41results about How to "Detection without interference" patented technology

The invention relates to a detection method, in particular to a method for detecting sulfur dioxide in plant-derived food, and belongs to the technical field of food detection. The method provided by the invention comprises steps of: using plant-derived food as a test material; crushing the raw material, and adding hydrochloric acid for nitrogen filling distillation, so as to release of sulfur dioxide, which is then adsorbed by a formaldehyde solution; adding sodium hydroxide and Pararosaniline hydrochloride to form a amaranth complex; determining absorbance value at a wavelength of 577nm; and calculating the content of sulfur dioxide of the plant-derived food according to the absorbance value. The plant-derived food comprises grains, beans, nuts, edible fungus, fruits, vegetables and products thereof. The method replaces a poisonous reagent of a sodium tetrachloromercurate absorption liquid or a heavy metal pollution reagent of a lead acetate absorption liquid with the formaldehyde absorption solution, and can reduce the environmental pollution and damage to the testing personnel. The absorption liquid of the sample in the invention is colorless and transparent, has no interference on the determination of colored sample, and is suitable for detection of all kinds of plant-derived food.

The invention discloses a fluorescent probe which has the following structural formula, wherein R1, R2, R3, R4 and R5 are selected from C1-C18 alkyl group, aryl group, ester group or ether group; n and m are selected from 0-8; Ar is selected from naphthalene, anthracene or pyrene; and X<-> is selected from F<->, Cl<->, Br<-> or I<->. The invention also discloses a synthesis method and application of the fluorescent probe. The fluorescent probe can be used for detecting heparin in blood, and has the advantages of simple molecular structure, short response time, high detection sensitivity and simple synthesis method.

The invention discloses polystyrene mercury ion fluorescence recognition materials. The structural formula of the polystyrene mercury ion fluorescence recognition materials is as follows. The invention also discloses a preparation method of the functionalized polystyrene mercury ion fluorescence recognition materials. The preparation method comprises the following specific implementation steps of, preparing 5-amino-2-(4'-hydroxy-3'-(2-(2''-ethylsuleenyl ethyl) amino) phenyl-5-amino benzo [d] oxazole (VII), preparing aldehyde polystyrene microspheres (IX) and performing reaction preparation on the compounds (VII) and (IX) obtained through preparation to obtain the functionalized polystyrene mercury ion fluorescence recognition materials (X). The detection sensitivity of the polystyrene mercury ion fluorescence recognition materials is greatly improved due to the molecular wire effect of a polymer and the detection limit is 0.006 micromole every liter. The polystyrene mercury ion fluorescence recognition materials can enter cells and can be applied to the detection on mercury ions in the cells.

The invention provides a kit for co-separation of (deoxyribonucleic acid) and RNA (ribonucleic acid) from FFPE (formalin fixed and paraffin embedded tissues) as well as a method. The kit can be used for simultaneously extracting DNA and RNA from an FFPE sample, and has the advantages that not only can nucleic acid extraction be performed simultaneously, time is saved, and the extraction is economical and fast, but also the sample size can be saved, the problem of sample lack in a test can be solved effectively, and further, identical source of the DNA and the RNA used in study can be guaranteed. The kit and the method have the advantages that the operation is simple and direct, the repeatability is high, the yield is high, and enough DNAs and RNAs can be obtained simultaneously only through 2-5 pieces of tumor tissue sections; the obtained nucleic acids are high in purity and has no interference to follow-up detection; and compared with the common kit, the kit has remarkable advantages.

The invention discloses a rapid colorimetric detection method for acetamiprid based on nano-enzyme catalysis. The rapid colorimetric detection method comprises the following steps: A, drawing a standard curve for the variation of the absorbency of acetamiprid; B, based on the standard curve for the variation of the absorbency of acetamiprid, establishing a linear regression equation for the relationship between the concentrations of acetamiprid and the absorbency of acetamiprid; C, preparing a to-be-detected solution C with an unknown acetamiprid concentration; and D, subjecting the to-be-detected solution C to absorption spectrum scanning so as to measure the absorbency of acetamiprid at a wavelength of 735 nm, calculating a difference between the absorbency of the to-be-detected solution C and the absorbency of an A2 product, and substituting the difference into the linear regression equation so as to obtain the concentration of acetamiprid in the to-be-detected solution C. The method does not require professional operations, and has the advantages of simple operation, rapid detection, low cost, intuitive test results, suitability for on-site detection, etc.

