47 results about "Direct binding" patented technology

A method for identifying compounds binding to HCV polymerase comprising the steps of: contacting said HCV polymerase or an analog thereof with a probe formula I:

wherein A is O, S, N, NR¹, or CR¹, wherein R¹ is defined herein;

• ----- represents either a single or a double bond;
• R² is selected from: H, halogen, R²¹, OR²¹, SR²¹, COOR²¹, SO₂N(R²²)₂, N(R²²)₂, CON(R²²)₂, NR²²C(O)R²² or NR²²C(O)NR²² wherein R²¹ and each R²² is defined herein;
• B is NR³ or CR³, wherein R³ is defined herein;
• with the proviso that, when A is not N, then one of A or B is either CR¹ or CR³,
• K is N or CR⁴, wherein R⁴ is defined herein;
• L is N or CR⁵, wherein R⁵ has the same definition as R⁴ defined above;
• M is N or CR⁷, wherein R⁷ has the same definition as R⁴ defined above;
• R⁵ is C(Y¹)Z wherein Y¹ is O or S; and
• Z is N(R^6a)R⁶ or OR⁶, wherein R^6a is H or alkyl or NR⁶¹R⁶² wherein R⁶¹ and R⁶² are defined herein; and R⁶ is H, alkyl, cycloalkyl, alkenyl, Het, alkyl-aryl, alkyl-Het; or R⁶ is

wherein R⁷ and R⁸ and Q are as defined herein;

• Y² is O or S;
• R⁹ is H, (C_1-6 alkyl), (C_3-7)cycloalkyl or (C_1-6)alkyl-(C_3-7)cycloalkyl, aryl, Het, (C_1-6)alkyl-aryl or (C_1-6)alkyl-Het, all of which optionally substituted with R⁹⁰; or R⁹ is covalently bonded to either of R⁷ or R⁸ to form a 5- or 6-membered heterocycle; or a salt thereof; where the probe comprises a detectable label attached to any suitable position, whereby said probe binds to an HCV polymerase or an analog thereof and is capable of being displaced by an inhibitor thereof.

The invention provides methods for the detection, quantification, identification and structural analysis of one or more molecules. Mass spectrometry (MS) is not a universal detector as all molecules do not ionize equally well leading to poor signal to quantity information. MS can be optimized to identify the specific mass of a binding component when the presence of a material is known. Colorimetric resonant reflectance optical sensors provide a universal mass detector in that nearly all biological masses give equally proportional signals. The combined methods allow selection and or detection with quantification of all masses binding to the sensor with the ability to identify specific molecules by their individual masses and structure analyses.

The invention relates to a porous concrete ecological capsule slop protection process, which comprises making ecological capsules which are braided by fiber material such as palm ropes and twines and the like, storing doras, water-retention material, slow-release fertilizer, insecticide and plant seeds, placing the ecological capsules in the grooves of the porous concrete ecological capsule blocks, matching an upper and a lower porous concrete ecological capsule blocks through the way of bolt or direct binding, placing the porous concrete ecological capsule blocks on hard slope protection, connecting each porous concrete ecological capsule block with bolts or jump rings, and binding the space between the porous concrete ecological capsule blocks with cement and wholly hinging on the top portion of the slop protection. The advantages are that solving the difficulty of the ecological repair of the hard slop protection, guaranteeing the normal growth of plants which are covered on the hard slop protection, and purifying water and increasing water landscape. The process is applied on the hard slop protection project of river and lakes, which solves the problems of plant growing stroma loss which is caused by scouring, plant growing difficulty and reducing flood control function which is caused by the improper reconstruction for the original slop protection.

The invention discloses a super-hydrophobic coating capable of being used underwater and a preparation and application method thereof, and belongs to the technical field of super-hydrophobic coatings. The super-hydrophobic coating is formed by a backing material and a hydrophobic material. The backing material is prepared from, by mass, 5% to 30% of sealing rubber, 5% to 30% of petroleum resin, 1% to 10% of flatting agent and 30% to 89% of organic solvent A. The backing material and the hydrophobic material are used in combination, and on the basis of guaranteeing super-hydrophobic performance, the backing material is utilized for increasing the direct binding strength of a super-hydrophobic coating and a base material; damage of water flow/steam and the like to the super-hydrophobic coating can be effectively reduced, use strength is increased, the super-hydrophobic state can be maintained in the underwater/high-humidity environment for a long time, the service life can be prolonged, and great significance is achieved on oceangoing voyage, underwater exploration and the like.

