reactor assemblies are installed on support or supportingbases, the outlet ends of the reactor assemblies are sequentially connected with a separating tank and a multi-channel switching valve through a pressure buffer tank, and the multi-channel switching valve makes the reactor assemblies connected in parallel or in series. A detecting and monitoring system comprises temperature probes, pressure meters and gas concentration detectors distributed in parts, matched display instruments and a heating controller. The shortcoming of low researching and testing efficiency of a traditional single set of equipment is overcome, and the problems of few gas paths, large batch-type testing work amount, long period and the like of an existing experimental device related to gas reaction are solved.

The invention relates to an economical quantitative non-invasive silicious dust-induced pulmonary fibrosis occupational disease mouse model establishing method and preparation device. The invention relates to processes of intubation needle manufacturing, mouse fixing device manufacturing, mouse tracheal intubation operation and the like in a silicosis fibrosis model. Specifically, each mouse is fixed on a self-made mouse fixing plate after being anesthetized, the glottis is exposed, and an SiO2 suspension is quantitatively dripped into the trachea of the mouse by using a self-made trachea cannula needle. Intubation is conducted once every 5-7 days for one month, and the silicosis fibrosis occupational disease model is successfully established. The lung tissue of the mouse is taken for lung morphology and pathology analysis, and the result proves that the method is simple to operate and low in cost, the success rate of the silicosis fibrosis occupational disease mouse model is high, and the fibrosis degree is progressive. The invention provides a good experimental animal model for further research on the pathogenesis of the silicious dust-induced pulmonary fibrosis and drug screening.

The invention discloses an RT-PCR (Reverse Transcription-Polymerase Chain Reaction) primer for detecting poultry-like type H1N1 swine influenza virus and a kit. The RT-PCR primer comprises two pairs: primers for H1 gene and primers for N1 genes, wherein the nucleotide sequence of an upstream primer Hu on the primers for the H1 gene is 5'-GACTCTAGATTTCCATGACCTT-3', and the nucleotide sequence of a downstream Hd is 5'-ACCCATTAGAACACATCCAGAA-3'; the nucleotide sequence of an upstream primer Nu on the primers for the N1 gene is 5'-TCCAAGGGGGATGTGTTTG-3', and the nucleotide sequence of a downstream primer Nd is 5'-GACCAAGCGACTGACTCAA-3'; the kit comprises the primer. The RT-PCR primer and the kit are adopted for detecting the poultry-like type H1N1 swine influenza virus and have the advantages of high sensitivity, strong specificity, fastness and convenience and the like.

The invention discloses a method for improving the repeatability of human albumin aluminum residue. The method for testing human blood albumin aluminum residue comprises the following steps: S1, solution preparation, wherein a diluent is prepared from 0.15% to 0.25% of a non-ionic surfactant solution, and a standard aluminum solution is prepared by the steps of preciously measuring 0.1ml of 100 micron / ml standard aluminum solution, placing in a 100ml volumetric flask and diluting with deionized water; S2, detection solution preparation, wherein 2.5ml of a diluent is taken as a blank solution,2.3ml of the diluent is added into 0.2ml of a test solution to obtain a test solution, and a standard aluminum plus test solution is prepared by the steps of adding 1.8ml of the diluent into 0.2ml ofthe test solution and 0.5ml of the standard aluminum solution; and S3, testing, namely with an atomic absorption spectrophotometer for testing, setting graphite furnace parameters, accurately measuring the blank solution, the test solution and the standard aluminum plus test solution, and injecting the solutions into the instrument. The method significantly increases the repeatability of human albumin aluminum residue.