The invention discloses a method for preparing a molecularly imprinted membrane detecting device for tetracycline. The detecting device comprises a reactor, wherein the reactor is provided with a working electrode and a reference electrode; the working electrode is the tetracycline molecularly imprinted membrane electrode which comprises an electrode matrix; the reference electrode is a saturated calomel electrode; the electrode matrix is made by electrically depositing titanium sheets with micro-nano structure platinum particles; and the machining process of the tetracycline molecularly imprinted electrode includes: mixing materials such as template molecules and polymerzing on the electrode matrix through a photopolymerization method after ultrasound dissolve; dipping the electrode matrix in a sol, then eluting the template molecules therein, thereby preparing the electrode. The invention utilizes the identity of molecularly imprinted polymers (MIPs) to identify tetracycline special contamination without large-sized test apparatuses, has low test cost and simple and portable operation and has obvious advantages in on-site detection or portable detection aspects. The test technology can be widely applied to detecting tetracycline antibiotic special contamination contained in food, waste water of pharmaceutical factories, chemical plants, dye factories and the like.

The invention discloses a method for naked eye discernible colorimetric detection on dimethoate. The method comprises the following steps: A, drawing a dimethoate absorbancy variation standard curve;B, according to the dimethoate absorbancy variation standard curve, establishing a linear regression equation according to the mutual relationship between dimethoate concentration and dimethoate absorbancy values; C, preparing a solution C to be detected with unknown dimethoate concentration; D, performing absorption spectrum scanning on the solution C, testing the absorbancy value of the solutionC at the wavelength of 450nm, calculating an absorbancy value difference of the solution C and a product A, and substituting the absorbancy value difference into the linear regression equation, thereby obtaining the concentration of dimethoate in the solution C. The method needs no large-size instrument equipment in the application process, has the advantages of high detection sensitivity, good selectivity, simple and rapid operation, naked eye discrimination and the like and can be widely applied to rapid detection on dimethoate residues in agricultural products.

The invention discloses an electrochemical biosensor based on polypeptide analog with the electrocatalytic activity for acetylcholin esterase detection. The biosensor for acetylcholin esterase detection is characterized by comprising the following steps: (1) intensively stirring and uniformly mixing silver nitrate, L-cysteine and secondary distilled water on a stirrer, and incubating in an oscillator; (2) electrodepositing graphene onto a bare glassy carbon electrode by utilizing an electrochemical method, mixing 0.05 weight percent of a Nafion solution with the solution obtained in the step (1), and dropwise coating GO / GCE to obtain an electrochemical biosensor based on the polypeptide analog with electrocatalytic activity (CP / GO / GCE); (3) performing acetylcholin esterase activity detection, namely catalyzing acetylcholine in one step to generate H2O2, uniformly mixing acetylcholine, AchE and choline oxidase, and incubating to generate H2O2, and detecting the solution with the sensorprepared in the step (2) with good specificity, high sensitivity, high detection speed, accurate and reliable result; and (4) performing AChE inhibitor malathion detection, namely uniformly mixing acetylcholine, AChE, malathion and choline oxidase, incubating, and detecting the solution with the sensor prepared in the step (2). The biosensor has the advantages of good specificity, high sensitivity, high detection speed and accurate and reliable result.

The invention discloses a laser-induced breakdown spectroscopy electrochemical combined heavy metal detection method. According to the method, heavy metal ions are reduced to heavy metal through an electrochemical system and an electrochemical analysis method and then enriched on working electrodes; and contents of heavy metal pollutants are measured in situ by utilizing a laser-induced breakdownspectroscopy system. According to the method, laser-induced breakdown spectroscopy and electrochemistry are combined so that contents of heavy metal ions in complicated real environment can be conveniently and rapidly detected in situ; the interference problems of traditional electrochemistry and the height detection limit problems of traditional laser-induced breakdown spectroscopy are overcome,and the contents of the heavy metal ions can be detected via high sensitivity without interferences; and electrode surface state changes in the electrochemical analysis process can be represented in situ so as to disclose interference mechanisms of electrochemical behaviors among heavy metal pollutants.

