67results about How to "Hard to quench" patented technology

The invention discloses fluorescent waterborne polyurethane based on chromophore in dihydric alcohol and a preparation method thereof. The fluorescent waterborne polyurethane is generated by utilizing the fluorescent dihydric alcohol containing the fluorescent chromophore to be reacted with diisocyanate and hydrophilic chain-extender. When the dihydric alcohol containing the fluorescent chromophore forms the fluorescent waterborne polyurethane latex, since the fluorescent chromophore is already chemically keyed into a polyurethane molecular chain, the fluorescent intensity is greatly enhanced, and a typical fluorescent enhanced effect can be displayed. The fluorescent chromophore is uniformly distributed in the polyurethane, the content is controllable, the chromophore is difficult to migrate, the fluorescence can be stored for a long time, the generated fluorescent waterborne polyurethane adopts the water as a solvent, so that safety in transportation and use can be realized, the environment is not polluted, only the low-boiling-point organic solvent such as acetone and butanone is used in the production process, and the solvent is easy to recover.

The invention discloses application of rhodamine B derivatives, and relates to application of rhodamine B derivatives provided with intra-molecular spiral rings and used as cell lysosome fluorescent colorants. The rhodamine B derivatives are rhodamine B derivatives with intra-molecular five-member rings; and the rhodamine B derivatives can be used as the cell lysosome fluorescent colorants and used for high-selectivity and long-term stably labeled cell lysosome. The application has the advantages that: (1) the amino-containing rhodamine B derivatives with intra-molecular five-member rings can be used as lysosome fluorescent labeling substances of cells of different types; (2) the rhodamine B derivatives have long retention time in cells and high light stability; and (3) the rhodamine B derivatives can be used for labeling and tracking intra-molecular lysosome for long time.

The invention discloses a method for detecting early apoptosis of cells. A phosphatidylserin antibody marked with a fluorescent dye Alexa Fluor 488 and a nucleic acid fluorescent dye 7-AAD are matched to detect the cells together, wherein the phosphatidylserin antibody is used for identifying normal living cells and apoptotic cells, and the nucleic acid fluorescent dye is used for identifying early apoptotic cells and late apoptotic cells in the apoptotic cells. After the two fluorescent dyes, namely the Alexa Fluor 488 and the 7-AAD with different emission wavelengths are detected and analyzed through a flow cytometry, the cells are divided into four subsets, wherein the Alexa Fluor 488+ / 7-AAD- subset is the early apoptotic cells.

The invention discloses a ruthenium (II) complex, of which the structure is as shown in the specification, and also discloses a preparation method of the complex. The method comprises the following steps: firstly, preparing X-RPIP from phenanthroline 5,6-diketone as a raw material, and then preparing a product, namely the ruthenium (II) complex from the X-RPIP and cis-Ru(L)2Cl2 as the raw materials; or firstly, preparing the X-RPIP from X-BrPIP as a raw material, and then preparing the product from the X-RPIP and cis-Ru(L)2Cl2 as the raw materials; or firstly, preparing Ru(L)2(X-R'''PIP)<2+> from Ru(L)2Cl2 and X-R'''PIP as the raw materials, and then preparing the product from the Ru(L)2(X-R'''PIP)<2+> as the raw material. The synthetic method disclosed by the invention is simple, especially the synthetic lambda-[Ru(bpy)2(p-BEPIP)]<2+> is free of toxicity on a living cell, quick to stride across a film, strong in fluorescence color, easy to observe, and not easy to quench, fluorescence excitation does not overlap with an emission waveband, and cell nucleus enrichment can be carried out.

