46results about How to "Good potential for clinical application" patented technology

The present invention relates to the removal of bilirubin from blood, and is especially cyclodextrin crossed polymer microsphere for adsorbing bilirubin and its preparation and application. The microsphere is prepared with diisocyanate as crosslinking agent and through condensation with cyclodextrin monomer. The polymer microsphere of the present invention has low cost and results in simplified experiment process, and the efficient removal of bilirubin with the polymer microsphere shows that the said material has excellent clinical application latent.

The invention discloses a method for preparing a supermolecule capsule with multiple drug release stimulation and MRI radiography ability. A ferroferric oxide microcapsule is prepared as a novel multifunctional magnetic resonance noise and drug therapy medicament through a layer-by-layer self-assembly technology. Through the host-guest interaction and the Coulomb interaction of different supramolecular polymers, the medicine molecule is controllably released through the non-invasive ultraviolet radiation distributed azobenzene isomerism and the acid response Schiff base. Additionally the magnetic-targeted MRI contrast agent is obtained by encapsulating the superparamagnetic ferroferric oxide granule. The r2 relaxation rate of the microcapsule is increased by 37% to reach 125.71 mM-1S-1 compared with that of the neutral condition (92.02mM-1S-1) through the stimulation of subacidity (pH=5.6).

The invention discloses preparation of dendritic molecule-modifiedhydrophilic immunomagnetic beads and application of the hydrophilic immunomagnetic beads to rapid and efficient cell capture. The preparation method comprises the following steps: firstly, synthesizing magnetic nanoparticles through a hydrothermal method; hydrolyzing ethyl orthosilicate and modifying with a silylating reagent in order that the surface of a material is rich in amino groups; coupling dendritic molecules to the surface of the material in order that the material is hydrophilic; lastly, fixing an antibody to the surface of the material to prepare a capture substrate capable of recognizing tumor cells specifically. As proved by experiments, the hydrophilic immunomagnetic beads modified by the antibody and the dendritic molecules can shorten the time of combining the material and cells, perform high-specificity recognition and capture tumor cells rapidly, and capturing efficiency of 86+ / -5 percent can be achieved only in 15 minutes; meanwhile, the activity of the cells is kept, and the tumor cells can be successfully separated from whole blood into which a tumor cell standard is added. A template-free, low-cost and low-toxicity material is applied to cell capture, so that the method is novel, convenient, practical and efficient, and has a tremendous clinical application potential.

The invention discloses a phenol recognition SERS probe, a preparation and an application thereof, and a universal ultra-sensitive immunoassay method based on SERS. Firstly, a phenol responsive SERS probe is prepared by reducing chloroauric acid with DTDBA, ahen, ELISA is combined with SERS, biomolecules are labeled with ALP, a substrate PPNA is hydrolyzed by ALP to generate phenol. An SERS probesignal caused by the phenol is used for realizing a technology for sensitive detection of the biomolecules. The technology can not only overcome the disadvantages of low sensitivity of the conventional enzyme linked immunosorbent assay, but also can solve the problems of enhancement and poor reproducibility of SERS detection signals. The phenol recognition SERS probe disclosed by the invention hasremarkable universality, can be widely used for immunoassay using the ALP as an enzyme label to measure a variety of biomolecules, lays a solid foundation for the development of immune technology based on SERS detection, and has a very large development space and a broad application prospect in the fields of biological and chemical detection, medical diagnosis, and the like.

The invention provides a biological 3D printing ink and a preparation method thereof. The preparation method comprises the following steps: S1: preparing a calcium hydroxide saturated solution, addinga silica nanoparticle suspension to the calcium hydroxide saturated solution, centrifuging and taking a precipitate to obtain modified nano-bioglass particles; S2: dissolving gelatin and sodium alginate in deionized water, then adding the modified nano-bioglass particles prepared in the Step S1, stirring evenly to prepare liquid hydrogel, sterilizing and equilibrating to obtain a gelatin-sodium alginate composite hydrogel; S3: dissolving solid calcium chloride powder in deionized water to prepare a calcium chloride solution, and sterilizing to obtain a cross-linking agent; and S4: adding thecross-linking agent to the gelatin-sodium alginate composite hydrogel for cross-linking to obtain the biological 3D printing ink. The biological 3D printing ink prepared by the invention has not onlysignificantly improved mechanical properties but also good gelling performance, rich pores and good cell-carrying potential.