The invention relates to a top cover type cleaning device. The top cover type cleaning device comprises a barrel body, a top cover and a hinged part, wherein a washing cavity is formed in the barrel body; the top cover is hinged to the upper portion of the washing cavity and is used for sealing the washing cavity; a spraying structure is formed on the side, facing the washing cavity, of the top cover, so that the washing device can carry out spraying from the top cover to the washing cavity, and the spraying area and the washing effect formed by the top spraying mode are far superior to thoseof a side spraying mode; one end of the hinged part is fixed with the barrel body, and the other end of the hinged part is fixed with the top cover; and the hinged part comprises a memory alloy hinge,and opening or closing of the top cover can be controlled by controlling opening and closing movement of the memory alloy hinge, so that the loss rate of parts is greatly reduced, automatic opening and closing of the top cover are guaranteed, and experimental consumables are reduced.

The invention relates to an automatic device for wave-shifted optical fiber bending. The device comprises a flat plate, wherein two ends of the flat plate are connected with bending plates which can only rotate by 90 degrees in a hinged mode correspondingly, the flat plate is provided with a first optical fiber groove group matching a wave-shifted optical fiber side by side, one sides of the bending plates are provided with bending handles correspondingly, the other sides of the bending plates are provided with pressing devices matching the wave-shifted optical fiber correspondingly, a bendingdevice is arranged on the side, not provided with the optical fiber groove group, of the flat plate and comprises a bending driver arranged on the flat plate, bending output shafts are arranged on the front side and the rear side of the bending driver correspondingly, the two bending output shafts are symmetrical with respect to the center of a center point of the flat plate, the bending output shafts are sleeved with bending rotating cylinders, bending pull ropes are fixed to the bending rotating cylinders, and the other ends of the bending pull ropes are arranged on pull rope installation bases on the side faces of the bending plates. According to the device, the optical fiber water bath bending consistency is better, the bending efficiency is higher, the bending reject ratio is reduced, and later experimental data is more accurate and controllable.

The invention discloses a screening method for 110 types of medicines in feed, which specifically includes the following steps: preparation of standard work solution, pre-analysis treatment of sample to be assayed, creation of database and qualitative analysis. By utilizing the high-sensitivity MSMS (Tandem Mass Spectrometry) function of a liquid chromatography-tandem quadrupole time-of-flight mass spectrometer, the screening method can accurately determine parent ions and daughter ions in medicines and rapidly determine added medicine ingredients, moreover, an assay method for a variety of antibacterial medicines in feed is also established, and for a variety of added antibacterial medicines in the feed industry in China, the screening method has a highly efficient, accurate and precise assay measure, and is at the advanced level in china at present. The screening method established by the invention greatly solves the phenomenon of arbitrary addition in the feed industry, the more precise and accurate assay method is perfected for the inspection department, more time is saved, most of experimental consumables are saved, and the invention makes an immeasurable contribution to food safety and human healthy life.

The invention discloses a plasma based object characterization detection method and device. Physical characteristics of the surface of a to-be-detected object are represented according to interactionforce between plasma and the to-be-detected object. The plasma is taken as a probe, when approaching the surface of the object, the plasma has interaction with the surface of the object, the contour of the surface of the object can be obtained by measuring influence of the interaction between the plasma and the surface of the object, and characterization detection for the object is realized.

The invention discloses equipment for observing a directional solidification process in real time. The equipment comprises a CCD micro-camera, a constant temperature water tank, a heating zone, a coldzone, a linear displacement table, a rubber pipe, a glass container, a heater, a heat insulation plate, a shock absorption plate, a computer, a cold light source, a glass plate, a connecting rod, a supporting rod, a temperature sensor I, a groove, a water inlet, a water outlet, a peristaltic pump, a temperature sensor II and a vacuum pump. The equipment for observing the directional solidification process in real time can directly observe the eutectic solidification of an alloy and the forming process of a porous material, in-depth study of a eutectic growth mechanism and a formation mechanism of the porous material can be researched, and thus the development of the material and condensed matter physics can be further promoted, and an eutectic and porous composite material and other materials with better performance are prepared.