The invention discloses a preparation method of fluorescent waterborne polyurethane acrylate. According to the preparation method, a free radical copolymerization reaction between an acrylate small-molecule fluorescent compound and a polyurethane prepolymer which is blocked by a blocking agent containing double bonds and is terminated with an NCO group is carried out such that a fluorescent chromophore is bonded to a polyurethane molecular chain in the form of a chemical bond. Then, the fluorescent waterborne polyurethane acrylate is obtained. According to the fluorescent waterborne polyurethane acrylate prepared by the above method, fluorophore addition can be regulated and controlled according to needs. In addition, fluorophore is not easy to migrate, and the fluorescence property is maintained enduringly. As the fluorophore generally contains a rigid structure with high steric hindrance, the fluorophore also has an effect of regulating optical property, thermal property, mechanical property and the like of waterborne polyurethane and some functional characteristics during copolymerization with polyurethane while the fluorescent waterborne polyurethane is obtained.

The invention discloses a preparation method of a perovskite quantum dot composite, comprising the following steps: S1, mixing a first precursor and a second precursor with an organic solvent to obtain a perovskite precursor mixed liquid; S2, mixing the perovskite precursor mixed liquid in S1 with polymer microspheres to obtain a mixture of perovskite quantum dot polymer microspheres; S3, and subjecting the mixture of perovskite quantum dot polymer microspheres in S2 to solvent removal treatment to obtain the perovskite quantum dot composite. The preparation method of the perovskite quantum dot composite is simple, rapid, efficient and easy to operate, and can produce a perovskite quantum dot composite which is stable in air, is not easily quenched during light emitting, and can be furtherdirectly applied.

The invention belongs to the field of microorganism animal cell system, and relates to an establishment method of a fluorescence visualization human hepatoma nude mice model which can self emit high strength red or green fluorescences and has the metastatic ability. After obtaining a human high-metastatic fluorescence hepatoma cell line HCCLM3-R, HCCLM3-G, HCCLM6-R and HCCLM6-G of fluorescence genes with high chromosome conformable degree, high fluorescence intensity and stable expression by using the method of pseudotype slow virus infection, hepatoma cells expressed by the fluorescences is placed beneath nude mice skin to establish a fluorescence visualization high-metastatic human hepatoma nude mice subcutaneous tumor model or subcutaneous fluorescence expression tumor tissues is placed in nude mice hepar to establish a fluorescence visualization high-metastatic human hepatoma nude mice hepar primary tumor model. The got model can be used as a tracer of animal in vivo hepatoma cells, and can be used for molecular mechanism study of hepatoma recurrence and metastatic and prophase therapeutic effect discrimination for new anti-hepatoma therapy and new anti-hepatoma drugs, etc.

The invention discloses a method for preparing a carboxyl polystyrene copolymerized fluorescent microsphere. The method comprises steps of putting absolute ethyl alcohol, initiator azodiisobutyronitrile and dispersing agent polyvinylpyrrolidone into water so as to obtain clear transparent solution, adding styrene, acrylic acid and allyl fluorescent dyes into the solution simultaneously, and copolymerizing under nitrogen protection so as to obtain the carboxyl polystyrene copolymerized fluorescent microsphere. The method adopts a dispersion polymerization manner; the prepared carboxyl polystyrene copolymerized fluorescent microsphere has good monodispersity and stable fluorescence.

The invention discloses an immunofluorescent staining and interpretation method for circulating tumor cells. The method comprises the following steps: S10, performing centrifugal treatment on a blood sample diluted with a phosphoric acid buffer solution, and removing erythrocyte and leukocyte in the blood sample; S20, moistening the treated blood sample with the phosphoric acid buffer solution, adding a stationary liquid for immobilizing cells in the blood sample, adding a permeabilization liquid for permeabilization, and further adding a confining liquid for sufficient confining; and S30, dripping dye liquids of anti-pan-CK-FITC, anti-CD45-AF59 and anti-Vimentin-FITC into the treated blood sample at a dark place, performing light shielded incubation, removing the dye liquids, adding a nucleic acid fluorescent dye to continue incubation. The interpretation method comprises steps of scanning observation and result interpretation. Compared with the prior art, the dyeing method disclosed by the invention has the advantages of being simple and convenient to operate, easy to popularize and the like, and the interpretation method has the advantages of being accurate interpretation and the like.

