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68 results about "Strain specificity" patented technology

Fusarium oxysporum bitter gourd specialized molecular marker and application thereof

The invention discloses a fusarium oxysporum bitter gourd specialized RAPD (random amplified polymorphic DNA) marker (SEQ.ID.No.1), an SCAR (sequence characterized amplified region) marker (SEQ.ID.No.2) obtained by transforming the RAPD marker and application of the molecular markers in pathogenic bacteria identification and pathogenetic tissue detection. The molecular marker disclosed by the invention has fusarium oxysporum bitter gourd specialized strain specificity, consistency and stability, can distinguish difference between the specialized strain and other specialized pathogenic bacteria, can identify fusarium oxysporum bitter gourd specialized pathogens and plays an important role in identification of the fusarium oxysporum bitter gourd specialized strain, so that a foundation is laid for building a special, quick, high-sensitivity, stable and reliable molecular detection technical system for fusarium oxysporum bitter gourd specialized pathogenic bacteria. By applying the molecular marker disclosed by the invention, the defects that workload is high and cycle is long in the conventional fusarium oxysporum bitter gourd specialized pathogenic bacterium identifying and distinguishing process can be overcome, and quick and accurate identification and detection on pathogens are realized.
Owner:GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI

Plasmid reference molecule of quantitative determination of nucleic acids from transgenic soybean GTS40-3-2

The invention discloses a plasmid reference molecule which is suitable for the quantitative determination of nucleic acids from a transgenic soybean GTS40-3-2, comprising a structure-specific sequence and a strain-specific sequence of the transgenic soybean GTS40-3-2 and a specific fragment of a soybean endogenous reference gene Lectin. The invention is characterized by designing primers to obtain the structure-specific sequence and the strain-specific sequence of the transgenic soybean GTS40-3-2 and the specific fragment of the soybean endogenous reference gene Lectin by PCR amplification through the analysis of inserting the exogenous source of the transgenic soybean GTS40-3-2 in the gene sequence; constructing by molecular cloning to form a artificial recombinant plasmid molecule pXL02 in a plasmid molecule; detecting the phosphorus content of the plasmid reference molecule by inductively coupled plasma mass spectrometry (ICP-MS) to calculate the concentration of the plasmid reference molecule. According to the invention, the plasmid reference molecule constructed in the invention can completely replace a transgenic soybean GTS40-3-2 positive standard sample, and the plasmid reference molecule is completely suitable for the quantitative PCR analysis and detection of structure specificity and strain specificity of the transgenic soybean GTS40-3-2 sample.
Owner:SHANGHAI INST OF MEASUREMENT & TESTING TECH

SSR marker fingerprint spectrum of needle mushroom Qinghe strain and application thereof

The invention relates to an SSR marker fingerprint spectrum of a flammulina velutipes Qinghe strain and application thereof. The SSR marker fingerprint spectrum of the flammulina velutipes Qinghe strain is combined by six pairs of peculiar allelic fragments, developed on the basis of a flammulina velutipes genomic sequence and marked with SSR. Application of the SSR marker fingerprint spectrum of the flammulina velutipes Qinghe strain comprises that SSR marker amplification is carried out on a flammulina velutipes strain, the obtained banding pattern is compared with the fingerprint spectrum, and if the banding pattern is consistent with the fingerprint spectrum, the flammulina velutipes strain is the flammulina velutipes Qinghe strain. Compared with the conventional morphological detection, the conventional antagonistic test and the conventional fruiting test, the SSR marker fingerprint spectrum of the flammulina velutipes Qinghe strain has the advantages of short detection time, high accuracy and good repeatability and has Qinghe strain specificity in 24 collected major flammulina velutipes strains in China.
Owner:SHANGHAI ACAD OF AGRI SCI

Strain specificity PCR detection method of heredity modified corn strain MON863

The invention relates to strain specificity PCR testing method of improved corn strain MON863 that includes 5'end strain specificity testing method and 3'end strain specificity testing method. The process includes: analyzing external inserting carrier neighbor gene sequence of MON863 corn, designing primer of strain specificity PCR and ration PCR, and probe; building and optimizing strain specificity and ration PCR system; verifying the strain specificity and ration PCR testing method to build the testing platform of strain specificity PCR of improved corn strain MON863.
Owner:SHANGHAI JIAO TONG UNIV

Standard plasmid molecule used for detecting genetically modified soybeans and cotton and building method thereof

The invention discloses a standard plasmid molecule used for detecting genetically modified soybeans and cotton and a building method thereof. The standard plasmid molecule contains eight gene specific fragments (Lec1, 35S, NOS, PAT, Sad1, Cry1Ab / c and EPSPS) of soybeans and cotton. The standard plasmid molecule and the building method have the following beneficial effects: the eight genes are fused into a large fragment through fusion PCR (polymerase chain reaction) and the large fragment is cloned on a plasmid vector to obtain a recombinant plasmid molecule pTLE8; the standard plasmid molecule built in the invention can replace the positive controls and is suitable for the screening of the genetically modified soybean strain GTS40-3-2 and Bt cotton and the qualitative and quantitative PCR analysis and detection of the gene and strain specificity; and new exogenous gene fragments can be added on the basis of the original standard molecule if new genetically modified materials occur, thus meeting the increasing detection requirements of the genetically modified crop materials.
Owner:FEED RESEARCH INSTITUTE CHINESE ACADEMY OF AGRICULTURAL SCIENCES

Novel epitope and mechanism of antigen-antibody interaction in an influenza virus

Antibodies (Abs) play roles in protection against influenza. Neutralizing Abs either inhibit the binding of hemagglutinin (HA) to cellular receptors or prevent the conformational change of HA induced by low pH. The former Ab binds to the regions near the sialic acid-binding pocket on the globular head formed by HA1 and generally shows narrow strain specificity. The latter Ab binds to the stem region formed mainly by HA2 and shows broad strain specificity. We isolated a broadly neutralizing Ab against H3N2 viruses. X-ray analysis of the HA / Ab complex indicated that the Ab binds to the valley formed by two neighboring HA monomers at the side of the globular head. The Ab shows neutralizing activity by preventing the conformational change of HA induced at low pH.
Owner:FUJITA HEALTH UNIVERSITY

Biocidal molecules, macromolecular targets and methods of production and use

InactiveUS20060240494A1Restrains essential movementInhibit activity of proteinCompound screeningBiocideScreening methodDirect binding
A method for identifying a compound that has a biocidal effect against a selected organism involves screening from among known or unknown peptide or non-peptide molecules, a test molecule that binds selectively to a target sequence of a multi-helical lid of a heat shock protein of the organism. The binding of the test compound inhibits the protein folding activity of the protein. A specific embodiment of such a method is useful for identifying or designing a pharmaceutical or veterinary biocidal or antibiotic compound, preferably a pathogen and / or strain-specific compound. For this purpose, the compound does not bind to a heat shock protein that is homologous to the mammalian subject to be treated with the compound. Screening methods can encompass direct binding or competitive assays. Molecules or compounds identified by these methods are employed as biocides for pharmaceutical, veterinary, pesticide, insecticide and rodenticide uses, among others.
Owner:THE WISTAR INST OF ANATOMY & BIOLOGY +1
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