The present invention relates to pleuromutilin antibiotic derivatives represented by a general formula (I), pharmaceutically acceptable salts, prodrugs, solvates or stereoisomers thereof, wherein R<2>, Q<1>, Q<2>, m and X are defined in an instruction. The present invention further relates to preparation methods of the compounds, drug compositions containing the compounds, drug preparations containing the compounds, and applications of the compounds in preparation of drugs for treatment and / or prevention of diseases caused by microorganisms.

The invention relates to the field of biological medicine, in particular to fusion protein of a cytokine combined chimeric antigen receptor and an application of the fusion protein. The fusion protein comprises a chimeric antigen receptor, 2A peptide, IL-7, 2A peptide and CCL3 which are sequentially connected in series, the chimeric antigen receptor comprises an scFv region, a hinge region, a transmembrane domain and an intracellular signal transduction region. According to the fusion protein of the cytokine combined chimeric antigen receptor and the application of the fusion protein, two cytokines IL-7 and CCL-3 are added during construction of a CAR vector, and the two cytokines can enhance the anti-apoptosis effect of CAR T cells and reduce the immunosuppression effect of the CAR T cells, so that the CAR T cells are helped to enhance the anti-tumor effect.

The invention belongs to the technical field of magnetic resonance imaging (MRI) contrast agents, and discloses a preparation method of a sericin-gadolinium pH responsive targeting tumor nuclear magnetic resonance contrast agent. The contrast agent is used for enhancing MRI research of tumor tissues, sericin, gadolinium acetate hexahydrate and gadolinium chloride hexahydrate are adopted as raw materials, and the nano contrast agent SS@GAH-GdCl3 is synthesized through a Schiff base reaction. Amino groups of the sericin and aldehyde groups of the gadolinium acetate hexahydrate are subjected to aone-step reaction by a two-step method to form Schiff base, and gadolinium ions are supplemented through electrostatic adsorption of the gadolinium chloride hexahydrate. The contrast agent prepared by the method can smoothly pass through normal tissues and blood vessels, and the surface potential of the contrast agent can be automatically reversed at a tumor part to enter tumor tissues, so that the uptake of tumor cells is increased, and the precise MRI contrast of solid tumors is realized; and the metabolism time of the nano contrast agent is remarkably prolonged by 30-60 min, and is far longer than the pharmacokinetic time of a commercial gadodiamide injection.

The invention belongs to the technical field of biomacromolecular separation analysis and specifically relates to a surface enhanced raman scattering substrate for a gold nucleus 'cell raman silent zone', a preparation method and an application thereof in cell raman imaging. The method comprises the following steps: firstly, compounding 50-60nm gold nanoparticles according to a hydrothermal method and dissolving in a buffer solution; adding 'raman silent zone' molecule (E)-2-((4-(phenyl ethynyl) benzal) amino) ethanethiol and slightly agglomerating in the solution; adding dopamine into the buffer solution and connecting a polypeptide sequence with the golden ball surface through a quinonyl group generated by the auto polymerization of dopamine, thereby obtaining the surface enhanced raman scattering substrate for cell nucleus imaging of tumor cells. The material applied to the cell raman imaging has the advantages of high speed, high efficiency, simple operation and huge clinical application potentiality.

The present invention relates to the removal of bilirubin from blood, and is especially cyclodextrin crossed polymer microsphere for adsorbing bilirubin and its preparation and application. The microsphere is prepared with diisocyanate as crosslinking agent and through condensation with cyclodextrin monomer. The polymer microsphere of the present invention has low cost and results in simplified experiment process, and the efficient removal of bilirubin with the polymer microsphere shows that the said material has excellent clinical application latent.

The invention discloses a metal organic framework composite nanomaterial as well as a preparation method and application thereof. The metal organic framework composite nano material comprises a functional material and a copper metal organic framework carrying the functional material, wherein the functional material comprises small organic molecules. The metal organic framework composite nanomaterial is good in chemical stability, has tumor microenvironment responsiveness, and can realize non-invasive, in-situ and efficient treatment and real-time monitoring of tumor parts.