The invention discloses a rare-earth carbon nanoparticle, a preparation method of the rare-earth carbon nanoparticle and application for determining a pH value based on a fluorescence color scale. Therare-earth carbon nanoparticle disclosed by the invention is a fluorescent nanoparticle formed by carrying out a one-pot hydrothermal reaction among rare-earth europium ion, terbium ion as well as 2,6-dipicolinic acid and polyethylene glycol 400. The fluorescence color scale principle of the rare-earth ions is changed to indicate the pH value by utilizing the enhanced luminescence effect of carbon dots and protonation of the 2,6-dipicolinic acid. The rare-earth carbon nanoparticle disclosed by the invention can give out light to indicate the pH value in the aqueous solution and / or cells, hasexcellent fluorescence in the aqueous solution, high pH determination sensitivity and fast pH response, and have advantages to determination of the pH value of a biological sample with excellent fluorescence background. Compared with the general visual colorimetry according to the fluorescence color scale, the method for determining the pH value based on the fluorescence color scale is accurate.

The invention discloses a sensing material for concentration and temperature. The sensing material is an annular rare earth complex with a molecular formula of {[Ln(L1)3](L2O2)}2, wherein, Ln is a trivalent rare earth ion except for La<3+>, Gd<3+> and Lu<3+>, L1 is a beta-dione, and L2 is an aromatic conjugate compound. The sensing material for concentration and temperature can be reused for times, and the spectrum performance of the sensing material can steadily reappear.

The invention relates to a preparation method of fluorescence antibody for detecting a Newcastle disease virus and a solid-phase immunofluorescence detection assay kit. The preparation method comprises the following steps of: respectively deriving R-phycoerythrin (RPE) and an antibody resisting the Newcastle disease virus (NDV) by using a cross-linking agent SPDP (N-succinimidyl-3-(2-pyridyldithiol) propionate), cross-linking the derivatives in a proper molar ratio, and purifying through HPLC (High Performance Liquid Chromatography) to prepare an RPE marked NDV fluorescence antibody. The solid-phase immunofluorescence detection assay kit is formed from the fluorescence antibody, an NDV-resisting antibody, an agarose microsphere, diluted hydrochloric acid and a washing liquid. The assay kit comprises the following detection flows of: coating an activated microsphere with the antibody, washing, combining with a sample to be measured, washing, combining with the fluorescence antibody, fully washing, exciting by blue and green light in a fluorescence microscope, observing and judging the result. The prepared fluorescence antibody has the advantages of high yield, high purity, bright orange fluorescence and good stability; and the assay kit has signal enrichment action by using a spherical carrier and can increase detection sensitivity. The invention is applicable to the rapid detection of the Newcastle disease virus.

The invention discloses a fluorescent nano-particle based cell membrane imaging reagent which comprises a component A and a component B, wherein the component A adopts an amino-contained polymer whose side chain contains a biotin molecule unit and a hydrophobic anchoring unit; and the component B adopts avidin module grafted fluorescent nano-particles. The reagent is based on fluorescent nano-particles and a multi-site anchoring technology and has good biocompatibility and good imaging effect, and the fluorescent particles are on a cell membrane, are not endocytosed, are not prone to quenching after long-time laser radiation and can be used for long-time tracing labeling of the cell membrane.

The invention discloses a preparing method for carboxylation porous crosslinked polystyrene copolymerized fluorescent microspheres. The preparing method is characterized by including the following steps of polystyrene seed sphere preparing, seed sphere activating, seed sphere swelling, polymerizing and pore-forming agent extracting. Compared with the prior art, the preparing method for the carboxylation porous crosslinked polystyrene copolymerized fluorescent microspheres has the advantages that the carboxylation porous crosslinked polystyrene copolymerized fluorescent microspheres with blue fluorescence, green fluorescence and red fluorescence are obtained; fluorochrome is evenly distributed in the microspheres, and therefore the high whole luminous efficiency of the microspheres is guaranteed; the fluorochrome stably exists in the microspheres, leakage and cancellation are not prone to occurrence, and pollution on test samples can be avoided; the grain size of the fluorescent microspheres prepared with the method is uniform, and is 7.5 microns, the specific area is large, and the good monodispersity is achieved; the light intensity is even in distribution; the preparing cost is low.