The invention relates to the fields of tumour physiology and biotechnology. The object of the invention is to develop effective and selective novel fusion proteins and fusion protein-antibody complexes against various types of leukaemia and solid tumours. Selectivity is achieved by cell-specific or tumour-specific ligands of the fusion proteins or by antibodies of the fusion protein-antibody complexes. Effectiveness is achieved on the one hand by the direct binding of the microtubules or cytoskeleton elements to the microtubule-binding regions and on the other hand by induction, as well as by the reinforcement of the immune reaction by regions that trigger the immune reaction on the target cells.

The present invention relates to a multi-conjugate of small interfering RNA (siRNA) and a preparing method of the same, more precisely a multi-conjugate of siRNA prepared by direct binding of double stranded sense/antisense siRNA monomers or indirect covalent bonding mediated by a cross-linking agent or a polymer, and a preparing method of the same. The preparing method of a siRNA multi-conjugate of the present invention is characterized by simple and efficient reaction and thereby the prepared siRNA multi-conjugate of the present invention has high molecular weight multiple times the conventional siRNA, so that it has high negative charge density, suggesting that it has excellent ionic interaction with a cationic gene carrier and high gene delivery efficiency.

The invention discloses a method for identifying a same user. The method comprises the following steps: establishing a direct binding relationship between every two articles which are used in a combined manner; and identifying a user of all the articles which have the direct binding relationships with the same article as the same user, or identifying a user of all the articles which have the direct binding relationships and indirect binding relationships with the same article as the same user. The method provided by the embodiment of the invention has the advantage that the article user can bemore accurately identified.

The present disclosure relates to compositions and methods of modulating inflammatory and immune responses through binding of SAA to TLR2 in a subject (e.g., human, non-human primate, rodent, etc.), and compositions and methods for screening TLR2 agonists and antagonists. In the studies described herein, a potential role of SAA in neutrophilia was investigated and the results demonstrated that SAA is a potent inducer for macrophage secretion of G-CSF, which leads to neutrophilia in mice. Using G-CSF^−/− and TLR2^−/− mice, it was found that the SAA-induced neutrophilia is dependent on TLR2-mediated production of G-CSF. Based on direct binding assay and gain-of-function studies in TLR2-transfected cells, SAA was identified as a novel ligand for TLR2 and a link between increased SAA concentration and TLR2-mediated inflammatory responses such as neutrophilia was established. Additional embodiments are disclosed.

The invention discloses a local device binding method. The method comprises the following steps: allocating an access address to a control device; enabling the control device to send control information containing the identification information of the access address; enabling the access address to receive the control information; enabling a controlled device to subscribe the access address; and the controlled device to acquire the control information from the access address. Compared with the prior art, the invention has the following advantages that the relates to the local binding and control technology based on the BLE mesh technology; when a remote controller or a sensor and intelligent equipment such as a lamp or a socket are in the same network, the directional control over the intelligent equipment can be achieved through direct binding of the remote controller or the sensor and the intelligent equipment, an APP can check and modify the binding relation, and a more flexible andsafer control mode is achieved; moreover, a flexible linkage control function between the sensor and the intelligent equipment is realized, the necessity of the gateway is reduced, and the cost is reduced.

The invention discloses an air valve surface strengthening treatment method. The binding force between an air valve and a nitride layer and between the nitride layer and a chromium cladding layer is reinforced by first performing overall nitriding on the air valve, then performing chromium electroplating on a rod part of the air valve and finally performing diffusion annealing. According to the air valve surface strengthening treatment method, micro-cracks produced by nitriding are made up by filling the chromium cladding layer, and then Cr and N compounds are generated on the surface of the air valve through an annealing method, so that mechanical binding of the Cr and N in the micro-cracks is changed into chemical binding; therefore, the influence of the micro-cracks of the nitride layeris weakened; and the defect that the binding force between the chromium cladding layer and an air valve substrate is not strong because of direct binding in the conventional chromium electroplating method is effectively overcome.