The invention discloses a method for simultaneously determining 4-amino-2-(2,6-dioxo-3-piperidyl)isoindoline-1,3-dione and related substances thereof. According to the method, the 4-amino-2-(2,6-dioxo-3-piperidyl)isoindoline-1,3-dione and the related substances thereof are separated through high performance liquid chromatography, wherein a chromatographic column is bonded by octadecyl silica gel; a mobile phase is a methanol:acetic acid aqueous solution. The flow velocity in the high performance liquid chromatography is 0.5 to 1.2ml / min. The detection wavelength in the high performance liquid chromatography is 226nm. The method is simple, convenient and practical, can be used for quickly achieving the aims of simultaneously determining and separating the 4-amino-2-(2,6-dioxo-3-piperidyl)isoindoline-1,3-dione and the related substances thereof, can be applied to determination of molecular weights of unknown impurities through liquid chromatography-mass spectrometry (LC-MS), provides a reliable basis for structure inference of the unknown impurities, and facilitates quality control of raw materials and preparations of the 4-amino-2-(2,6-dioxo-3-piperidyl)isoindoline-1,3-dione.

The invention relates to a method for detection of a physiological matter, and in particular, relates to a method for quantitative detection of L-cysteine by using a glucose / oxygen biofuel cell. A self powder supply property of the glucose / oxygen biofuel cell is utilized, at the same time, an open circuit electric potential of the cell is inhibited in the presence of Cu<2+>, Cu-S bonds are formed by the L-cysteine and Cu<2+>, and according to the change of the open circuit electric potential along with the concentration of the L-cysteine, the L-cysteine in a to-be-measured sample is quantitatively detected. With utilization of the principle, a linear relationship between the change quantity of the open circuit electric potential and the concentration of the L-cysteine is established, and high-sensitivity detection of the L-cysteine is realized. The method has the advantages of simple operation, high sensitivity and good specificity, and is applicable in detection of the L-cysteine in the fields of food industry, physiological processes and clinical medicine.

The invention relates to preparation and application of a novel fluorescent probe and test paper for hydrazine hydrate detection, and belongs to the technical field of analytical chemistry. The fluorescent probe takes a benzothiazole mother nucleus as a parent and acetyl as a recognition site, and the chemical structure of the fluorescent probe is shown as a formula (I). When the fluorescent probereacts with hydrazine hydrate, acetyl is hydrolyzed into hydroxyl, a fluorescent substance with high fluorescence emission capacity is generated in a PBS-CH3CN (10 mM, 3: 7, v / v, pH=7.4) buffer solution, fluorescence is changed from blue to green, and ratio type detection of hydrazine hydrate is realized. Besides, the fluorescent probe only responds to hydrazine hydrate, does not respond to smallmolecules such as methylamine, ethylamine, ethylenediamine, butylamine, dodecylamine, n-hexylamine, octadecylamine, aniline, Cys and GSH, and can realize specific recognition of hydrazine hydrate. Inaddition, the fluorescent probe provided by the invention can also be used for rapid detection of hydrazine hydrate in the environment, and has a very good application prospect. Formula I is shown.

The invention relates to a rare earth-organic porous material, a preparation method thereof and application thereof in the detection of nerve disease marker glutamic acid. Through the technical scheme, the preparation method comprises the following steps: dissolving Ln(NO3)3.6H2O, hydroxyterephthalic acid and 2-fluorobenzoic acid into a mixed solution of an organic solvent, water and nitric acid;sealing after ultrasonic uniformity, putting the mixture in a baking oven at the temperature of 110 DEG C for 3 d; naturally cooling to room temperature, washing, filtering and drying to obtain the rare earth-organic porous material. The rare earth-organic porous material prepared in the invention has obvious fluorescent response to the nerve disease marker glutamic acid, rare earth luminescence is taken as an interior label, the luminescence of organic molecules is taken as a detection signal, high-selective detection for glutamic acid can be realized, and then the material can be used for early prevention and diagnosis of nerve diseases.

The invention relates to the technical field of single-chip microcomputers, in particular to a micro-fluidic chip for detecting micro-pollutants, which is characterized by comprising a first acrylic plate and a second acrylic plate, nine engraved grooves are formed in the first acrylic plate and used for storing detection liquid and providing detection space, the first groove to the seventh grooveare used for storing detected objects and objects to be detected, the eighth groove is used for storing waste liquid, and the ninth groove is used for reacting. The device is small, exquisite, portable, simple in structure, low in cost, high in stability, long in service lifetime and free of interference to detection.