The invention discloses an artificially synthesized anticancer peptide and an acid responsive nanoparticle precursor thereof. The nanoparticle is formed by connecting an anti-cancer peptide and an amphiphilic monomethyl polyethylene glycol-polypropylene glycol polymer through an acid-responsive chemical bond. Tests prove that the anti-cancer peptide can kill tumor cells through membrane rupture activity, and has the advantages of excellent broad-spectrum anti-cancer activity and drug resistance. The nanoparticles are selectively sensitive to a tumor subacid environment, can completely release anti-cancer peptides, and play an active role of a targeted tumor cell line. And the polypeptide has the advantages of hemolysis resistance, high plasma stability, low in-vivo systemic toxicity, systemic drug delivery and the like. The nanoparticle has a remarkable inhibition effect on various tumor cells including triple negative breast cancer, and particularly has a relatively good clinical application potential on drug-resistant triple negative breast cancer.

The invention discloses an MMT (montmorillonite)-Gd-DTPA (diethylenetriaminepentaacetic acid) compound, a synthetic method thereof and an application of the MMT-Gd-DTPA compound to magnetic resonance diagnosis for a digestive tract. The synthetic method is characterized in that MMT powder and GdCl3 are mixed in water and react, MMT-Gd compound powder is obtained and reacts with DTPA in water, and the MMT-Gd-DTPA compound is obtained. The MMT-Gd-DTPA compound is obtained through hydro-thermal synthesis, the method is simple and easy to implement, the obtained compound not only has good longitudinal relaxation rate r1 and good biocompatibility, but also has good effect of adhesion to the digestive tract wall, is a better T1 magnetic resonance molecular imaging oral contrast agent and has higher clinical application potential.

The invention provides a lenvatinib (Len) liposome, a pharmaceutical composition thereof, a preparation method thereof and a prescription process optimization method. The liposome contains lenvatinib, phospholipid, an amphiphilic derivative, cholesterol and a gradient establishing substance, wherein the weight ratio of cholesterol to phospholipid is 1:2-1:10, the weight ratio of lenvatinib to phospholipid is 1:10-1:40, the weight ratio of the amphiphilic derivative to the phospholipid is 1:2-1:10, the phospholipid is glycerophospholipid or sphingomyelin, the gradient adopts a pH gradient method or an amine gradient method, and the concentration of the gradient establishing substance solution is 100-300mM. The Len liposome can actively target a tumor site through an SA-Siglec1 pathway, has the advantages of high encapsulation efficiency, high stability, high active targeting, high tumor inhibition rate and the like, and has good clinical application potential and product development value.

The invention discloses a phenol recognition SERS probe, its preparation and application, and a SERS-based general ultrasensitive immune analysis method. First, DTDBA was used to reduce chloroauric acid to prepare a phenol-responsive SERS probe, then ELISA was combined with SERS, ALP was used to label biomolecules, and ALP was used to hydrolyze its substrate PPNa to produce phenol, and the SERS probe signal caused by phenol was thus Technologies to achieve sensitive detection of biomolecules. This technology can not only overcome the shortcomings of low sensitivity of conventional enzyme-linked immunoassays, but also solve the problems of SERS detection signal enhancement and poor reproducibility. The invention has remarkable universality, can be widely used in immunoassays using ALP as an enzyme marker to measure various biomolecules, and lays a solid foundation for the development of immune technology based on SERS detection, and can be used in the fields of biology, chemical detection, medical diagnosis, etc. All have great development space and broad application prospects.

The invention relates to the field of the biochemical industry and biological extraction, and more specifically relates to a separation and extraction method of croton essential oil, and an application. The method comprises the following steps: crushing croton kernels and treating the same; adding the treated croton kernel powder into a supercritical extraction container, inputting supercritical carbon dioxide fluid in the extraction container, and extracting the same; enabling the extracted carbon dioxide fluid to enter a separator to separate, wherein the flow of the carbon dioxide is 2L / min, and the material discharged from the separator is the croton essential oil. The effective ingredients of the croton essential oil can be reserved as much as possible, the croton medicinal material resources can be sufficiently utilized, the crotin is inactivated, and the toxicity is reduced.

The invention discloses a hydroxychloroquine sulfate sustained-release microsphere for articular cavity injection and a preparation method thereof. The preparation method comprises the following steps: dissolving a surfactant in an organic solvent to form an oil phase; adding hydroxychloroquine sulfate and gelatin into water to obtain a water phase; dropwise adding the water phase into the oil phase, stirring and emulsifying to form a W / O type emulsion; transferring the W / O emulsion into an ice bath, and adding a curing agent for cross-linking curing; and then adding isopropanol for dehydration, suction filtration, washing and drying to obtain the hydroxychloroquine sulfate sustained-release microspheres for articular cavity injection. The prepared hydroxychloroquine microspheres can stably and continuously release drugs for more than 48 hours, a sustained-release preparation is obtained, the administration frequency can be reduced, the total dosage can be reduced, the compliance of patients can be improved, and the clinical application potential is good.

