The invention belongs to the technical field of animal traditional Chinese medicines and provides a compound traditional Chinese medicine transdermal spray for preventing and treating animal sarcopticacariasis and a preparation method of the transdermal spray aiming at solving the problems existing in the conventional chemical drugs and traditional Chinese medicine preparations for treating the animal sarcoptic acariasis. The transdermal spray is prepared by the following steps: mixing radix sophorae flavescentis, radix stemonae, Sichuan pepper, ethanol extract of radix euphorbiae lantu, melia azedarach, fructus kochiae, gallnut and jasmine mixed aqueous extract according to a certain ratio, adding water-soluble vitamins, glycerin, Tween 80, borneol, azone and propylene glycol according to a certain ratio for mixing, thereby obtaining the product. The transdermal spray is convenient to use, is capable of effectively transmitting through an epidermal layer of animal skin to kill sarcoptic mites in skin tunnels, and achieves the effects of clearing away heat and drying dampness, killing bacteria and diminishing inflammation, rapidly relieving itching, astringing to resist seepage, diminishing inflammation and relieving pain, controlling infection and promoting growth of new skin. The transdermal spray has the characteristics of broad spectrum, high efficiency, low toxicity, no residue, environmental friendliness, economy and convenience, is capable of effectively preventing and treating animal sarcoptic acariasis and can provide an effective drug and technical means for controlling the increasingly serious animal surface parasitic diseases at present.