The invention discloses a detection method of pimavanserin tartrate. The detection method comprises the following steps that (1) a standard reference solution of the pimavanserin tartrate is prepared;(2) a sample solution for test is prepared; (3) a reversed-phase high performance liquid chromatography chromatogram of the pimavanserin tartrate in the standard reference solution is measured by using a reversed-phase high performance liquid chromatography, and degree of separation between the pimavanserin tartrate and impurities adjacent to the pimavanserin tartrate is enabled to be greater than or equal to 1.5; (4) a chromatogram of the sample solution for test is measured by using the reversed-phase high performance liquid chromatography; and (5) the content of the pimavanserin tartrate in the sample solution for test is determined by using an external standard method. According to the detection method of the pimavanserin tartrate, the content of the pimavanserin tartrate can be measured effectively, and no interference exists in the detection, the specialization is good, the sensibility is high, the stability is good, the degree of precision is high and the method is reliable (the recovery ratio of the pimavanserin tartrate is greater than 90%); and meanwhile, the operation is relatively simple, and the quality of subsequent pimavanserin tartrate bulk pharmaceutical chemicalsand preparations is guaranteed.

The invention discloses a fluorescent colorimetric chemical-sensitive material as well as a synthesis method and application of the material. The material has the following structural formula shown in the specification, wherein R<1>, R<2>, R><3>, R<4>, R<5>, R<6>, R<7>, R<8> and R<9> are selected from hydrogen atom, C1-18 alkyl, aryl, ester group or ether group; Ar is selected from thiophene, pyrrole, benzene, naphthalene, anthracene, pyrene, indole, coumarin, fluorescein, carbazole, rhodamine, cyano dye, fluorene or quinoline; m is 1-1000; n is 1-1000; and X<-> is selected F<->, Cl<->, Br<->, or I<->. The compound can be used for detecting existence and content of lipopolysaccharide.

The invention, which relates to the technical field of composite material preparation and bio-detection products, discloses an enzyme immobilization matrix prepared by using a mesoporous-silica-coatedgold nanorod(AuNR@mSiO2) as a dopant and application thereof to an immobilized enzyme and in biological detection. A AuNR@mSiO2 solution is mixed with titanium dioxide hydrogel precursor liquid and then the mixed solution is mixed with a chitosan solution, and coating of the final mixed solution is carried out to obtain AuNR@mSiO2-doped 'titanium dioxide-chitosan' enzyme immobilization matrix. The acetylcholin esterase electrode with AuNR@mSiO2 doped has the substantially improved detection sensitivity to the acetylcholin esterase inhibitor being an organophosphorus insecticide. The doping process is simple in operation; the prepared enzyme immobilization matrix and enzyme electrode product have high usage stability and storage stability; the enzyme substrate and enzyme inhibitor detection sensitivity is high; and the precision and the recovery rate are high; and thus the enzyme immobilization matrix has the broad application prospects.

The invention discloses a method for analyzing 2-chlorophenylglycine methyl ester tartrate and impurities. The main impurities are phenylglycine methyl ester and m-chlorophenylglycine methyl ester. A high performance liquid chromatography method is adopted, an acid aqueous solution and an organic solvent are used as mobile phases, gradient elution is carried out on a sample solution of the chlorophenylglycine methyl ester, and the method is rapid, simple, accurate, good in repeatability and suitable for control and impurity research of the chlorophenylglycine methyl ester tartrate and impurities.

The invention belongs to the field of detection, and provides a method for rapidly identifying and detecting a main allergen, tropomyosin, in an aquatic product based on an intermediate infrared spectrum technology. By obtaining an intermediate infrared spectrogram of a detected sample, through second-order derivation, the infrared spectrogram characteristic peak of the tropomyosin and the infrared spectrogram of the detected sample are compared, and thus rapid qualitative identification and quantitative detection of the tropomyosin are achieved. The tropomyosin can be rapidly detected withoutantibodies, the purposes of rapid qualitative identification and quantitative detection can further be achieved, and meanwhile the requirement of non-destructive food is met.

