RT-PCR (Reverse Transcription-Polymerase Chain Reaction) primer for detecting poultry-like type H1N1 swine influenza virus and kit

A technology of RT-PCR and swine influenza virus, applied in the field of RT-PCR primers and kits for detecting poultry-like H1N1 swine influenza virus, achieving good specificity, reducing detection cost, and avoiding false negative results

Inactive Publication Date: 2014-02-12
广西壮族自治区动物疫病预防控制中心
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, avian-like H1N1 swine influenza has not yet appeared in North American pig populations. This strain only continues to spread in Eurasia and has not been reported outside Eurasia. It is also known as Eurasian swine influenza H1NI virus

Method used

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  • RT-PCR (Reverse Transcription-Polymerase Chain Reaction) primer for detecting poultry-like type H1N1 swine influenza virus and kit
  • RT-PCR (Reverse Transcription-Polymerase Chain Reaction) primer for detecting poultry-like type H1N1 swine influenza virus and kit
  • RT-PCR (Reverse Transcription-Polymerase Chain Reaction) primer for detecting poultry-like type H1N1 swine influenza virus and kit

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Embodiment Construction

[0045] The present invention will be further described below in conjunction with embodiment, but do not limit protection scope and application scope of the present invention:

[0046] 1. Kit for detecting foot-and-mouth disease type A virus

[0047] A test kit for detecting avian-like H1N1 swine influenza virus, including the following reagents, see Table 2 for details, the primers are designed by the laboratory, and other reagents can be purchased from the market.

[0048] Table 2 Detection kits for avian-like H1N1 swine influenza virus

[0049]

[0050]

[0051] 2. How to use the kit

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Abstract

The invention discloses an RT-PCR (Reverse Transcription-Polymerase Chain Reaction) primer for detecting poultry-like type H1N1 swine influenza virus and a kit. The RT-PCR primer comprises two pairs: primers for H1 gene and primers for N1 genes, wherein the nucleotide sequence of an upstream primer Hu on the primers for the H1 gene is 5'-GACTCTAGATTTCCATGACCTT-3', and the nucleotide sequence of a downstream Hd is 5'-ACCCATTAGAACACATCCAGAA-3'; the nucleotide sequence of an upstream primer Nu on the primers for the N1 gene is 5'-TCCAAGGGGGATGTGTTTG-3', and the nucleotide sequence of a downstream primer Nd is 5'-GACCAAGCGACTGACTCAA-3'; the kit comprises the primer. The RT-PCR primer and the kit are adopted for detecting the poultry-like type H1N1 swine influenza virus and have the advantages of high sensitivity, strong specificity, fastness and convenience and the like.

Description

technical field [0001] The invention belongs to the technical field of molecular biological detection of animal viruses, and in particular relates to RT-PCR primers and a kit for detecting poultry-like H1N1 swine influenza virus. Background technique [0002] H1N1 subtype swine influenza virus (Swine influenza virus, SIV) is one of the important pathogens of swine influenza (Swine influenza, SI). A total of 10-11 proteins are encoded. The HIN1 subtype of swine influenza was first reported in the United States in 1918. Shope isolated and identified this subtype of virus for the first time in 1930. This was also the first confirmed swine influenza virus. Since then, it has spread continuously and has been found in five continents. The virus can infect pigs and cause acute and febrile respiratory infectious diseases in pigs. It is mainly characterized by sudden respiratory system symptoms and rapid recovery. It is one of the porcine respiratory syndrome syndrome diseases. Bec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/68C12N15/11C12R1/93
CPCC12Q1/70C12Q1/686C12Q2531/113
Inventor 颜健华熊毅冯淑萍孙翔翔李春英
Owner 广西壮族自治区动物疫病预防控制中心
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