The invention relates to a preservation culture solution of bacterial strain for efficiently degrading lignocellulose and a preparation method of the culture solution, belonging to the field of biotechnology. The culturing solution provided by the invention is characterized in that each 1L of deionized water contains the following materials: magnesium sulfate, ferric sulfate, ammonium nitrate, glutamine, sodium molybdate, cobalt chloride, potassium iodide, monopotassium phosphate, yeast powder, glucose, sulfuretted sodium glycollate, citric acid, starch, beef extract, asparagine and peach gum. The culturing solution is added with multiple nutrient elements which can promote the bacterial strain for degrading lignocellulose to rapidly and stably grow; the culturing solution is simple in preparation method, low in equipment investment, low in material cost and can accurately, stably and efficiently cultivate excellent bacterial strains with good adaptability; and the lignocellulose degrading activity of the obtained bacterial strain is higher than that of the bacterial strain naturally grown in natural world.