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Multi-mode aggregation-induced fluorescence immunochromatography test paper and preparation method thereof

A technology with aggregation-induced fluorescence and fluorescence characteristics, applied in the field of rapid immunoassay, it can solve problems such as limitations, and achieve the effects of stable markers, controllable conditions, and uniform particle size.

Pending Publication Date: 2022-07-22
SHANGHAI DERMATOLOGY HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing AIE materials used to prepare fluorescent microspheres mainly use the fluorescence characteristics of AIE materials to prepare fluorescent immunochromatography test paper, which limits its application in on-site naked-eye detection.

Method used

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  • Multi-mode aggregation-induced fluorescence immunochromatography test paper and preparation method thereof
  • Multi-mode aggregation-induced fluorescence immunochromatography test paper and preparation method thereof
  • Multi-mode aggregation-induced fluorescence immunochromatography test paper and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1: Preparation of aggregation-induced fluorescent particles and method for labeling C-reactive protein antibody:

[0035] A. Preparation of oil phase components: Disperse tetrastyrene-based AIE derivatives and polystyrene-maleic anhydride copolymer (molecular weight 1900, purchased from Sigma-Aldrich) in chloroform solution, and tetrastyrene-based AIE derivatives The concentration is 10 mg / mL, and the concentration of the polymer is 10 mg / mL;

[0036] B. Preparation of water phase components: Sodium dodecyl sulfonate and Triton X-100 are prepared into an aqueous solution, the mass concentration of sodium dodecyl sulfonate is 0.1%, and the mass concentration of Triton X-100 is 0.1%. is 0.5%;

[0037] C. Add the oil phase to the aqueous phase solution, stir magnetically until it is uniform, and then use an ultrasonic crusher to perform ultrasonic treatment on the mixed solution for 2 minutes to obtain a uniform and stable microemulsion;

[0038] D. At room tempera...

Embodiment 2

[0043] Example 2: Preparation of aggregation-induced fluorescent particles and method for labeling goat anti-rabbit immunoglobulin (IgG) antibody:

[0044] A. Preparation of oil phase components: Disperse tetrastyrene AIE derivatives and polystyrene-acrylic acid copolymer (molecular weight 37000, purchased from Sigma-Aldrich) in chloroform solution, the concentration of tetrastyrene AIE derivatives is 10mg / mL, the concentration of the polymer is 10mg / mL;

[0045] B, preparation water phase component, is mixed with sodium dodecyl sulfonate into aqueous solution, and the mass concentration of sodium dodecyl sulfonate is 0.5%;

[0046] C. Add the oil phase to the aqueous phase solution, stir magnetically until it is uniform, and then use an ultrasonic breaker to perform ultrasonic treatment on the mixed solution for 5 minutes to obtain a uniform and stable microemulsion;

[0047] D. At room temperature, the microemulsion was placed in an open container, and under magnetic stirri...

Embodiment 3

[0052] Example 3: Detection of C-reactive protein samples

[0053] Aggregation-inducing fluorescent particles labeled with C-reactive protein antibody in Example 1 (ie, aggregation-inducing fluorescent particles-C-reactive protein antibody complexes) and goat anti-rabbit IgG antibody-labeled aggregation-inducing particles in Example 2 (ie, aggregation-induced fluorescent particles) were used. Fluorescent particle-goat anti-rabbit IgG antibody complex) to prepare highly sensitive C-reactive protein aggregation-induced fluorescent particle multimodal immunochromatographic test paper, and the test paper production steps are as follows:

[0054] The aggregation-induced fluorescent particle-C-reactive protein antibody complex and the aggregation-induced fluorescent particle-goat anti-rabbit IgG antibody complex were coated on the coupling pad for use; the C-reaction was adjusted with phosphate buffered saline (PBS), respectively The concentration of another strain of protein and an...

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Abstract

The invention discloses aggregation-induced fluorescent particles, a compound, multi-mode immunochromatography test paper and a preparation method of the multi-mode immunochromatography test paper. The aggregation-induced fluorescence particle is of a core-shell structure under a transmission electron microscope, has the particle size of 50-500nm, and is mainly composed of an aggregation-induced fluorescence material with color and fluorescence characteristics and a polymer. The aggregation-induced fluorescence particles are prepared by adopting a miniemulsion-solvent evaporation method, the equipment and the process are simple, the prepared composite particles are uniform in particle size, controllable in condition and good in repeatability, the concentration of the aggregation-induced fluorescence material in the particles is easy to control, and most importantly, the obtained particles have two characteristics of color and fluorescence. An immunochromatography test strip prepared by taking the particles as a signal carrier can be used for qualitative detection through naked eyes and can also be used for quantitative detection through fluorescence.

Description

technical field [0001] The invention belongs to the technical field of rapid immunodetection, and in particular relates to an aggregation-induced fluorescent particle, a complex, a multi-mode chromatography test paper and a preparation method thereof Background technique [0002] Due to the advantages of simplicity, rapidity and low cost, immunochromatographic test strips are the main technical means for rapid on-site detection and analysis, and are widely used in clinical disease, food safety, environmental monitoring and other technical fields. The basic principle is that the nitrocellulose membrane is used as the chromatographic reaction membrane. The analyte to be tested in the sample has a specific immune reaction with the antigen or antibody fixed in the detection area on the membrane, and the signal intensity of the immune marker in the detection area is analyzed to achieve Detection of the analyte to be measured. Traditional immunochromatography uses colloidal gold ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/533
CPCG01N33/533
Inventor 张鹏飞范凌志严万年
Owner SHANGHAI DERMATOLOGY HOSPITAL
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