The invention belongs to the technical field of fine chemical engineering, particularly relates to a dual-photon fluorescence sodium ion probe and a synthesis method and application thereof. The probeis prepared by using distyrene dicyanide as the parent of the dual-photon fluorescence. The probe has small molecule volume and good selectivity to sodium ions. Oher ions other than sodium ions suchas zinc, potassium, calcium, magnesium, manganese, mercury and the like have no interference to the detection. The sodium ions of micro-molar concentration can be detected. The fluorescence intensityof the probe molecular is reduced after complexation with sodium ions, and the sodium ion content can be detected by double-photon fluorescence. The double-photon fluorescence microscopic imaging experiment shows that the probe has good permeability to cells or tissues such as fibroblast, has no toxic and side effect on the cells, is particularly suitable for detecting the concentration change ofsodium ions in the cell and the detection of the distribution of the mercury ions, and has great application value in the field of biology and medicine.

The invention discloses a test paper for divalent manganese ions as well as a preparation method and application thereof. The preparation method of the test paper comprises steps that: a) sodium carboxymethylcellulose (CMC-Na) is used to react with TbCl3 to prepare CMC / Tb3 + complex; b) purified CMC / Tb3 + complex is prepared into solution, and is coated on non-fluorescent paper, and then dried. The tested solution is dripped on the test paper, and the test paper is observed under ultraviolet light, and then whether the test paper contains divalent manganese ions and the corresponding concentration range thereof can be determined; and at last, the detection limit is that the manganese ion is more than 0.0050 mg / L. The test paper for the divalent manganese ions is simple and convenient to apply and operate, high in sensitivity and capable of achieving rapid detection.

The invention discloses a detection method and application of brexpiprazole related substances. According to the detection method of the brexpiprazole related substances, the brexpiprazole related substances such as an intermediate 7-hydroxy-1H-quinoline-2-ketone (intermediate 1), 7-(4-chlorobutoxy)-1H-quinoline-2-ketone (intermediate 2) and 1-(benzo [b] thiophene-4-yl) piperazine hydrochloride (intermediate 3) and an impurity 1-bromo-4-chlorobutane can be detected at one time; the separation degree is good, the detection time is short, and the elution procedure is simple. The method is applied to preparation of brexpiprazole, the quality of each intermediate can be controlled, and the product quality can be more effectively controlled.

The invention discloses a CdS nanoparticle-based nano developing probe as well as a preparation method and an application thereof. The nano developing probe is of a core-shell structure; the core consists of CdS nanoparticles; and the shell consists of cobalt-polysulfide complex. The developing probe is capable of rapidly and sensitively making developing response on copper ions, the response time is shorter than 30 seconds, the naked eye developing linear range is 5-5mM, and the detection limit is 1mM. The nano probe is good in detection selectivity on detection of copper ions, and the existence of co-existing metal ions has no interfere to the detection. The nano developing probe can have conspicuous spectral change when the ultraviolet visible spectrum is 10nMCu<2+> in the presence of ions, and a peak absorbing peak is generated at 294nm. When the copper ion concentration is micromole, another novel absorbing peak is generated at the part near 500nm, thus being highly consistent to developing reaction. An idea is provided for a semiconductor nano material as a novel nano developing agent for detecting heavy metal ions.

The invention discloses a paint concentration detecting device which comprises a machine body, wherein an air inlet pipe is fixed to the upper surface wall of the machine body; a warning light, a liquid crystal display, a control button and an alarm are arranged on the front surface wall of the machine body from top to bottom in sequence; an induced draft fan is arranged inside the machine body; afan impeller arranged inside the induced draft fan is fixedly connected with a main shaft of an air guiding motor; the air guiding motor and the machine body are fixedly connected by a motor fixing frame; an induced draft pipe fixed on the right surface wall of the induced draft fan and a color developing agent solution bottle are tightly connected by using a rubber plug; an infrared laser component arranged on the left side of the color developing agent solution bottle and the machine body are fixedly connected by an infrared laser component fixing bracket. The infrared laser component is arranged inside the paint concentration detecting device for irradiating a color developing agent solution with an infrared laser, so that the detection time is short, the reaction is carried out in onestep and the result is accurate, and other gases do not interfere with the detection.

