The invention discloses a method for control and in-situ detection of enzymatic activity under
irradiation of electronic beams. The method includes the following steps of 1, preparing a
titanium dioxide thin-film field-effect tube; 2, carrying out
enzyme immobilization; 3, putting a device into an SEM sample table, and adopting
silicon as a gate
electrode, wherein any two adjacent electrodes in agold
electrode array are adopted as a source
electrode and a leakage electrode, and the electrodes are connected with aerial connectors on a cavity through leads; 4, putting the device of the electrodes into a
scanning electron microscope, and using the electronic beams for
radiation; 4, conducting a conductance testing. On the basis of the
titanium dioxide thin-film field-effect tube, enzymes arefixed to the field-effect tube, the electronic beams of the
scanning electron microscope are adopted as the
irradiation source, the electrodes of the field-effect tube are drawn to a
semiconductor property analysis instrument outside an SEM cavity through the leads and a cavity connection port for conducting in-situ conductance measurement, and the
enzyme activity is monitored through changes ofconductance. The generation of the electronic beams does not depend on any radioactive element, and in-situ monitoring of the
enzyme activity under the
irradiation of the electronic beams is achieved.