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Preparation method of near-ultraviolet polystyrene copolymerization fluorescence microsphere

A fluorescent microsphere, polystyrene technology, applied in chemical instruments and methods, luminescent materials, etc., can solve the problems of destroying polymerization conditions, difficult to copolymerize fluorescent microspheres with polystyrene, etc., and achieve high luminous efficiency and good monodispersity. , the effect of uniform particle size

Inactive Publication Date: 2014-04-23
ZHONGBEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] However, most of the fluorescent dyes used in the existing reports are fluorescent materials that emit red or green light, and no fluorescent materials that emit blue light are used.
In addition, according to reports in the literature (Journal of Materials Chemistry, 2009, 19, 2018-2025), due to the large size of fluorescent dye molecules with double bonds, there is a certain degree of steric hindrance during the copolymerization process, which has a self-inhibiting polymerization effect, and at the same time The added double-bond fluorescent dye will destroy the stable polymerization conditions of the original blank microspheres, and it is difficult to form polystyrene copolymerized fluorescent microspheres with uniform particle size

Method used

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  • Preparation method of near-ultraviolet polystyrene copolymerization fluorescence microsphere
  • Preparation method of near-ultraviolet polystyrene copolymerization fluorescence microsphere
  • Preparation method of near-ultraviolet polystyrene copolymerization fluorescence microsphere

Examples

Experimental program
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Embodiment 1

[0025] Take 0.024 g of azobisisobutyronitrile, 0.072 g of polyvinylpyrrolidone, and 9.0 g of absolute ethanol in a microreactor, and ultrasonically obtain a clear and transparent solution. Add 1.2 g of styrene and 7.2 mg of N-allyl carbazole, pass nitrogen to remove oxygen, and seal. Placed in a constant temperature oscillator, reacted at 70°C, 130rpm for 12h. The obtained microspheres were repeatedly washed with water and ethanol until the fluorescence was no longer detected in the washing solution, and then vacuum-dried at 50° C. to obtain polystyrene copolymerized fluorescent microspheres.

[0026] Weigh 1.0 g of the above polystyrene copolymerized fluorescent microspheres, add 28 mL of concentrated sulfuric acid, ultrasonically disperse the fluorescent microspheres in concentrated sulfuric acid, react at 55°C for 7 hours, wash the sample with water until neutral, and dry it in vacuum at 50°C to obtain Sulfonic acid-based functional near-ultraviolet polystyrene copolymeriz...

Embodiment 2

[0028] Take 0.028 g of azobisisobutyronitrile, 0.067 g of polyvinylpyrrolidone, and 9.8 g of absolute ethanol in a microreactor, and ultrasonically obtain a clear and transparent solution. Add 1.4 g of styrene and 11.6 mg of N-allylcarbazole, pass nitrogen to remove oxygen, and seal. Placed in a constant temperature oscillator, reacted at 70°C, 130rpm for 12h. The obtained microspheres were repeatedly washed with water and ethanol until the fluorescence was no longer detected in the washing solution, and then vacuum-dried at 50° C. to obtain polystyrene copolymerized fluorescent microspheres.

[0029] Weigh 0.064g AIBN and disperse in 25.6mL ethanol / deionized water (5:1) mixed solvent. Weigh 0.2 g of the above-mentioned polystyrene copolymerized fluorescent microspheres and add them into the mixed solvent, and ultrasonically disperse them. Nitrogen was passed to remove oxygen, and 0.832 g of γ-methacryloxypropyltrimethoxysilane was added at the same time, sealed and shaken e...

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Abstract

The invention discloses a preparation method of a near-ultraviolet polystyrene copolymerization fluorescence microsphere, and the preparation method is as follows: putting initiator azodiisobutyronitrile and dispersing agent polyvinylpyrrolidone in absolute ethanol to obtain a clear and transparent solution, simultaneously adding styrene and N-allyl carbazole to the solution for copolymerization reaction under the protection of nitrogen to obtain a polystyrene copolymerization fluorescence microsphere, and then introducing sulfonic acid groups or hydroxyl groups onto the surface of the polystyrene copolymerization fluorescence microsphere to produce the functional near-ultraviolet polystyrene copolymerization fluorescence microsphere. The preparation method adopts a method of dispersion polymerization to prepare the near-ultraviolet polystyrene copolymerization fluorescence microsphere having good monodispersity and stable fluorescent properties.

Description

technical field [0001] The invention relates to a method for preparing fluorescent microspheres, in particular to a method for preparing near-ultraviolet functional polystyrene copolymerized fluorescent microspheres. Background technique [0002] Fluorescent microspheres have a stable morphological structure and high luminous efficiency, and are less affected by external conditions such as solvents, heat, electricity, magnetism, etc. than pure fluorescent compounds, and have extremely important applications in many fields, especially in the biomedical field, such as Immunological detection, cell labeling and high-throughput drug screening, immobilized enzymes, detection of cell surface antigens, degenerative neuropathy tracers, detection of phagocytosis, genetic research, quality control of sensitive diagnostic reagents, etc. Among them, monodisperse micron-scale fluorescent microspheres (1-10 μm) are widely used, especially in flow cytometry. [0003] CN 1278534A discloses...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08F212/08C08F226/06C08F8/36C08F257/02C08F230/08C09K11/06
Inventor 刘清浩郭金春刘红彦王俊美
Owner ZHONGBEI UNIV
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