The invention discloses a preparation method of a near-ultraviolet polystyrene copolymerization fluorescence microsphere, and the preparation method is as follows: putting initiator azodiisobutyronitrile and dispersing agent polyvinylpyrrolidone in absolute ethanol to obtain a clear and transparent solution, simultaneously adding styrene and N-allyl carbazole to the solution for copolymerization reaction under the protection of nitrogen to obtain a polystyrene copolymerization fluorescence microsphere, and then introducing sulfonic acid groups or hydroxyl groups onto the surface of the polystyrene copolymerization fluorescence microsphere to produce the functional near-ultraviolet polystyrene copolymerization fluorescence microsphere. The preparation method adopts a method of dispersion polymerization to prepare the near-ultraviolet polystyrene copolymerization fluorescence microsphere having good monodispersity and stable fluorescent properties.

The invention relates to a method for preparing a fluorescent antibody for detecting avian influenza virus and a solid phase immunofluorescence detection kit. According to the invention, an avian influenza virus antibody is marked by allophycocyanin (APC) so as to prepare the avian influenza virus fluorescent antibody; the APC and the antibody are derived by a chemical cross-linking agent SPDP respectively; the derivatives are subjected to liquid phase cross-linking in a proper molar ratio; and then the fluorescent antibody is prepared by high pressure liquid chromatography purification. The solid phase immunofluorescence detection kit comprises the fluorescent antibody, CNBr activated agarose microspheres, the avian influenza virus antibody, cleaning solution and the like. The using method of the kit comprises the following steps of: activating a microsphere carrier and then coating the activated microsphere carrier by using the avian influenza virus antibody; cleaning; combining the microspheres coated with the antibody with a sample (an antigen) to be detected; combining the microspheres coated with the antibody with the fluorescent antibody after cleaning; removing the uncombined fluorescent antibody by cleaning; and observing under a fluorescence microscope and determining the result. The fluorescent antibody prepared by the method is characterized by high cross-linking efficiency, high purity, bright red fluorescence and stable performance. The microsphere solid phase carrier which is adopted by the kit can obviously improve fluorescent detection sensitivity, and is suitable for the quick detection of the avian influenza virus.

The invention discloses a method for preparing a nano fluorescent probe with high bio-safety. Fluorescent protein is wrapped by using a natural macromolecular chitosan material with extremely high bio-safety to improve the stability of the fluorescent protein and expand the using field and range of the fluorescent protein. The preparation method comprises the steps of adsorption and cross-linking. The method is quite simple and feasible and extremely high in wrapping efficiency; and the obtained nano particles have high stability, dispersibility and bio-safety, can protect the protein from being hydrolyzed by enzymes and denatured, and also have excellent performance of protecting the fluorescence from being quenched and blanched. In-vitro experiments show that the material can successfully enter cells and is applied to molecule level as the fluorescent probe with high bio-safety.

The invention discloses a dendrobium polysaccharide fluorescent marker for near-infrared imaging and a synthesis method thereof, wherein the dendrobium polysaccharide fluorescent marker near-infraredimaging is of a structure as shown in the formula (II). The dendrobium polysaccharide fluorescent marker and the synthesis method have the advantages that through improvement in the specific structureof a dendrobium polysaccharide derivative, the dendrobium polysaccharide derivative for near-infrared imaging can be obtained; through the corresponding design of the overall process flow of the synthesis method, reaction conditions and parameters of each step are controlled, so that the obtained dendrobium polysaccharide derivative can particularly serve as the dendrobium polysaccharide fluorescent marker for near-infrared imaging, and the problem that natural plant polysaccharides often need to be marked and traced for in-vivo and in-vitro experiments can be effectively solved. (The formula(II) are shown in the description.).

