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Liquid-solid combined fermentation process for Bacteroides fragilis

A technology of Bacteroides fragilis and liquid-solid combination, which is applied in the field of liquid-solid combination fermentation technology, can solve the problems of few strains, complicated operation, waste of manpower and material resources, etc., and achieve the effect of high strain concentration, simple process and easy control

Inactive Publication Date: 2016-01-27
王军
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But now the strains are generally inoculated in liquid medium, and then fermented for 12-72 hours under anaerobic constant temperature state, and then filtered, washed, diluted, and tested for bacteria, so that the number of strains is less and the operation is complicated , wasting manpower and material resources

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] (1) First prepare the liquid culture medium, its weight percentage is peptone 0.5-2.5%, glucose 0.2-0.5%, cysteine ​​0.001-0.5%, NaCl 0.2-0.5%, NaHPO 4 0.1-0.3%, sodium thioglycolate 0.02-0.1%, beef liver extract 30-60%, beef extract 30-60%, yeast extract 0.1-0.8%;

[0018] (2) Primary strain culture

[0019] Use the above culture solution as the culture medium, put it into a narrow-mouth test bottle, sterilize at 121°C for 40 minutes, then inoculate 5% of the original seed culture, cover the culture medium with 0.5mm of sterile edible vegetable oil, and then cover it with a ground bottle cap Separate the seals, and finally place the culture vessel in a vacuum incubator at 38°C for 48 hours;

[0020] (3) Secondary strain culture

[0021] The culture medium and operation are the same as those for strain culture, but the petri dish is replaced with a colorless transparent reagent bottle. After inoculating 10% of the first-grade strain, shake for 1 minute every 5 hours, ...

Embodiment 2

[0025] 100 laying hens of the same species and age in the chicken farm were fed with the same feed under the same living conditions, randomly sampled, and divided into two groups of 50 each, and each chicken in group A took daily supplemented liquid The feed of the bacteria fermented by the solid process, each chicken in group B took the bacteria fermented by the liquid culture process every day, the number of bacteria added was the same, the test period was 2 months, and the feed with the additive was fed for one month, Feed the feed without additives for one month, and the egg production of group A increased by 17.53Kg relative to group B in two months.

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Abstract

The invention provides a liquid-solid combined fermentation process for Bacteroides fragilis. The process comprises a first step of preparing a liquid nutrient solution from, by weight, 0.5 to 2.5% of peptone, 0.2 to 0.5% of glucose, 0.001 to 0.5% of cysteine, 0.2 to 0.5% of NaCl, 0.1 to 0.3% of NaHPO4, 0.02 to 0.1% of sodium thioglycolate, 30 to 60% of liquid ox liver extract, 30 to 60% of liquid beef extract and 0.1 to 0.8% of yeast extract. The process provided by the invention employs liquid-solid combined fermentation, and a great amount of Bacteroides fragilis can be obtained in a short period of time by using the process; and the process has the advantages of easiness, small investment, easy controllability in operation, small possibility of infection with competitors due to implementation of all the operations under vacuum and aseptic conditions and high concentration of bacterial strains.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a liquid-solid combined fermentation process with Bacteroides fragilis as the main microecology. Background technique [0002] Bacteroides fragilis (b.fragilis) can decompose sugar and is resistant to bile. It is a representative strain of Bacteroides. Gram-negative bacilli with blunt rounded ends and dense staining, with a non-colored part in the middle. Obligate anaerobic, no spores, no motility, 0.5×1.3~1.6um in diameter. Bacteroides fragilis is an obligate anaerobic bacterium, hemin and 20% bile can promote its growth, weak biochemical reaction, and can decompose sugar. The hydrolytic enzyme reaction is positive, and the terminal metabolites of glucose include acetic acid and succinic acid; indole variable, bile salt resistance, and hydrolysis of escin. Does not reduce nitrates. Produce malate dehydrogenase, glutamate dehydrogenase, glucose 6 phosphate dehydrogenase (g6pdh),...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/01
Inventor 王军程亚龙翟建武
Owner 王军
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