67results about How to "Hard to quench" patented technology

The invention discloses fluorescent waterborne polyurethane based on chromophore in dihydric alcohol and a preparation method thereof. The fluorescent waterborne polyurethane is generated by utilizing the fluorescent dihydric alcohol containing the fluorescent chromophore to be reacted with diisocyanate and hydrophilic chain-extender. When the dihydric alcohol containing the fluorescent chromophore forms the fluorescent waterborne polyurethane latex, since the fluorescent chromophore is already chemically keyed into a polyurethane molecular chain, the fluorescent intensity is greatly enhanced, and a typical fluorescent enhanced effect can be displayed. The fluorescent chromophore is uniformly distributed in the polyurethane, the content is controllable, the chromophore is difficult to migrate, the fluorescence can be stored for a long time, the generated fluorescent waterborne polyurethane adopts the water as a solvent, so that safety in transportation and use can be realized, the environment is not polluted, only the low-boiling-point organic solvent such as acetone and butanone is used in the production process, and the solvent is easy to recover.

The invention belongs to the field of microorganism animal cell system, and relates to an establishment method of a fluorescence visualization human hepatoma nude mice model which can self emit high strength red or green fluorescences and has the metastatic ability. After obtaining a human high-metastatic fluorescence hepatoma cell line HCCLM3-R, HCCLM3-G, HCCLM6-R and HCCLM6-G of fluorescence genes with high chromosome conformable degree, high fluorescence intensity and stable expression by using the method of pseudotype slow virus infection, hepatoma cells expressed by the fluorescences is placed beneath nude mice skin to establish a fluorescence visualization high-metastatic human hepatoma nude mice subcutaneous tumor model or subcutaneous fluorescence expression tumor tissues is placed in nude mice hepar to establish a fluorescence visualization high-metastatic human hepatoma nude mice hepar primary tumor model. The got model can be used as a tracer of animal in vivo hepatoma cells, and can be used for molecular mechanism study of hepatoma recurrence and metastatic and prophase therapeutic effect discrimination for new anti-hepatoma therapy and new anti-hepatoma drugs, etc.

The invention discloses an immunofluorescent staining and interpretation method for circulating tumor cells. The method comprises the following steps: S10, performing centrifugal treatment on a blood sample diluted with a phosphoric acid buffer solution, and removing erythrocyte and leukocyte in the blood sample; S20, moistening the treated blood sample with the phosphoric acid buffer solution, adding a stationary liquid for immobilizing cells in the blood sample, adding a permeabilization liquid for permeabilization, and further adding a confining liquid for sufficient confining; and S30, dripping dye liquids of anti-pan-CK-FITC, anti-CD45-AF59 and anti-Vimentin-FITC into the treated blood sample at a dark place, performing light shielded incubation, removing the dye liquids, adding a nucleic acid fluorescent dye to continue incubation. The interpretation method comprises steps of scanning observation and result interpretation. Compared with the prior art, the dyeing method disclosed by the invention has the advantages of being simple and convenient to operate, easy to popularize and the like, and the interpretation method has the advantages of being accurate interpretation and the like.

The invention discloses a rare-earth carbon nanoparticle, a preparation method of the rare-earth carbon nanoparticle and application for determining a pH value based on a fluorescence color scale. Therare-earth carbon nanoparticle disclosed by the invention is a fluorescent nanoparticle formed by carrying out a one-pot hydrothermal reaction among rare-earth europium ion, terbium ion as well as 2,6-dipicolinic acid and polyethylene glycol 400. The fluorescence color scale principle of the rare-earth ions is changed to indicate the pH value by utilizing the enhanced luminescence effect of carbon dots and protonation of the 2,6-dipicolinic acid. The rare-earth carbon nanoparticle disclosed by the invention can give out light to indicate the pH value in the aqueous solution and / or cells, hasexcellent fluorescence in the aqueous solution, high pH determination sensitivity and fast pH response, and have advantages to determination of the pH value of a biological sample with excellent fluorescence background. Compared with the general visual colorimetry according to the fluorescence color scale, the method for determining the pH value based on the fluorescence color scale is accurate.