The invention relates to a preparation method for biocompatible quantum dots (QDs) and application of the QDs and belongs to the technical field of cell labeling. The technical scheme of the invention is as follows: cadmium selenide (CdSe) is firstly synthesized by adopting a water-phase method, and CdSe / ZnS core-shell-type QDs with low cytotoxicity are prepared through growing zinc sulfide (ZnS) on the surface of CdSe. An amphoteric polymer is synthesized and then is coupled to CdSe / ZnS so as to obtain CdSe / ZnS-Polymer with high water solubility and good biocompatibility. Amphoteric polymers coupled QDs have high water solubility and good biocompatibility, so that the condition that the QDs are used for carrying out fluorescence labeling on living cells and carrying out in-vivo tracing becomes possible. The biocompatible QDs are used for carrying out stem cell labeling, and fluorescence imaging is carried out on the in-vivo distribution of the QDs, so that the fluorescence signal is enhanced, and the sensitivity of observation is improved.

The invention discloses yellow fluorescent protein and application thereof. According to the invention, the wild-type red fluorescent protein DsRed2 is transformed so as to obtain the novel yellow fluorescent protein with amino acid mutation. The prokaryotic expression of the novel yellow fluorescent protein can achieve color development in a short time, is obvious in color development, is stablein color development in eukaryotic expression, is not easy to be quenched by incident light, and can be applied to fluorescent color development of various reporting systems and expression positioningof target proteins.

The invention discloses aggregation-induced fluorescent particles, a compound, multi-mode immunochromatography test paper and a preparation method of the multi-mode immunochromatography test paper. The aggregation-induced fluorescence particle is of a core-shell structure under a transmission electron microscope, has the particle size of 50-500nm, and is mainly composed of an aggregation-induced fluorescence material with color and fluorescence characteristics and a polymer. The aggregation-induced fluorescence particles are prepared by adopting a miniemulsion-solvent evaporation method, the equipment and the process are simple, the prepared composite particles are uniform in particle size, controllable in condition and good in repeatability, the concentration of the aggregation-induced fluorescence material in the particles is easy to control, and most importantly, the obtained particles have two characteristics of color and fluorescence. An immunochromatography test strip prepared by taking the particles as a signal carrier can be used for qualitative detection through naked eyes and can also be used for quantitative detection through fluorescence.

The invention discloses a preparation method of carboxyl polystyrene copolymerized fluorescent microspheres, which is to put absolute ethanol, initiator azobisisobutyronitrile, and dispersant polyvinylpyrrolidone in water to obtain a clear and transparent solution, and inject the same into the solution at the same time Styrene, acrylic acid and allyl fluorescent dye are added, and the copolymerization reaction is carried out under the protection of nitrogen to obtain carboxyl polystyrene copolymerized fluorescent microspheres. The invention adopts a dispersion polymerization method, and the prepared carboxyl polystyrene copolymerized fluorescent microsphere has good monodispersity and stable fluorescent performance.

The invention provides an III-family nitride biological probe and a preparation method thereof, and belongs to the crossing field of semiconductor material preparation and biomedicine. III-V family materials with potential application prospects serve as basic materials of the probe, the component, thickness and group number of multiple groups of quantum well material are adjusted to obtain an adjustable quantum wire structure, the requirement that the light emitting intensity and wavelength can be adjusted within certain range can be met, and due to mature preparation technology, the biological probe is highly stable in an organism, tends not to quench, is long in service life, and is metabolic.

The invention relates to an amino-modified water-soluble fluorescent quantum dot and a preparation method thereof. The water-soluble fluorescent quantum dot is of a multi-layer core-shell structure, an oil-soluble fluorescent quantum dot (such as CdSe / ZnS) is adopted as a core, the surface of the oil-soluble fluorescent quantum dot is subjected to a silanization rection, so that the lipophilic and hydrophobic properties of the quantum dots are modified into hydrophily in one step; furthermore, an amino group (-NH2) is adopted, so that surface amino modification for the water-soluble quantum dot is finished; the prepared amino-modified water-soluble quantum dot has an average size of 10nm and is uniform in size, has a wide excitation spectrum continuously distributed in a range of 250-602nm, and has good emission spectrum monochromaticity with a maximum emission wavelength of 634nm and fluorescence-emission half peak width of 43nm. The amino-modified quantum dot is good in biocompatibility, can be coupled with various bioactive substances, and has great practical value and application prospect in the biology and medicine fields such as immunofluorescence diagnose, cell imaging and living imaging.