The invention discloses a method for simultaneously measuring the content of three bisphenol substances, which comprises the steps of filtering a sample solution by an aqueous microporous membrane, adding an extractant and an emulsifier, eddying to form a uniform emulsion, adding hydrochloric acid for centrifugal phase splitting after standing, and taking an upper extracted phase to measure the content of bisphenol A, bisphenol AF and tetrabromobisphenol A by adopting high performance liquid chromatography. According to the invention, the extractant is binary or ternary mixture of n-caprylic acid, nonanoic acid, capric acid, undecanoic acid and lauric acid. The emulsifier is ammonium hydroxide or sodium hydroxide. The mixture of various aliphatic acids is taken to prepare an eutectic solvent serving as the extractant, alkali is taken as the emulsifier for the first time, the extracted object is fully contacted with the extractant, the extraction is completely carried out, and the content of the three low-content bisphenol substances in food and food packaging materials can be accurately detected simultaneously. The method has the characteristics of fast and uniform emulsion formation, quick and complete phase splitting, high efficiency, low toxicity, simple operation, high sensitivity, good reproducibility and the like.

The invention relates to a detection method for a spray for tendon and bone injury, which is used for qualitative identification of icariin in the product, content determination of the icariin in the product by high performance liquid chromatography, and determination by gas chromatography. The content of menthol in the product is determined by high performance liquid chromatography; the method for qualitative and quantitative detection of Epimedium and the quantitative detection of menthol and Chishan powder are added in the present invention relative to the existing standard, and further The quality of the product is effectively controlled; the method of the present invention shows that the instrument precision and linear relationship are good, the specificity is strong, and the stability and repeatability are good.

The invention discloses a method for detecting pimavanserin tartrate. The method comprises the following steps: 1) preparing a standard reference substance solution of pimavanserin tartrate; 2) preparing a test sample solution; 3) using reverse-phase high performance liquid chromatography to measure pimavanserin tartrate in the standard reference substance solution The reversed-phase high-performance liquid chromatogram, make the degree of separation ≥ 1.5 between pimavanserin tartrate and its adjacent impurities; 4) use reversed-phase high-performance liquid chromatography to measure the chromatogram of test sample solution; 5) utilize external standard method to determine the content of pimavanserin tartrate in the test sample solution. The method can effectively measure the content of pimavanserin tartrate, and the detection has no interference, good specificity, high sensitivity, good stability, high precision and reliable method (the recovery rate of pimavanserin tartrate is greater than 90%) , while the operation is relatively simple, which provides a guarantee for the quality of subsequent pimavanserin tartrate raw materials and preparations.

The invention discloses a molecularly imprinted membrane detecting device for tetracycline, which comprises a reactor, wherein the reactor is provided with a working electrode and a reference electrode; the working electrode comprises an electrode matrix which is formed by electrodepositing a platinum grain titanium sheet of a micro-nano structure; the reference electrode is a saturated calomel electrode; and the working electrode is a tetracycline molecularly imprinted membrane electrode. The invention also discloses the preparation and detection method of the molecularly imprinted membrane detecting device. The invention utilizes the identity of molecularly imprinted polymers (MIPs) to identify tetracycline special contamination without large-sized test apparatuses, has low test cost andsimple and portable operation and has obvious advantages in on-site detection or portable detection aspects. The test technology can be widely applied to detecting tetracycline antibiotic special contamination contained in food, waste water of pharmaceutical factories, chemical plants, dye factories and the like.

The invention, which relates to the technical field of composite material preparation and bio-detection products, discloses an enzyme immobilization matrix prepared by using a mesoporous-silica-coatedgold nanorod(AuNR@mSiO2) as a dopant and application thereof to an immobilized enzyme and in biological detection. A AuNR@mSiO2 solution is mixed with titanium dioxide hydrogel precursor liquid and then the mixed solution is mixed with a chitosan solution, and coating of the final mixed solution is carried out to obtain AuNR@mSiO2-doped 'titanium dioxide-chitosan' enzyme immobilization matrix. The acetylcholin esterase electrode with AuNR@mSiO2 doped has the substantially improved detection sensitivity to the acetylcholin esterase inhibitor being an organophosphorus insecticide. The doping process is simple in operation; the prepared enzyme immobilization matrix and enzyme electrode product have high usage stability and storage stability; the enzyme substrate and enzyme inhibitor detection sensitivity is high; and the precision and the recovery rate are high; and thus the enzyme immobilization matrix has the broad application prospects.