138results about How to "High purification yield" patented technology

The invention relates to a method for separating and purifying a panaxoside compound-K from microorganism zymocyte by applying macroporous resin for preparing a raw medicine. The purifying yield reaches more than 72 percent, and the content of the panaxoside compound-K in a product is more than 96 percent. The method mainly comprises the following steps of: adding ethanol into zymocyte for extracting and concentrating; decoloring by active carbon and filtering; after adding water into filter liquor for diluting, leading the filter liquor to pass through a macroporous resin chromatographic column; washing by a low-concentration organic solvent aqueous solution for removing pigment and high-polarity saponin; eluting by a high-concentration organic solvent aqueous solution to obtain a panaxoside compound-K component; and finally crystallizing to obtain the panaxoside compound-K raw medicine with the content of more than 96 percent. The invention can provide a great amount of qualified raw medicine samples for the pharmaceutical development of the panaxoside compound-K.

The invention belongs to the field of medicinal chemistry, and in particular relates to a preparation method of teduglutide. The preparation method of the invention is as below: respectively synthesizing a teduglutide sequence site No.4-33 peptide resin fragment and a teduglutide sequence No.1-3 peptide resin fragment through solid phase synthesis; then coupling the teduglutide sequence site No.4-33 peptide resin fragment and the teduglutide sequence No.1-3 peptide resin fragment by an appropriate resin vector to obtain a teduglutide resin; and finally cracking to obtain crude teduglutide, and purifying to obtain the pure teduglutide. The preparation method provided by the invention can effectively avoid side reaction such as degradation caused by an Asp-Gly structure of the No. 3-4 site, also can reduce the His1 racemization, thus effectively improving the purity of crude peptide and improve the purification yield. Compared with the prior art, the preparation method of the invention has the advanategs of high purity of the prepared teduglutide product, less by-product and high product yield, and is beneficial for mass production of teduglutide.

The invention discloses a purification method of echinocandins antifungal drug anidulafungi. The method comprises the following steps of: (1) preparing a dry sample: adding organic solvent in a crude product of the anidulafungin to dissolve the crude product, adding silica gel after the crude product of the anidulafungin is adequately dissolved, and uniformly mixing and drying the mixture to obtain the anidulafungin dry sample; (2) pressurizing and eluting: uniformly filling the anidulafungin dry samples in the top end of a chromatographic column with silica gel, pressurizing and eluting the chromatographic column with the anidulafungin dry samples by adding elution solvent, utilizing a high-effective liquid phase chromatography to monitor, and collecting the elution solution with the anidulafungin content being greater than 98 percent; and (3) concentrating: concentrating the elution solution with the anidulafungin content being greater than 98 percent until dryness to obtain a pure product with the anidulafungin content being greater than 98 percent. By adopting the column chromatography, simplicity in operation is realized, and the equipment cost is low; the organic solvent with low toxicity and low boiling point is adopted as the elution solution, so that the subsequent recycling treatment is simple, and the environmental pressure can be greatly reduced; and the purification separation time is short, the purification effect is good, the purification yield is high, and the purification method is applicable to industrialized mass production.

The invention relates to a recombinant human nerve growth factor purifying method based on a CHO cell expression system. The recombinant human nerve growth factor purifying method based on the CHO cell expression system comprises the following steps: 1), centrifugating supernatant, successively removing living cells and cell fragments, and removing impurities and bacteria by filtering; 2), applying a supernatant sample to an EMD SO3-(M) chromatography column, rinsing unbound protein and impure protein, eluting and collecting a target protein peak; 3), concentrating the protein with ultrafiltration equipment with a molecular weight cutoff of 3K; and 4), applying the sample to Superdex 75-containing chromatography column and collecting the target protein peak, namely a recombinant human nerve growth factor stoste. According to the CHO cell expression system and the recombinant human nerve growth factor purifying method based on the CHO cell expression system, the recombinant human nerve growth factor with the purity greater than 98% and the specific activity higher than the 5* 10 to the power of 5 AU / mg can be prepared; compared with other methods, the recombinant human nerve growth factor purifying method based on the CHO cell expression system has the advantages that the steps are reduced, the production cycle is greatly shortened, and the processing capacity and the yield are improved; therefore, the recombinant human nerve growth factor purifying method based on the CHO cell expression system is application for large-scale production.

The invention relates to a trelagliptin purification method. The method comprises heating and dissolving trelagliptin in a mixed solvent, carrying out crystallization and separating solids, wherein the mixed solvent comprises isopropanol and one of methyl acetate, acetonitrile and ethanol. The trelagliptin purification method realizes a high trelagliptin yield and high trelagliptin purity.

The invention relates to an acidic proteinase and a preparation method thereof and the acidic proteinase belongs to the microbial acidic proteinase. The invention is characterized in that 1) the enzymatic characteristics of the acidic proteinase are as follows: the optimum pH value is 2.5-3.5, the stable pH value is 2.5-6.0; the optimum temperature is 40-50 DEG C, the temperature stability range is 30-50 DEG C; and 2) the preparation method uses Aspergillus Niger which is stored in the China Center of Industrial Culture Collection (CICC) and has a preservation number of 2238, as the enzyme-producing strain and uses wheat bran as the raw material; the solid fermentation technology is adopted for preparation; the fermentation and enzyme-producing capability is not less than 47000u / g (dry yeast), the liquid enzyme yield is not less than 85% and the soid enzyme yield is not less than 80%, which all achieve the food grade sanitation standard. The invention provides the acidic proteinase and the preparation method thereof, wherein the utilization rate of the fermentation equipment is high, the byproduct of crop processing is used as the main raw material, the enzyme activity for fermentation is high, the extracting and purifying rate of enzyme is high and the production cost is low. The acidic proteinase of the invention is suitable to be used as the raw material or product additive of the products in the leather industry, the pharmaceutical industry, the brewing industry and the feed industry.

The present invention is affinity particles that are characterized by having phosphorylcholine groups represented by the following formula (1) covalently bonded onto the surface of inorganic powder and also by having ligands having specific affinity with a certain target substance covalently bonded or adsorbed onto the surface of inorganic powder. The object of the present invention is to provide an affinity separation method that uses affinity particles utilizing inexpensive inorganic particles and is capable of separating the target substance easily and with high accuracy.

The invention relates to adefovir dipivoxil monohydrate and a preparation method thereof. According to the preparation method, water-containing acetone is taken as a solvent and is subjected to freeze drying so as to generate freeze-dried powder of adefovir dipivoxil monohydrate. The preparation method is easy and convenient to operate, monohydrate can be selectively prepared, the yield of adefovir dipivoxil monohydrate is high, and the purity of adefovir dipivoxil monohydrate is good; the preparation method is very applicable to large-scale production.