The invention discloses a fluorescent nano-particle based cell membrane imaging reagent which comprises a component A and a component B, wherein the component A adopts an amino-contained polymer whose side chain contains a biotin molecule unit and a hydrophobic anchoring unit; and the component B adopts avidin module grafted fluorescent nano-particles. The reagent is based on fluorescent nano-particles and a multi-site anchoring technology and has good biocompatibility and good imaging effect, and the fluorescent particles are on a cell membrane, are not endocytosed, are not prone to quenching after long-time laser radiation and can be used for long-time tracing labeling of the cell membrane.

The invention discloses a preparation method of carboxyl polystyrene copolymerized fluorescent microspheres, which is to put absolute ethanol, initiator azobisisobutyronitrile, and dispersant polyvinylpyrrolidone in water to obtain a clear and transparent solution, and inject the same into the solution at the same time Styrene, acrylic acid and allyl fluorescent dye are added, and the copolymerization reaction is carried out under the protection of nitrogen to obtain carboxyl polystyrene copolymerized fluorescent microspheres. The invention adopts a dispersion polymerization method, and the prepared carboxyl polystyrene copolymerized fluorescent microsphere has good monodispersity and stable fluorescent performance.

The invention provides an III-family nitride biological probe and a preparation method thereof, and belongs to the crossing field of semiconductor material preparation and biomedicine. III-V family materials with potential application prospects serve as basic materials of the probe, the component, thickness and group number of multiple groups of quantum well material are adjusted to obtain an adjustable quantum wire structure, the requirement that the light emitting intensity and wavelength can be adjusted within certain range can be met, and due to mature preparation technology, the biological probe is highly stable in an organism, tends not to quench, is long in service life, and is metabolic.

The invention discloses a ruthenium (II) complex, the structure of which is as follows:; also discloses the preparation method of the compound, including the steps: first use o-phenanthroline 5,6-dione and X-diketone as raw materials to prepare X- RPIP, and then use X‑RPIP and cis‑Ru(L)2Cl2 as raw materials to prepare the product. Alternatively, X-RPIP is first prepared from X-BrPIP and X-RPIP, and then X-RPIP and cis-Ru(L)2Cl2 are used as raw materials to prepare the product. Alternatively, the ruthenium precursor Ru(L)2Cl2 and X‑R'''PIP are reacted as raw materials to prepare Ru(L)2(X‑R'''PIP)2+, and then Ru(L)2( X‑R'''PIP) 2+, the product is prepared as raw material. The synthesis method of the present invention is simple, especially the synthesized Λ-[Ru(bpy)2(p-BEPIP)]2+ is non-toxic to living cells, transmembrane rapidly, the fluorescent color is strong and easy to observe and not easy to quench, and the fluorescence excitation and The emission bands do not overlap and can be enriched in the nucleus.

The invention discloses a preparation method of a fluorescent waterborne polyurethane emulsion based on chromophore in diisocyanate. The preparation method is characterized by comprising the following steps of: enabling monoamine or monohydric alcohol containing fluorescent chromophore to be reacted with isocyanate, or enabling diamine or dihydric alcohol containing the fluorescent chromophore to be reacted with diisocyanate with a symmetric structure to synthesize diisocyanate containing the fluorescent chromophore, and then combining the diisocyanate containing the fluorescent chromophore with common diisocyanate to partially or completely substitute for the common diisocyanate to prepare the fluorescent waterborne polyurethane. According to the preparation method disclosed by the invention, the hard segment content and type of the prepared fluorescent waterborne polyurethane and the content (2-40wt%) of the fluorescent chromophore are controllable by adjusting the types and proportions of the diisocyanate and a chain extender, and the prepared fluorescent waterborne polyurethane has the advantages that the fluorescent chromophore is uniformly distributed and difficult to migrate, the fluorescent intensity is good in durability, and improvement in terms of optical, thermal and certain functional characteristics can be achieved while the fluorescent property is achieved.

The present invention relates to an OLED material, which has any structure as shown in general formulas I-III. The OLED material provided by the invention is a compound centered on per-peridonne, with high T1 energy level, narrow band gap and shallow highest occupied molecular orbital (HOMO) energy level. Xanthene and xanthene are easily sublimated and stable in the air without oxidative decomposition. It has high thermal stability and high glass transition temperature in air. By introducing groups with relatively large steric hindrance, this type of luminescent material is not easy to crystallize and quench, and has good film-forming properties. As a preferred solution of the present invention, the OLED material is used as a hole-transporting material of the hole-transporting layer.