The invention discloses a slicing machine. The slicing machine is characterized in that a workbench bracket is fixed to a workbench; the upper end of the workbench bracket is bent to be horizontal; acutter structure capable of vertically moving is arranged on the workbench bracket; and a cutting bearing structure right opposite to the cutter structure is arranged on the workbench. According to the slicing machine, the adopted cutter structure can be changed according to the requirement, a cutter is fixed through a circular ring and a pressing pillar, the cutter is bent through the circular ring and the pressing pillar, and the cutter has radian meeting the cutting requirement; the integral structure is simple, operation is convenient, practicability is high, multiple layers of raw materials can be cut at a time during cutting, and cutting efficiency is high; and the cutting specification is changed through changing of the circular ring, corner materials are cut, the utilization rate for materials is high, experiment consumables are saved, and production cost is reduced.

The invention discloses a six-hole plate for cell culture, wherein a collecting drainage groove (4) is formed on the box body (1) of the six-hole plate; a drainage opening (5) is formed at the port of the collecting drainage groove (4); a drainage groove (3) for connecting each culture hole (2) with the collecting drainage groove (4) is formed on the box body (1) of the six-hole plate; a watershed (7) which is higher than the bottom surface of the culture hole (2) is arranged in the drainage groove (3). The six-hole plate for cell culture is low in cost, easy to manufacture and convenient to use; the brand new design is realized based on the structure of the existing six-hole plate; the washing liquid can be removed efficiency and quickly due to the addition of the drainage groove and the collecting drainage groove beside the holes; as a result, the liquid exchange method of absorbing and draining the waste liquid hole by hole at present is changed and experimental steps are simplified; the experimenter can save time and labor; in the meantime, experimental consumables can be saved and misoperation caused by mixing suction tubes can be avoided; continuous washing by using flowing PBS (Phosphate Buffer Solution) can be realized and the washing efficiency is high.

The invention discloses a plasma based object characterization detection method and device. Physical characteristics of the surface of a to-be-detected object are represented according to interactionforce between plasma and the to-be-detected object. The plasma is taken as a probe, when approaching the surface of the object, the plasma has interaction with the surface of the object, the contour of the surface of the object can be obtained by measuring influence of the interaction between the plasma and the surface of the object, and characterization detection for the object is realized.

The invention discloses a method for determining the content of volatile fatty acid in ruminant rumen fluid. The method comprises the following steps: preparing a standard solution, setting headspace sampling conditions, setting gas chromatography conditions, treating a sample and the like. According to the present invention, the VFA content in the ruminant rumen fluid is determined by using the headspace gas chromatography, the sample pretreatment is not required, and the sample introduction analysis is directly performed by using the headspace bottle so as to furthest retain the original components, and the detection result is closer to the real components of the sample. Compared with common sample introduction analysis, pollution of sample impurities to the chromatographic column is effectively reduced, the maintenance cost is reduced, and meanwhile, experimental consumables used for sample pretreatment are saved.

The invention discloses an open multifunctional test platform related to gas reaction. A gas source library comprises a plurality of gas sources. A gas distribution system comprises a plurality of mixing tanks and gas purging tanks which are connected with the gas sources through a plurality of mass flow meters, and the mixing tanks are connected with inlet ends of a reaction system through a plurality of flow distributors. The reaction system comprises a plurality of reactor assemblies connected with the flow distributors, wherein the reactor assemblies are installed on support or supportingbases, the outlet ends of the reactor assemblies are sequentially connected with a separating tank and a multi-channel switching valve through a pressure buffer tank, and the multi-channel switching valve makes the reactor assemblies connected in parallel or in series. A detecting and monitoring system comprises temperature probes, pressure meters and gas concentration detectors distributed in parts, matched display instruments and a heating controller. The shortcoming of low researching and testing efficiency of a traditional single set of equipment is overcome, and the problems of few gas paths, large batch-type testing work amount, long period and the like of an existing experimental device related to gas reaction are solved.