44 results about "Influenza virus Antibody" patented technology

Provided is an anti-influenza virus antibody that exhibits neutralizing activity beyond the barrier of the two groups of influenza viruses categorized according to the conservativeness of hemagglutinin amino acids, a method of producing the same, and a test method for determining whether the subject carries the neutralizing antibody.

The invention discloses an H1N1 influenza virus antibody and application thereof in ultra-micro detection of H1N1 viruses. The H1N1 influenza virus antibody comprises a heavy chain variable region, afirst heavy chain hypervariable region, a second heavy chain hypervariable region, a third heavy chain hypervariable region, a light chain variable region, a first light chain hypervariable region, asecond light chain hypervariable region and a third light chain hypervariable region. The H1N1 influenza virus antibody can be specifically combined with the H1N1 influenza viruses, and is used for ultra-micro detection of H1N1 influenza to achieve early prevention and treatment of the H1N1 influenza, thereby being of great economic and social significance.

The present invention provides structural determinants important for binding to the stem domain of the HA protein of influenza virus, and methods of use thereof for production of high affinity neutralizing influenza virus antibodies based upon these determinants. The present invention further provides tools for determining the efficacy of an influenza virus vaccine. The present invention further provides a molecular signature useful for determining the efficacy of an influenza virus vaccine in a subject, or for predicting prior immunologic exposure or antigen responsiveness to vaccine or influenza virus infection.

The invention relates to the biotechnology field, in particular to an influenza virus antibody. A light-chain variable region of the influenza virus antibody has an amino acid sequence shown as the SEQ ID No.1; a heavy-chain variable region of the influenza virus antibody has an amino acid sequence shown as the SEQ ID No.2. The antibody can well neutralize H3N2, H4N6 and H14N5 subtype influenza viruses, combine HA proteins of all subtype influenza viruses in a group 2, neutralize H3-subclade viruses and inhibit copying of the H3 subtype influenza viruses in a mouse body and has the important economic and social significance.

The invention first provides a preparation method of a swine H1N1 subtype influenza virus hemagglutinin (HA) recombinant protein. A pair of primers with HIS labels is designed by removing a signal peptide of the HA according to an HA sequence, and a prokaryotic expression plasmid with a 6*His-maltose-binding protein (MBP) combination label is constructed; the HA recombinant protein expressed in a soluble form is successfully obtained in an Escherichia coli expression system and can generate reaction with a mouse antibody with swine H1N1 subtype influenza virus HA, thereby showing that the HA recombinant protein has good antigenicity; a liquid phase chip detection technology is established by using the HA recombinant protein and is higher than ELISA (Enzyme-Linked Immuno Sorbent Assay) in sensitivity; the establishment of the method provides necessary technical supplement for detecting a swine influenza virus antibody, lays a technical basis for researching the liquid phase chip detection technologies of other epidemic disease antibodies, and provides a test reference for establishing multiple liquid phase chip detection technologies of multiple swine disease antibodies.

This invention disclose a kind of equipment used to detect the fowl influenza virus antibody correctly, fast, sensitively, with high specificity and economically. The equipment includes immunity sensor, enzyme labeled double antibody reaction system, electrolytic cell and data collection and process system; the immunity sensor includes the electrode system which is composed with the work electrode, the reference electrode and the assistant electrode which are printed in the insulated base plate; the electrolytic cell includes the detection base liquid, the beater and the temperature controller; the data collection and process system includes the electrochemistry workstation, the computer and the software system; the immunity sensor connects the electrochemistry workstation by lead; the work electrode of the immunity sensor is carbon electrode, which has coated the gold size induction film embed fowl influenza virus antibody. This invention also discloses a kind of method to use the equipment to detect the fowl influenza virus antibody.

The invention provides a kind of influenza A virus IgA antibody immunofluorescence detection test strip and a preparation method thereof, wherein the influenza A virus IgA antibody immunofluorescence detection test strip comprises a sample pad, a marker pad, a nitrocellulose membrane, Absorbent paper and backboard; the nitrocellulose membrane includes the detection line of the influenza A virus NP protein antigen polypeptide fragment and the human IgA antibody control line; the marker pad is provided with an anti-human IgA antibody-fluorescent microsphere marker. The detection test paper provided by the invention has extremely high detection sensitivity, and the detection result can be obtained with the naked eye without resorting to an expensive PCR detector, thereby realizing rapid screening of influenza A virus. The invention also provides a detection method and application of the above-mentioned influenza A virus IgA antibody immunofluorescence detection test strip. The detection method has simple steps, easy operation, rapid results and is suitable for wide promotion.

The present invention provides a kind of influenza B virus IgA antibody immunofluorescence detection test strip and its preparation method, said type B influenza virus IgA antibody immune immunofluorescence detection test strip comprises a sample pad, a marker pad, a nitrocellulose membrane, Absorbent paper and backboard; the nitrocellulose membrane includes the detection line of the influenza B virus NP protein antigen polypeptide fragment and the human IgA antibody control line; the marker pad is provided with an anti-human IgA antibody-fluorescent microsphere marker. The detection test paper provided by the invention has extremely high detection sensitivity, and the detection result can be obtained with the naked eye without resorting to an expensive PCR detector, thereby realizing rapid screening of influenza B virus. The invention also provides a detection method and application of the above-mentioned influenza B virus IgA antibody immunofluorescence detection test strip. The detection method has simple steps, easy operation, rapid results and is suitable for wide promotion.