36results about How to "Enhanced SERS signal" patented technology

The invention discloses a phenol recognition SERS probe, its preparation and application, and a SERS-based general ultrasensitive immune analysis method. First, DTDBA was used to reduce chloroauric acid to prepare a phenol-responsive SERS probe, then ELISA was combined with SERS, ALP was used to label biomolecules, and ALP was used to hydrolyze its substrate PPNa to produce phenol, and the SERS probe signal caused by phenol was thus Technologies to achieve sensitive detection of biomolecules. This technology can not only overcome the shortcomings of low sensitivity of conventional enzyme-linked immunoassays, but also solve the problems of SERS detection signal enhancement and poor reproducibility. The invention has remarkable universality, can be widely used in immunoassays using ALP as an enzyme marker to measure various biomolecules, and lays a solid foundation for the development of immune technology based on SERS detection, and can be used in the fields of biology, chemical detection, medical diagnosis, etc. All have great development space and broad application prospects.

The invention relates to a method for detecting the content of dioxane in children cosmetics. The method comprises the following steps: (1) pretreating a sample to be detected so as to form a sample solution; (2) uniformly mixing the sample solution with prepared nanometer silver colloid so as to form a sample mixed solution; (3) dropping the sample mixed solution on a substrate, and naturally airing, thus obtaining a detection sample; (4) detecting the detection sample by adopting surface-enhanced Raman scattering, thus obtaining an SERS (surface enhanced Raman spectrum) spectrogram of the detection sample; and (5) comparing the obtained SERS spectrogram of the detection sample with an SERS spectrogram of a dioxane standard solution, and calculating so as to obtain the content of the dioxane in the detection sample. The method is simple, convenient and fast to operate and can be used for rapidly carrying out qualitative and quantitative analysis on the content of the dioxane in children cosmetics.

The invention discloses a surface enhanced Raman scattering probe based on a carbon nanotube and a preparation method thereof. By adopting the structural characteristics of the carbon nanotube, the surface enhanced Raman scattering signal intensity of the probe is raised. The probe comprises a core layer, a coating layer adsorbing around the core layer and a surface enhanced Raman scattering marker adsorbing around the coating layer, wherein the core layer comprises a carbon nanotube coated with a layer of positive polypropylene amine hydrochloride, and the coating layer is a metal nanoparticle layer composing of metal nanoparticles. According to the invention, the surface enhanced Raman scattering probe with a specific structure is prepared by using the metal nanoparticles as a surface enhanced Raman scattering substrate and using the carbon nanotube as a loading template; under the irradiation of excitation lights, the probe has surface enhanced Raman scattering signals with high signal to noise ratio.

The invention relates to a preparation method for a graphene-based surface enhanced Raman scattering (SERS) probe. Compared with the traditional SERS probe using metal nanoparticle sol as an enhanced substrate, the SERS probe adopts a graphene-metal nanoparticle composite structure as a surface enhanced Raman substrate, wherein the SERS signal is obviously enhanced and the metal nanoparticles on the surface of the graphene are uniform and controllable. The main reason is that as gold nanoparticles exist on the surface of a silicon wafer dispersively, sufficient 'hot points' cannot be generated among the particles, and the gold nanoparticles gather on the surface of graphene oxide, the electromagnetic enhanced effect is achieved and stronger SERS effect is achieved.

The invention relates to a spectral method capable of exciting surface-enhanced Raman spectroscopy (SERS) in a long range surface plasmon mode. The method comprises the following steps of: constructing a buffer layer, a metal layer and a protective layer on the bottom surface of a prism to form a long range surface plasmon resonance (LRSPR) device; placing the LRSPR device with multilayer structure under the irradiation of a laser source, and adjusting the incident angle of the laser source to an LRSPR angle; and in a specific incident direction, generating the LRSPR, so that the electric magnetic field on the surface of a metal is enhanced, and the excitation process of the surface-enhanced Raman spectroscopy of a detected object of a deeper area in a sample layer is completed. Because the long range effect has deeper penetrating effect, the construction of the protective layer on the surface of the metal layer becomes possible. The transduction membrane made of chemically inert goldor platinum is changed into a silver membrane with lower cost, oxidation resistance and better enhancing effect. The LRSPR-mechanism-based SERS detection method has great significance.

The invention discloses a silver cube / flower-shaped silica core-shell nanomaterial and its preparation method and application. The preparation method comprises mixing and stirring an aqueous solution of silver nitrate, an aqueous solution of cetyltrimethylammonium bromide and an aqueous solution of glucose into a hydrothermal reaction kettle, and after heating and reacting, centrifuging to collect precipitates to obtain silver cubic nanoparticles; Cubic nanoparticles are dissolved in a mixed solution composed of water, ethanol, cetyltrimethylammonium bromide and ammonia water, after stirring, add 4-mercaptophenylacetic acid ethanol solution and polyacrylic acid ethanol solution, and after stirring, add orthosilicic acid Ethyl ester, after 6 hours of reaction, just collect the precipitate after centrifugation. The advantage is that it has good stability and biocompatibility. The mesoporous structure can load more Raman molecules and enhance the surface Raman scattering signal intensity. .

The invention discloses an integrated nano-reagent for cancer diagnosis and treatment, including a miRNA identification probe prepared by modifying a Y-shaped DNA structure on the surface of AuNP, and a miRNA identification probe prepared by modifying a double-stranded DNA linker and a Raman molecule on AuNP For the obtained SERS probe, the invention also discloses the preparation method and application of the above-mentioned integrated reagent for cancer diagnosis and treatment. The integrated nano-reagent for cancer diagnosis and treatment of the present invention, driven by ATP in cancer cells, the specially designed Y-shaped DNA structure and double-stranded DNA linker on AuNP will undergo conformational transformation triggered by miRNA-106a, releasing miRNA- 106a and cyclic amplification, triggering the SERS signal to significantly enhance the recognition of cancer cells; at the same time, the DNAzyme generated and amplified by the conformational switch can catalyze the cleavage of Survivin mRNA and c-Jun mRNA, realizing effective dual gene silencing therapy.

The invention provides a Raman sensing analysis method for detecting enterotoxin, which belongs to the technical field of optical analysis. Comprise the following steps: cesium bromide (CsBr) and lead bromide (PbBr 2 ) powder doped in mesoporous silica (MSNs) to prepare sterically hindered CsPbBr via high-temperature desolvation 3 @MSNs. Ag islands were grown on the Au core via the ligand 2‑mercaptobenzimidazole‑5‑carboxylic acid (MBIA) to synthesize Au‑Ag Janus NPs. Furthermore, the ligand MBIA acts as a Raman beacon molecule, exhibiting a very unique and stable Raman signal. Au‑Ag Janus NPs and CsPbBr 3 @MSNs can greatly amplify the SERS signal through the composite material formed by antigen-antibody. The Raman signal of MBIA is used as the detection signal to avoid additional modification of the Raman beacon. The detection method of the invention has high sensitivity and strong specificity.