Preparation method for biocompatible quantum dots (QDs) and application of QDs

A technology of biocompatibility and quantum dots, which is applied in the field of preparation of biocompatible quantum dots, can solve the problems of poor water solubility and strong cytotoxicity of QDs, and achieve the effects of not being easy to quench, reducing cytotoxicity and high repeatability

Inactive Publication Date: 2016-02-24
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The purpose of the present invention is to overcome the current problems such as poor water solubility and strong cytotoxicity of QDs, and to provide a preparation method for biocompatible QDs. application in technology

Method used

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  • Preparation method for biocompatible quantum dots (QDs) and application of QDs
  • Preparation method for biocompatible quantum dots (QDs) and application of QDs
  • Preparation method for biocompatible quantum dots (QDs) and application of QDs

Examples

Experimental program
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Effect test

Embodiment 1

[0046] Embodiment 1: Preparation of CdSe / ZnS-Polymer

[0047] 1. Synthesis of CdSe: Under the protection of nitrogen, 39.5mg of selenium (Se) powder was dissolved in 50mL of liquid paraffin, and the temperature was gradually increased to 200°C. After vigorous stirring for 1 hour, the Se mixture was cooled to about 100°C. Dissolve 0.64g of CdO and 5.7g of stearic acid in 5ml of liquid paraffin, and stir at 160°C under nitrogen protection. When the mixture turns bright yellow, quickly inject the previously synthesized Se mixture and rapidly increase the temperature of the mixture to 200°C. After 90 minutes, the temperature of the mixture was lowered to 60°C, and 50 mL of chloroform was added until thoroughly mixed. The mixture was centrifuged at 8000 rpm for 10 minutes, and the supernatant was discarded. Add 50 mL of ethanol and mix well. After centrifugation again, the pellet was washed again with ethanol. The precipitate was dried and stored in a dark environment to obtai...

Embodiment 2

[0055] Embodiment 2: Preparation of CdSe / ZnS-Polymer

[0056] The difference between Example 2 and Example 1 is that in Step 1, under the protection of nitrogen, 79mg of selenium (Se) powder was dissolved in 50mL of liquid paraffin, and the temperature was gradually increased to 200°C. All the other steps are the same as in Example 1.

Embodiment 3

[0057] Embodiment 3: Preparation of CdSe / ZnS-Polymer

[0058] The difference between Example 2 and Example 1 is that in step 1, under the protection of nitrogen, 158mg of selenium (Se) powder was dissolved in 50mL of liquid paraffin, and the temperature was gradually increased to 200°C. All the other steps are the same as in Example 1.

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Abstract

The invention relates to a preparation method for biocompatible quantum dots (QDs) and application of the QDs and belongs to the technical field of cell labeling. The technical scheme of the invention is as follows: cadmium selenide (CdSe) is firstly synthesized by adopting a water-phase method, and CdSe / ZnS core-shell-type QDs with low cytotoxicity are prepared through growing zinc sulfide (ZnS) on the surface of CdSe. An amphoteric polymer is synthesized and then is coupled to CdSe / ZnS so as to obtain CdSe / ZnS-Polymer with high water solubility and good biocompatibility. Amphoteric polymers coupled QDs have high water solubility and good biocompatibility, so that the condition that the QDs are used for carrying out fluorescence labeling on living cells and carrying out in-vivo tracing becomes possible. The biocompatible QDs are used for carrying out stem cell labeling, and fluorescence imaging is carried out on the in-vivo distribution of the QDs, so that the fluorescence signal is enhanced, and the sensitivity of observation is improved.

Description

technical field [0001] The invention belongs to the technical field of cell labeling, and in particular relates to a preparation method and application of biocompatible quantum dots. Background technique [0002] In the research of cell biology, in order to observe structures and molecules of interest, the most common method is to label them with fluorescent light. However, traditional fluorescent probes suffer from many limitations in the field of biomarkers. Due to their special photophysical properties, quantum dots are increasingly widely used as fluorescent probes and play an increasingly important role in the field of biomarkers. [0003] Quantum dots are semiconductor crystals with nanometer dimensions. Compared with traditional fluorescent dyes, quantum dots possess many unique optical properties, such as broad absorption spectrum, narrow and symmetrical emission spectrum, resistance to bleaching, high brightness and long fluorescence lifetime, etc. Due to their e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/88C09K11/02C12Q1/02
Inventor 石勇铨刘浩琪崔大祥汤玮
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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