Method for determining content of volatile fatty acid in ruminant rumen fluid

A technology of volatile fatty acids and ruminants, which is applied in the field of animal husbandry to achieve the effects of saving experimental consumables, reducing maintenance costs, and reducing pollution

Pending Publication Date: 2021-12-31
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no set of methods for determining the content of volatile fatty acids in rumen fluid of ruminants using this technology.

Method used

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  • Method for determining content of volatile fatty acid in ruminant rumen fluid
  • Method for determining content of volatile fatty acid in ruminant rumen fluid
  • Method for determining content of volatile fatty acid in ruminant rumen fluid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A method for determining the content of volatile fatty acids in rumen juice of ruminants, comprising the steps of:

[0033] S1, standard solution preparation

[0034] Accurately weigh the standard products of acetic acid, propionic acid, and butyric acid, and make up to volume with pure water to prepare a mixed standard stock solution of 320mmol / L acetic acid, 32mmol / L propionic acid, and 32mmol / L butyric acid for use; pipette different volumes respectively The standard stock solution is configured as a mixed standard solution with a concentration of 80%, 100% and 120% of the measured concentration of the sample;

[0035] S2, headspace sampling conditions

[0036] Headspace sampler conditions: Equilibrium temperature 85°C, sample flow path temperature 180°C, transfer line temperature 200°C, equilibration time 30min, injection time 1min;

[0037] S3, gas chromatography conditions

[0038] The chromatographic column is SH-Stabilwax quartz capillary column; the temperat...

Embodiment 2

[0051] The present embodiment provides a kind of concrete test method as follows:

[0052] A method for determining the content of volatile fatty acids in rumen juice of ruminants, comprising the steps of:

[0053] 1 Materials and methods

[0054] 1.1 Instruments and reagents

[0055]HSS 86.50 headspace sampler (DANI, Italy); GC-2010Plus gas chromatograph (Shimadzu, Japan), equipped with hydrogen flame ionization detector (FID); Eppendorf series pipettes; From Feihong Packaging Materials Factory (Nantong City, Jiangsu Province); Glacial acetic acid, propionic acid, and butyric acid are all chromatographically pure, purchased from Aladdin Company (Shanghai); ethanol, propanol, and butanol are all chromatographically pure, purchased from Bailingwei Company (Beijing); air, nitrogen, and hydrogen were purchased from Hangzhou Jingong Materials Co., Ltd.; ultrapure water was produced by NANO pure UV / UF ultrapure water system.

[0056] 1.2 Standard solution preparation

[0057] A...

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Abstract

The invention discloses a method for determining the content of volatile fatty acid in ruminant rumen fluid. The method comprises the following steps: preparing a standard solution, setting headspace sampling conditions, setting gas chromatography conditions, treating a sample and the like. According to the present invention, the VFA content in the ruminant rumen fluid is determined by using the headspace gas chromatography, the sample pretreatment is not required, and the sample introduction analysis is directly performed by using the headspace bottle so as to furthest retain the original components, and the detection result is closer to the real components of the sample. Compared with common sample introduction analysis, pollution of sample impurities to the chromatographic column is effectively reduced, the maintenance cost is reduced, and meanwhile, experimental consumables used for sample pretreatment are saved.

Description

technical field [0001] The invention relates to the technical field of animal husbandry, in particular to a method for measuring the content of volatile fatty acids in rumen fluid of ruminants. Background technique [0002] Volatile fatty acids, also known as short-chain fatty acids, are the main products of carbohydrates fermented by various microorganisms in the rumen of ruminants. The VFAs in ruminants are mainly acetic acid, propionic acid and butyric acid, accounting for 90% to 95% of the total VFA. The energy it provides accounts for about 2 / 3 of the total energy absorbed and utilized by ruminants. Therefore, it is of great significance to carry out the determination of VFA to study rumen fermentation of ruminants and improve feed nutrient absorption and energy conversion rate. [0003] At present, the detection methods of VFA mainly include gas chromatography, high performance liquid chromatography, atomic absorption spectrophotometry and capillary electrophoresis, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/68
CPCG01N30/02G01N30/06G01N30/68
Inventor 王俊红王艳明董伟仁张圆圆单颖王佳堃刘建新
Owner ZHEJIANG UNIV
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