46results about How to "Good potential for clinical application" patented technology

The invention discloses a phenol recognition SERS probe, its preparation and application, and a SERS-based general ultrasensitive immune analysis method. First, DTDBA was used to reduce chloroauric acid to prepare a phenol-responsive SERS probe, then ELISA was combined with SERS, ALP was used to label biomolecules, and ALP was used to hydrolyze its substrate PPNa to produce phenol, and the SERS probe signal caused by phenol was thus Technologies to achieve sensitive detection of biomolecules. This technology can not only overcome the shortcomings of low sensitivity of conventional enzyme-linked immunoassays, but also solve the problems of SERS detection signal enhancement and poor reproducibility. The invention has remarkable universality, can be widely used in immunoassays using ALP as an enzyme marker to measure various biomolecules, and lays a solid foundation for the development of immune technology based on SERS detection, and can be used in the fields of biology, chemical detection, medical diagnosis, etc. All have great development space and broad application prospects.

The invention relates to the field of biomedicine, in particular to a fusion protein containing a chimeric antigen receptor and its application. The fusion protein includes chimeric antigen receptor, 2A peptide, first signal peptide, Sirpα domain and IgG1 Fc in series; said chimeric antigen receptor comprises scFv region, hinge region, transmembrane domain and intracellular signal transduction Area. Sirf CAR T cells can reduce tumor burden, improve the survival rate of tumor-bearing mice, and have no obvious hematological toxicity and side effects, and have good potential for clinical application. SIRPα-Fc fusion protein can enhance the anti-apoptosis and memory phenotype of CAR T cells, and change the immune microenvironment of tumor tissue.

The invention discloses a method for preparing a supermolecule capsule with multiple drug release stimulation and MRI radiography ability. A ferroferric oxide microcapsule is prepared as a novel multifunctional magnetic resonance noise and drug therapy medicament through a layer-by-layer self-assembly technology. Through the host-guest interaction and the Coulomb interaction of different supramolecular polymers, the medicine molecule is controllably released through the non-invasive ultraviolet radiation distributed azobenzene isomerism and the acid response Schiff base. Additionally the magnetic-targeted MRI contrast agent is obtained by encapsulating the superparamagnetic ferroferric oxide granule. The r2 relaxation rate of the microcapsule is increased by 37% to reach 125.71 mM-1S-1 compared with that of the neutral condition (92.02mM-1S-1) through the stimulation of subacidity (pH=5.6).

The invention provides a lactic levorotatory ulifloxacin crystal. According to the lactic levorotatory ulifloxacin crystal, an X-ray powder diffraction map of Cu-K alpha radiation has the following peaks expressed by 2 theta degrees: 5.1+ / -0.2 degrees, 10.3+ / -0.2 degrees and 15.5+ / -0.2 degrees. The invention also provides a method for preparing the lactic levorotatory ulifloxacin crystal, a medicinal composition containing the crystal and application of the lactic levorotatory ulifloxacin crystal and the medicinal composition to pharmacy. The lactic levorotatory ulifloxacin crystal has high reproducibility, and the preparation method is simple and quick. Experiments prove that the solubility, stability, hygroscopicity and an in-vivo treatment effect of the lactic levorotatory ulifloxacin crystal are superior to those of the conventional crystal of the compound, and the lactic levorotatory ulifloxacin crystal can also be prepared into various formulations, and has a good clinical application potential.

The invention relates to the field of biomedicine, in particular to a fusion protein of cytokine combined with chimeric antigen receptor and its application. The fusion protein includes chimeric antigen receptor, 2A peptide, IL-7, 2A peptide and CCL3 in series; the chimeric antigen receptor includes scFv region, hinge region, transmembrane domain and intracellular signal conduction region. The present invention adds two cytokines IL-7 and CCL-3 when constructing the CAR vector, and the two cytokines can enhance the anti-apoptotic effect of CAR T cells and reduce their immunosuppressive effect, thereby helping CAR T cells to enhance anti-tumor effect.

The present invention relates to preparation process of biodegradable reverse temperature sensitive material used for medicine conveying system, cell embedment, biological tissue engineering and medical equipment. The present invention adopts biodegradable polysaccharide material, such as gelatin, xanthan gum, chitosan and xylan as main skeleton material, and introduces interpenetrating network assistant polyenol to form double crosslinked or hybridized gel network structure. The preparation process includes crosslinking polysaccharide and polyenol in water solution with crosslinking agent, co-dissolving with polyhydric phosphate in ice bath, and regulating pH to 6.8-7.4 with saturated disodium biphosphate solution after reaction, so as to obtain biodegradable sol-gel conversion system for injection. The material can form gel at 37 deg.c, is temperature sensitive and the added polyenol has the functions of raising hydrophobic performance, raising gel strength, etc.

The invention provides a biological 3D printing ink and a preparation method thereof. The preparation method comprises the following steps: S1: preparing a calcium hydroxide saturated solution, addinga silica nanoparticle suspension to the calcium hydroxide saturated solution, centrifuging and taking a precipitate to obtain modified nano-bioglass particles; S2: dissolving gelatin and sodium alginate in deionized water, then adding the modified nano-bioglass particles prepared in the Step S1, stirring evenly to prepare liquid hydrogel, sterilizing and equilibrating to obtain a gelatin-sodium alginate composite hydrogel; S3: dissolving solid calcium chloride powder in deionized water to prepare a calcium chloride solution, and sterilizing to obtain a cross-linking agent; and S4: adding thecross-linking agent to the gelatin-sodium alginate composite hydrogel for cross-linking to obtain the biological 3D printing ink. The biological 3D printing ink prepared by the invention has not onlysignificantly improved mechanical properties but also good gelling performance, rich pores and good cell-carrying potential.