The invention relates to a fusarium moniliforme SiC quantum dot fluorescence labeling method and particularly relates to a fluorescence labeling method for fusarium moniliforme SiC in different growth stages by cytotoxicity-free aqueous phase silicon carbide quantum dots, and a long-time-period fluorescence imaging technology for living cells. The method comprises the following steps of preparing a mixed solution of slant strain fusarium moniliforme and a PD culture medium; preparing a mixed culture medium of a mycete liquid-SiC quantum dot aqueous phase solution from the mixed solution and an SiC quantum dot aqueous phase solution at a volume ratio of 3:1; performing constant-temperature shaking culture on the mixed culture medium at a temperature of 28 DEG C and a speed of 200r / min for 3-10 days, thereby obtaining a fusarium moniliforme SiC quantum dot fluorescent mark; applying the mark to seedling root dip dyeing, thereby realizing dynamic monitoring, tracing and fluorescence imaging of a process that the fusarium moniliforme infects plant roots. The method is simple and effective, is low in cost and is capable of realizing fluorescence labeling of the fusarium moniliforme in different periods.

The invention discloses a preparation method of a fluorescent waterborne polyurethane emulsion based on chromophore in diisocyanate. The preparation method is characterized by comprising the following steps of: enabling monoamine or monohydric alcohol containing fluorescent chromophore to be reacted with isocyanate, or enabling diamine or dihydric alcohol containing the fluorescent chromophore to be reacted with diisocyanate with a symmetric structure to synthesize diisocyanate containing the fluorescent chromophore, and then combining the diisocyanate containing the fluorescent chromophore with common diisocyanate to partially or completely substitute for the common diisocyanate to prepare the fluorescent waterborne polyurethane. According to the preparation method disclosed by the invention, the hard segment content and type of the prepared fluorescent waterborne polyurethane and the content (2-40wt%) of the fluorescent chromophore are controllable by adjusting the types and proportions of the diisocyanate and a chain extender, and the prepared fluorescent waterborne polyurethane has the advantages that the fluorescent chromophore is uniformly distributed and difficult to migrate, the fluorescent intensity is good in durability, and improvement in terms of optical, thermal and certain functional characteristics can be achieved while the fluorescent property is achieved.

The present invention relates to an OLED material, which has any structure as shown in general formulas I-III. The OLED material provided by the invention is a compound centered on per-peridonne, with high T1 energy level, narrow band gap and shallow highest occupied molecular orbital (HOMO) energy level. Xanthene and xanthene are easily sublimated and stable in the air without oxidative decomposition. It has high thermal stability and high glass transition temperature in air. By introducing groups with relatively large steric hindrance, this type of luminescent material is not easy to crystallize and quench, and has good film-forming properties. As a preferred solution of the present invention, the OLED material is used as a hole-transporting material of the hole-transporting layer.

The invention provides a preparation method of a rural experience type light-storing luminescent material. The preparation method comprises the following specific steps: dehydrating a mixed solution of dihydric alcohol and trihydric alcohol at 100-110 DEG C under the vacuum condition of 0.05-0.2 MPa for 3-5 hours; adding an aliphatic ketone solution and an organic tin solution into the dehydratedalcohol solution, mixing, heating to 70-80 DEG C, adding triphenylmethane triisocyanate, and carrying out heat preservation reaction for 1.5-2.5 hours at 70-80 DEG C; and adding the light-storing luminescent material into the catalytic reaction, and continuing the heat preservation reaction to obtain the single-component light-storing polyurethane luminescent material. According to the luminescentmaterial prepared by the method, the light-storing and light-emitting functions of the coating are improved, the problems of the traditional fluorescent coating are solved, and the obtained luminescent material has the advantages of increased light-emitting stable strength, difficulty in migration, lasting and stable maintenance of a light-emitting group, excellent glossiness and weather resistance, difficulty in quenching and typical light-storing and light-emitting enhancement effect.