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Device for detecting avian influenza virus antibody and detection method

An avian influenza virus and antibody technology, applied in measurement devices, instruments, material analysis by electromagnetic means, etc., can solve the problems of long detection time, low sensitivity, and prone to false positives, and achieve high specificity and low price. Effect

Inactive Publication Date: 2012-07-18
ZHEJIANG GONGSHANG UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Hemagglutination and hemagglutination inhibition tests are cumbersome and prone to false positives; the agar immunodiffusion test method is convenient to obtain materials and easy to operate, but the detection time is long and the sensitivity is low; immunofluorescence technology and enzyme-linked immunosorbent assay require special instruments Equipment, the detection process is complicated, the cost is high, the detection time is long, and false positives are prone to occur

Method used

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  • Device for detecting avian influenza virus antibody and detection method
  • Device for detecting avian influenza virus antibody and detection method

Examples

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Effect test

Embodiment 1

[0034] Example 1: The device for detecting avian influenza virus antibodies of the present invention is composed of an immunosensor, an enzyme-labeled secondary antibody reaction system, an electrolytic cell, and a data acquisition and processing system.

[0035] The immunosensor includes a working electrode 2, a reference electrode 4 and an auxiliary electrode 3, each electrode is printed on the rubber resin plate 1 by screen printing, the auxiliary electrode 3 is a graphite electrode, the reference electrode 4 is an Ag / AgCl electrode, and the working electrode 2. The carbon electrode is used as the supporting electrode, and the chitosan gold film that embeds the H5N1 avian influenza virus antigen is coated on it;

[0036] The enzyme-labeled secondary antibody reaction system is a dilution of rabbit anti-chicken IgG antibody labeled with horseradish peroxidase;

[0037] The electrolytic cell includes a detection bottom liquid, a stirrer and a thermostat. The detection bottom...

Embodiment 2

[0045] Example 2: Method for detecting avian influenza virus antibodies

[0046] 1) Sample collection and processing

[0047]The poultry blood was taken, centrifuged to obtain serum, which was diluted for later use.

[0048] 2) Drop the sample to be tested on the surface of the working electrode 2 of the immunosensor of the present invention, and incubate at 20° C. for 10 minutes, so that the avian influenza virus antibody in the sample and the virus antigen attached to the surface of the colloidal gold particle on the working electrode 2 of the immunosensor are produced. Antigen-antibody specific reaction. Wash the immunosensor three times with PBS-T and then with double distilled water;

[0049] 3) Put the immunosensor into the electrolytic cell, and the detection bottom liquid of the electrolytic cell contains 0.4 mmol / L H 2 O 2 1mmol / L thionine acetate buffer, pH value is 7.0.

[0050] 4) Set the scan rate to 0.1V / s, apply a voltage to the working electrode, and measu...

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Abstract

This invention disclose a kind of equipment used to detect the fowl influenza virus antibody correctly, fast, sensitively, with high specificity and economically. The equipment includes immunity sensor, enzyme labeled double antibody reaction system, electrolytic cell and data collection and process system; the immunity sensor includes the electrode system which is composed with the work electrode, the reference electrode and the assistant electrode which are printed in the insulated base plate; the electrolytic cell includes the detection base liquid, the beater and the temperature controller; the data collection and process system includes the electrochemistry workstation, the computer and the software system; the immunity sensor connects the electrochemistry workstation by lead; the work electrode of the immunity sensor is carbon electrode, which has coated the gold size induction film embed fowl influenza virus antibody. This invention also discloses a kind of method to use the equipment to detect the fowl influenza virus antibody.

Description

technical field [0001] The invention relates to the technical field of rapid detection of avian influenza virus antibodies, in particular to an electrochemical sensor and a detection method for detecting avian influenza virus antibodies based on antigen-antibody reaction. Background technique [0002] Avian Influenza virus (AIV) is a highly infectious virus originating from poultry, which can cause the death of a large number of livestock and human beings in a very short period of time. In recent years, highly pathogenic avian influenza broke out in many countries and regions, which not only caused huge economic losses to related industries, but also seriously threatened human health and safety. [0003] Rapid detection of avian influenza virus antibodies is one of the more commonly used methods in avian influenza epidemic detection, disease diagnosis, pre-slaughter quarantine, and immune effect detection. [0004] At present, the detection of avian influenza virus antibodi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/569G01N33/558G01N27/416
Inventor 赵广英吴淑春
Owner ZHEJIANG GONGSHANG UNIVERSITY
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