The invention discloses an extracting process of biocontrol strains being fruit
pythium aphanidermatum and fruit
pythium aphanidermatum
toxin for prevention and treatment of skyblue broomrape
herb. The extracting process comprises enabling the fruit
pythium aphanidermatum preserved on an inclined plane to be activated on a PDA panel, picking bacterium blocks, collecting
hypha after the bacterium blocks are shaken and cultured in a PD culture medium, performing rotating
steaming concentration after
ethyl acetate solution is extracted in an
ultrasound circulating extraction meter, utilizing NaHNO3 solution to extract crude extract, transferring the crude extract into ethyl
ether solution, collecting aqueous phase, adjusting pH to utilize
petroleum ether solution to extract extract, collecting the aqueous phase again and performing extraction through
ethyl acetate, and performing constant volume of
methanol after the
ethyl acetate is decompressed and concentrated. The invention further discloses purposes of biocontrol strains and the
toxin of the biocontrol strains, and the purposes are for controlling and preventing root
parasitism weed skyblue broomrape
herb. The extracting process is suitable for unique geological weather conditions of Sinkiang, is used for effectively preventing root
parasitism weed skyblue broomrape
herb of cash crops, and is suitable for industrial massive production, economical, practical and low in cost.