The invention provides a preparation method of biological ink containing PRP, and the preparation method comprises the following steps: preparing liquid hydrogel which comprises the following components in percentage by mass: 1%-30% of gelatin and 1%-10% of sodium alginate; fully mixing liquid hydrogel and PRP with the volume ratio being (9-36): 1 to be uniform, the quality standard of the PRP being as follows: based on the platelet concentration, the platelet concentration being larger than or equal to 1000 * 10<9> / L in a single platelet collection method, the platelet concentration being larger than or equal to 1 * 10<9> / L in a secondary centrifugation method, and obtaining the biological ink containing the PRP. The preparation method of the tissue organoid provided by the invention has the following advantages: (1) the bioactive components are added without changing the original physical and chemical properties of the bio-ink; and (2) the application safety and effectiveness in wound treatment are widely accepted, and excellent clinical application potential is achieved.

The invention provides a levoulifloxacin mesylate crystal. The Cu-Kalpha radiated X-ray powder diffraction pattern of the crystal has the following 2theta angel expressed peaks: 5.4-5.8 degrees, 18-18.4 degrees and 25.8-26.2 degrees. The invention also provides a preparation method of the crystal, a medical composition containing the crystal, as well as medical application of the crystal and the medical composition. The crystal provided in the invention has good reproducibility, as well as simple and rapid preparation method. Experiments prove that the crystal of the invention is better than existing crystals in terms of solubility, stability, hygroscopicity and treatment effects in vivo. The crystal in the invention can also be prepared into a plurality preparation forms for use and has good clinical application potential.

The invention relates to the field of biomedicine, in particular to a fusion protein containing a chimeric antigen receptor and its application. The fusion protein includes chimeric antigen receptor, 2A peptide, first signal peptide, Sirpα domain and IgG1 Fc in series; said chimeric antigen receptor comprises scFv region, hinge region, transmembrane domain and intracellular signal transduction Area. Sirf CAR T cells can reduce tumor burden, improve the survival rate of tumor-bearing mice, and have no obvious hematological toxicity and side effects, and have good potential for clinical application. SIRPα-Fc fusion protein can enhance the anti-apoptosis and memory phenotype of CAR T cells, and change the immune microenvironment of tumor tissue.

The invention discloses a synthetic method of a eumelanin-like nano contrast agent loaded with a therapeutic drug, which comprises the following steps: firstly, polymerizing metal salt ions as a manganese source and dopamine hydrochloride as precursor molecules to form manganese-eumelanin nano particles, and then loading a targeted drug on the manganese-eumelanin nano particles to obtain the eumelanin-like nano contrast agent. The invention also discloses an application of the eumelanin-like nano contrast agent loaded with the therapeutic drug. The eumelanin-like nano contrast agent provided by the invention has high metal loading capacity and geometric confinement effect, and shows excellent T1-T2 dual-mode MRI contrast enhancement capability; meanwhile, a neuroprotective agent curcumin is loaded on angiopep-2 functionalized and manganese-doped eumelanin nanoparticles to prepare antioxidation and anti-neuroinflammation for targeted diagnosis and treatment of traumatic brain injury and AMEC accumulated at a traumatic part, so that a long-term treatment effect on secondary brain injury is realized; the AMEC well combines a drug carrier with medical imaging, and has good clinical application potential.

The invention discloses a method for preparing a supermolecule capsule with multiple drug release stimulation and MRI radiography ability. A ferroferric oxide microcapsule is prepared as a novel multifunctional magnetic resonance noise and drug therapy medicament through a layer-by-layer self-assembly technology. Through the host-guest interaction and the Coulomb interaction of different supramolecular polymers, the medicine molecule is controllably released through the non-invasive ultraviolet radiation distributed azobenzene isomerism and the acid response Schiff base. Additionally the magnetic-targeted MRI contrast agent is obtained by encapsulating the superparamagnetic ferroferric oxide granule. The r2 relaxation rate of the microcapsule is increased by 37% to reach 125.71 mM-1S-1 compared with that of the neutral condition (92.02mM-1S-1) through the stimulation of subacidity (pH=5.6).

The invention relates to the field of biomedicine, in particular to a fusion protein of cytokine combined with chimeric antigen receptor and its application. The fusion protein includes chimeric antigen receptor, 2A peptide, IL-7, 2A peptide and CCL3 in series; the chimeric antigen receptor includes scFv region, hinge region, transmembrane domain and intracellular signal conduction region. The present invention adds two cytokines IL-7 and CCL-3 when constructing the CAR vector, and the two cytokines can enhance the anti-apoptotic effect of CAR T cells and reduce their immunosuppressive effect, thereby helping CAR T cells to enhance anti-tumor effect.

The present invention relates to a croton processing method for treatment of tuberculosis, and specific relates to a high temperature processing attenuation method of a traditional Chinese medicine croton and the role thereof in the treatment of tuberculosis. The croton processed product is obtained through three methods of high-temperature stir-frying, high temperature baking, and microwave heating. The processed croton has no toxic protein detected. LD50 increases significantly. The relative content of each component in the croton is essentially unchanged, and the croton oil content is relatively elevated. The processed croton is mixed with other traditional Chinese medicines to make pills which have significant role in resisting mycobacterium tuberculosis in vitro and in vivo. The croton processing method for treatment of tuberculosis disclosed by the invention has effects of reducing toxicity and enhancing efficacy, and has very prominent advantages compared with conventional croton processing methods such as baking, boiling, steaming, etc.

The invention provides a composition for long-acting supplement of arginine and neutralization of an acid environment for targeted immune cells and application thereof. The L-arginine sustained-release preparation consists of L-arginine, a cationic amino acid transporter 2 inhibitor and a pharmaceutically acceptable sustained-release drug storage depot carrier. According to the invention, L-arginine and a cationic amino acid transporter 2 inhibitor are co-loaded in a sustained-release drug reservoir to prepare an antitumor drug. According to the invention, by locally injecting into a tumor part, cationic amino acid transporter protein 2 of cancer cells and immunosuppressive cells is inhibited, and alkaline L-arginine is slowly released in a tumor in a long-acting manner, so that targeted tumor killing immune cell L-arginine supplementation and tumor cell and immunosuppressive cell L-arginine hunger are realized; and the alkalinity of the L-arginine is utilized to neutralize the acidic environment, so that the immunity-promoting improvement on the tumor microenvironment is completed on the two aspects of acidity and nutrition, the anti-tumor immune response can be remarkably enhanced, and the tumor treatment effect can be improved.

The invention relates to a biodegradable in-situ solidification slow-release injection, which consists of an amphiphilic block polymer, a medicament and an organic solvent, wherein the weight proportion of the amphiphilic block polymer to the medicament to the organic solvent is that amphiphilic block polymer: medicament: organic solvent is equal to 100: 1-90: 1-10,000.

The invention provides a biological 3D printing ink and a preparation method thereof. The preparation method comprises the following steps: S1: preparing a calcium hydroxide saturated solution, addinga silica nanoparticle suspension to the calcium hydroxide saturated solution, centrifuging and taking a precipitate to obtain modified nano-bioglass particles; S2: dissolving gelatin and sodium alginate in deionized water, then adding the modified nano-bioglass particles prepared in the Step S1, stirring evenly to prepare liquid hydrogel, sterilizing and equilibrating to obtain a gelatin-sodium alginate composite hydrogel; S3: dissolving solid calcium chloride powder in deionized water to prepare a calcium chloride solution, and sterilizing to obtain a cross-linking agent; and S4: adding thecross-linking agent to the gelatin-sodium alginate composite hydrogel for cross-linking to obtain the biological 3D printing ink. The biological 3D printing ink prepared by the invention has not onlysignificantly improved mechanical properties but also good gelling performance, rich pores and good cell-carrying potential.

The invention discloses a methionine-polyesteramide high-molecular polymer with ROS responsiveness and application of the methionine polyesteramide high-molecular polymer. The structural formula of the methionine polyesteramide high-molecular polymer is shown as a formula (I). The methionine-based polyesteramide polymer material is synthesized by taking methionine, diacid containing different methylene numbers, diol and the like as raw materials through melt phase polycondensation reaction, the method is high in repeatability and simple and convenient to operate, the used raw materials are non-toxic, economical and easy to obtain, the obtained high-molecular polymer material has excellent biosafety and biodegradability, can be used as a drug carrier, can be controllably released in a high-concentration H2O2 environment, effectively solves the problems of poor stability, poor biosafety, weak targeting property and the like of nano-drugs, and has a good prospect in biomedical applications such as tumor treatment and the like.